三氯乙烯與四氯乙烯對人類肺癌細胞之毒性研究

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蔡欣怡(Shin-Yi Tsai) 查詢紙本館藏

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論文名稱

三氯乙烯與四氯乙烯對人類肺癌細胞之毒性研究
(Toxicity of Trichloroethylene and Tetrachloroethylene to Human Lung Cancer Cells)

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關鍵字(中)

★ H1299
★ 毒性研究
★ 人類肺癌細胞
★ 四氯乙烯
★ 三氯乙烯
★ H460
★ H322
★ GSH

關鍵字(英)

★ toxicity
★ GSH
★ H322
★ H460
★ H1299
★ tetrachloroethylene
★ human lung cancer cells

論文目次

中文摘要………………………………………………….I
英文摘要………………………………………………….II
目錄 …………………………………………………….III
圖表目錄 ………………………………………………VI
縮寫表…………………………………………………….VIII
第一章緒論
1.1 碳氯化物的簡介…………………………………….1
1.1.1 碳氯化物的來源與用途
1.1.2 三氯乙烯與四氯乙烯對環境之污染途徑
1.1.3 三氯乙烯之物化特性及其對人體的危害
1.1.4 四氯乙烯之物化特性及其對人體的危害.
1.2 三氯乙烯對動物之毒性……………………………..2
1.3 四氯乙烯對動物之毒性……………………………..3
1.4 三氯乙烯與四氯乙烯的代謝途徑…………………..4
1.4.1 三氯乙烯的代謝途徑
1.4.2 四氯乙烯的代謝途徑
1.5 三氯乙烯在體外培養的研究………………………..5
1.6 研究動機與目的……………………………………..5
1.6.1 研究動機
1.6.2 研究目的
第二章材料與方法
2.1 細胞株………………………………………………..7
2.2 細胞培養……………………………………………..7
2.3 藥劑處理法…………………………………………..8
2.3.1 氣相處理法
2.3.2 培養液中藥劑濃度檢測
2.4 細胞毒性檢測………………………………………...9
2.5 細胞群落分析三氯乙烯及四氯乙烯之毒性………..9
2.6 MTT細胞活性分析法……………………………..10
2.7 微核之分析…………………………………………..10
2.8 GSH之檢測………………………………………..11
2.8.1 細胞樣品前處理
2.8.2 GSH濃度測量之原理與方法
2.9 GST之活性測定……………………………………..11
2.9.1 細胞樣品前處理
2.9.2 GST活性測定之原理與方法
2.10 蛋白質定量………………………………………...12
第三章結果
3.1 培養液中揮發性有機溶劑濃度檢測………………...13
3.2 三氯乙烯及四氯乙烯對人類肺癌細胞之生長抑制作用.…………………………………………………..13
3.3 GSH對細胞之影響…………………………………...14
3.3.1 細胞密度與細胞內GSH含量之關係
3.3.2 三氯乙烯及四氯乙烯對細胞內GSH含量之影響
3.3.3 BSO抑制細胞內GSH之生成
3.3.4 細胞內GSH對三氯乙烯及四氯乙烯的毒性影響
3.4 三氯乙烯與四氯乙烯對H460細胞之GST酵素活性影響…………………………………………………...16
3.5 三氯乙烯與四氯乙烯對人類肺癌細胞之遺傳毒性...16
第四章討論 ……………………………………………..17
第五章結論與建議………………………………………20
參考文獻…………………………………………………..38
附錄………………………………………………………..45

參考文獻

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指導教授

黃榮南(Rong-Nan Huang)

審核日期

2000-7-20

推文