博碩士論文 87622013 詳細資訊




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姓名 鄭閔魁(Ming-Kui Cheng)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 生物除磷反應槽中可分離微生物菌相多樣性的探討
(The study of the isolated microbial divisity in the EBPR reactor)
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關鍵字(中) ★ 親源樹
★ 微生物分離法
★ 多樣性
★ 分類學
★ 微生物
★ 活性污泥
★ 生物除磷
★ 去氧核醣核酸
關鍵字(英) ★ 16S rDNA
★ Proteobacteria
★ Acinetobacter spp
★ DNA extract
★ DGGE
★ EBPR reactor
★ clone library
★ PCR
論文目次 目 錄
摘要………..……………………………………………………………....I.
英文摘要………………………………………………………………….II.
目錄………………………………………………………………………III.
圖目錄…………………………………………………………………….V.
表目錄…………………………………………………………………….VI.
第一章前言…………………………………………………………….1.
第二章文獻回顧……………………………………………………….3.
2.1 微生物菌相分析………………………………… …………….…3.
2.1.1核醣體去氧核醣核酸(ribosomal DNA, rDNA)
2.1.2 5S rDNA、16S rDNA與23S rDNA在演化上的角色
2.1.3 以16S rDNA為主的微生物菌種分類學
2.2 生物蓄磷除磷反應基本理論……………………………………..6.
2.3生物除磷活性污泥反應菌相探討………………………………...7.
2.3.1利用傳統分離法對活性汙泥中菌相探討
2.3.2 活性汙泥中的Acinetobacter spp.對生物除磷的影響
2.3.3 以16S rDNA為主的分子生物技術菌相分析.
2.3.3.1利用16S rDNA clone library
2.3.3.2利用螢光標記雜交探討
第三章實驗材料與方法………………………………………………15.
3.1 生物除磷活性污泥系統
3.2 細菌之分離與純化
3.3 細菌之增量與保存
3.4 DNA萃取
3.5 16S rDNA PCR
3.6 DGGE
3.7定序
3.8 親緣樹
第四章結果與討論…………………………………………………….19.
4.1 實驗結果
4.1.1菌株的分離
4.1.2菌株的篩選
4.1.3菌株分類學上的鑑定
4.2 討論
4.3. 結論
參考文獻…………………………………………………………………29.
附錄………………………………………………………………………52.
圖 目 錄
圖1.1 生物除磷系統整體實驗大綱….………………………………36.
圖3.1 本實驗之生物除磷反應槽設計圖..…………………….……38.
圖3.2 生物除磷反應槽代謝表現.……………………………………39.
圖3.3 實驗步驟簡圖…………………………………………….……40.
圖3.4 DGGE反應進行流程圖………………………………………..42.
圖4.1 生物除磷反應槽各菌組織DGGE凝膠電泳圖………….……45.
圖4.2 生物除磷反應槽之微生物菌相親源樹………………….……46
圖4.3 生物除磷反應槽可分離微生物96株菌之菌相比例圖...……47.
圖4.4 生物除磷反應槽以clone選殖之結果(Nielsen et al.,2000)…..48.
圖4.5 微生物分離法與非微生物分離法subdivision的比較………..49.
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指導教授 劉文佐 審核日期 2000-7-20
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