博碩士論文 86622005 詳細資訊


姓名 宋平業(Pin-Yeh Sung)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 三氯乙烯及四氯乙烯對中國倉鼠卵巢細胞的毒性研究
(Toxicity study of Trichloroethylene and Tetrachloroethylene in CHO-K1 cells)
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摘要(中) 就中國倉鼠卵巢細胞對於三氯乙烯與四氯乙烯的毒性確認,是基於將細胞接種於活體外的揮發暴露系統中。在特製60 mm 培養皿中間區域部分,另外貼黏著一個25-mm 大小的玻璃培養皿,以提供加入揮發性藥劑所需要的區域。個別處理三氯乙烯或四氯乙烯的劑量分別是三氯乙烯20-80 ul ,四氯乙烯5-20 ul ;其中利用藥劑揮發後I變成氣體,再溶到培養液中。進而研究其影響細胞的生理與生化之反應。得到中國倉鼠卵巢細胞對三氯及四氯乙烯的毒性影響有:細胞的生長抑制、細胞內的酵素活性、細胞內GSH 的濃度等。其中明顯隨著添藥量增加而明顯增加的是:生長抑制現象與微小核增加頻率等。此外也發現在低劑量處理時 (三氯乙烯5-20 ul ,四氯乙烯1-5 ul) GSH 含量反而會隨之增加。增加量約為1.5 倍正常量。但當添藥量超過之後GSH 則是快速的下降。除此之外在GST 的活性方面,活性強度隨著處理藥劑濃度的增加,而有明顯的增加,上升的倍數約為三倍值。比較GSH 含量不同之後發現,隨著藥劑濃度增加,而並沒有顯著地改變細胞的生長抑制現象。在三氯與四氯乙烯對中國倉鼠卵巢細胞毒性上,GSH-conjugate 可能是一個中間反應;然而GSH-dependent 的活化作用在中國倉鼠卵巢細胞中,對三氯與四氯乙烯的biotransformation 上扮演了一個次要的解毒角色。
摘要(英) estimating chlorine contained organic solvents in contaminated soil and water can facilitate the overall understanding of anamal health risk. Currently, trichloroethylene (TCE) and tetrachloroethylene (PCE) are the major environmental contaminant and an occupational concern issues because of their widespread industrial use. The objective of this study was to use the cell culture techniques (Chinese hamster ovary cells, CHO-K1) to determine the toxicity of the toxicity of trichloroethylene (TCE) and tetrachloroethylene (PCE) on the CHO-K1. The CHO-K1 cells were grown in a 60-mm petri dish with a 25-mm glass dish glued in the central area. The TCE (20-80 ul) or PCE (5-20 ul) was added to the central glass well, and let it to evaporate and dissolve in the surrounding medium in
which cells were growing. Treatment of CHO-K1 cells with either TCE or PCE resulted in a dose and exposure inhibition of cell growth. A statistically significant increase in the frequency of MN (micronuclei test) was also observed in either TCE or PCE-treated CHO-K1 cells. Low dose of TCE (5-20 ul) or (1-5 ul) treatments showed significantly enhancement the intracellular glutathione (GSH) levels. However, the level of GSH rapidly decreased in the presence of higher dose of TCE (40-80 ul) or PCE (10-20 ul). Furthermore, the GST activity was enhanced by TCE- or PCE- treatment. Depletion of cellular GSH showed
no effect on the sensitivity of CHO-K1 cells to TCE- or PCE- treatments. The GSH-conjugate has been proposed as a reactive intermediate, which is account for the toxicity of TCE and PCE, however the GSH-dependent activation play a minor role in the biotransformation of TCE and PCE in CHO-K1cells.
關鍵字(中) ★ 三氯乙烯
★ 四氯乙烯
★ 細胞培養
★ GSH
★ Catalase
★ GST
★ 微小核
★ 揮發性有機溶劑
關鍵字(英) ★ trichloroethylene
★ tetrachloroethylene
★ cell culture
★ GSH
★ Catalase
★ GST
★ MN
★ VOC
論文目次 目錄
壹、 中文摘要… … … … … .… … … .. … … … … . … ..IV
貳、 英文摘要… … … … ….. .. … … .. … … … … . … ..VI
參、 緒論… … … … … … …… … ... … … . … … … … . 1
肆、 材料與方法.. … … … …. … .. … . … … . … … … .11
伍、 結果… … … … … … … … … … … … … … … … ...19
陸、 討論… … … … … … … .. … … … … … … … … ...23
柒、 圖表… … … … … … … … … … … … … … … … ...28
圖一、有機溶劑對細胞生長抑制的情形… …. … … … … … … .28
圖二、有機溶劑處理後24 小時生長觀察… …… … … … … … . 29
圖三、細胞毒性的分析… … … … … .. . … … .. … … … . 30
圖四、細胞內Glutathione 的分析… … … … … … … … … … 31
圖五、經BSO 前處理後再暴露在有機溶劑的結果(一) … …..... ..32
圖六、經BSO 前處理後再暴露在有機溶劑的結果(二) … …..... ..33
圖七、細胞Glutathione S Transferase (GST) activity 分析…...34
圖八、細胞內Catalase 的分析… … … … … … … … … … .35
圖九、微核的觀察… … … . … … … … . … … … … … … 36
圖十、酸鹼的變化現象… … . … … … … …. … … … … … .37
捌、 參考文獻… … … … … … .. … … … … … … … . … 38
玖、 附錄… … … … … … … .. … . … …… … … .. … ..46
參考文獻 參考文獻 (References)
註1 :中央日報,1998 年6 月01 日
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註2 :中央日報,1998 年6 月02 日
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註3 :中央日報,1998 年8 月13 日
http://www.cdn.com.tw/daily/1998/08/13/text/870813da.htm
註4 :中央日報,1998 年6 月22 日
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註5 :整治進度,網頁
http://www.epa.gov.tw/news/en880208.htm
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指導教授 李賢淇 審核日期 2000-7-19
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