博碩士論文 88321032 詳細資訊




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姓名 何怡瑱( Yi- Chan Ho)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 三團聯聚合物(PF-127)和磷脂質間交互作用力量測和微脂粒穩定度之探討
(The study of interaction of PEO-PPO-PEO copolymer (PF-127) with phospholipid and comparison of physical stability of liposomes)
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摘要(中) 本研究主要探討25 下,DMPC/PF-127(重量比為34:4、34:14和34:34)之Gibbs’吸附單分子層和PF-127分子吸附於三種不同單分子層(DMPC、DMPC/Vit-E及DMPC/cholesterol)的氣/液界面行為。首先就DMPC/PF-127 Gibbs’吸附單分子層系統而言,主要進行平衡表面張力量測和表面壓-每分子佔據面積等溫線(Π-A isotherm)的實驗。在所得的結果中,平衡表面張力隨著 PF-127添加量的增加呈現正比的線性關係。配合Gibbs表面過剩濃度關係式,可得表面過剩濃度和分子佔據面積。混合單分子層表面壓-每分子佔據面積的量測中,隨著PF-127添加量的增加,等溫線向右平移且有相變區(liquid-expanded transition liquid-condensed)的發生。
PF-127分子吸附在DMPC、DMPC/Vit-E、DMPC/ cholesterol三種不同單分子層的行為,則是藉由量測表面壓隨時間的變化的量測和表面壓-每分子佔據面積等溫線來進行研究。PF-127吸附在DMPC單分子層系統中,不同起始表面壓之表面壓隨時間變化的結果顯示,平衡表面壓與PF-127添加量呈正比關係。對於DMPC/Vit-E和DMPC/cholesterol混合單分子層系統,平衡表面壓的範圍分別是從 15.8?28.3mN/m和 20.3~27.7mN/m,顯示添加維他命E和膽固醇改變分子間排列距離,使PF-127吸附於單分子層的表面壓產生差異。PF-127吸附平衡後之Π-A等溫線的結果顯示:(1)等溫線中有相變區的發生;(2)曲線向右平移,表示PF-127分子可能吸附或嵌入單分子層中,但因吸附的不夠緊或嵌入的不深,在高表面壓時,PF-127易被擠出單分子層中。此與微脂粒DSC實驗中,無論是嵌入法或吸附法都不影響相轉移溫度,推測PF-127嵌入脂質雙層不深是相符的。
摘要(英) This study investigated the behavior of mixed DMPC/PF-127 Gibbs’ adsorption monolayers (the weight ratio are 34: 4 , 34:14 and 34:34)and the PF-127 absorbed on different monolayers(the composition are DMPC, DMPC/Vit-E and DMPC/cholesterol)at the air/liquid interface at 25 . For mixed DMPC/PF-127 adsorption monolayers system at the air/water interface, the measurements of equilibrium surface tensions and surface pressure-area per molecule(Π-A) isotherms were carried out. The results of the mixed monolayer, the equilibrium surface tensions increased with the PF-127 concentration. But the excess of surface concentration is non- linear. TheΠ-A isotherms of the behavior of mixed DMPC/PF-127 monolayers indicate that the addition of the PF-127increased the shift percentages and the transition phases were obviously.
The behavior of the PF-127 adsorbed on different monolayers were investigated from the measurements of the change of surface pressure with time andΠ-A isotherms. In the PF-127 adsorption of monolayer systems, the equilibrium adsorption surface pressure increased with the PF-127 concentration and the initial surface pressure. The behavior of PF-127 adsorbed on DMPC/Vit-E and DMPC/cholesterol mixed monolayers, the ranges of equilibrium adsorption surface pressures are 15.8~28.3mN/m and 20.3~27.7mN/m, indicated that the addition of α-tocopherol and cholesterol change the distance of mixed monolayers. TheΠ-A isotherms of the PF-127 adsorbed to balance indicate that the transition phases were carried out and the PF-127 might be insert or absorb to monolayers. However, at higher surface pressure, the PF-127 molecules are squeezed into the subphase. From the DSC (differential Scanning Calorimetry ) experiments of liposome, the change of temperatures of phase transition regardless of the PF-127 incorporated or adsorbed to liposomes, which suggest the PF-127 does not insert or absorb deeply. Finally, the results obtained in this study support that the PF-127 can be readily absorbed or inserted onto liposome, providing steric barrier , preventing the fusion or aggregation of liposomes.
關鍵字(中) ★ 界面
★ 磷脂質
★ 單分子層
★ 微脂粒
關鍵字(英) ★ interface
★ phospholipid
★ monolayer
★ liposome
論文目次 目錄
中文摘要I
英文摘要II
目錄III
表目錄VI
圖目錄VII
符號說明XI
第一章 序論1
1-1 導論1
1-2 研究目的與動機3
第二章 文獻回顧4
2-1 三團連聚合物(PF-127)4
2-2 磷脂質(phospholipid)6
2-3 單分子層(monolayer)7
2-3-1 氣/液界面單分子層7
2-3-2 單分子層行為8
2-4 微脂粒(liposome)9
第三章 理論分析21
3-1 表面張力21
3-1-1 表面張力(surface tension)之定義 21
3-1-2 臨界微胞濃度(CMC)21
3-1-3 界面活性(surface activity)22
3-1-4 表面及界面之量測23
3-2 表面吸附行為理論- Gibbs’ adsorption equation(1928)24
第四章 實驗藥品與方法32
4-1 藥品32
4-2 方法33
4-2-1 表面張力的量測33
4-2-2 表面壓-時間量測33
4-2-3 表面壓-每分子佔據面積等溫的測量34
第五章 結果與討論41
5-1 DMPC和PF-127之Gibbs’吸附單分子層實驗41
5-1-1 DMPC、PF-127不同混合比例之表面張力實驗41
5-1-2 過剩濃度與每分子佔據面積42
5-1-3 DMCP和PF-127不同比例混合單分子層表面壓-每分子佔據面積等溫線43
5-2 PF-127分子吸附於不同單分子層的行為 44
5-2-1 單一組成之單分子層(純DMPC)44
5-2-1-1 PF-127吸附於DMPC單分子層之表面壓隨時間變化實驗44
5-2-1-2 表面壓-濃度之實驗46
5-2-1-3 PF-127吸附於DMPC單分子層平衡後之表面壓-每分子佔據面積等溫線圖46
5-2-2 PF-127吸附於混合單分子層(DMPC:Vit-E = 4:1,DMPC:cholesterol= 4:1(molar ratio))之行為49
5-2-2-1 PF-127吸附於DMPC/Vit-E混合單分子層之表面壓隨時間變化實驗49
5-2-2-2 PF-127吸附於DMPC/Vit-E混合單分子層平衡後之表面壓-每分子佔據面積等溫線50
5-2-2-3 PF-127吸附於DMPC/cholesterol混合單分子層平衡後之表面壓-每分子佔據面積等溫線51
5-2-2-4 PF-127吸附於DMPC/cholesterol混合單分子層平衡後之表面壓-每分子佔據面積等溫線52
5-2-3 起始表面壓為5mN/m,比較不同單分子層行為52
第六章 結論 77
參考文獻79
附錄85
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指導教授 陳文逸(Wen-yih Chen) 審核日期 2001-6-21
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