綠茶唲茶素對於前脂肪細胞分化的影響

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張欣蕙(Shin-Huei Chang) 查詢紙本館藏

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生命科學系

論文名稱

綠茶唲茶素對於前脂肪細胞分化的影響

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摘要(中)

脂質的積聚反映出前脂肪細胞分化為脂肪細胞，這過程受許多基因、賀
爾蒙(例如胰島素)以及營養因子(如維他命)所調節。本篇論文針對綠茶唲茶
素(曾被視為維他命P)對分化中脂肪細胞的影響及作用機制加以探討。我們
發現綠茶中的EGCG 在前脂肪細胞分化前期對於降低胰島素- MIX
(1-methyl-3-isobutylxanthine)-DEX (dexamethasone)誘發的前脂肪細胞
數目比其他結構相近的綠茶唲茶素如EGC、ECG、EC 效果明顯，且其抑制作
用具有劑量效應和時間效應。此外，EGCG 改變C/EBPβ、C/EBPα、PPARγ等能
有效刺激前脂肪細胞脂肪合成(adipogenesis)的分化相關轉錄因子之蛋白
表現，也減少細胞內和培養液中的三酸甘油酯總量。進一步研究發現，單獨
給予MIX 刺激造成PPARγ蛋白量顯著的增加，但此結果在綠茶唲茶素中受
EGCG 專一地以劑量相關作用抑制。H-89，一種蛋白激酶A(protein kinase A)
的抑制劑，並不能阻斷EGCG 抑制MIX 刺激的PPARγ表現，此結果支持EGCG
的作用並非經由蛋白激酶A 相關途徑(protein kinase A-associated
pathway)。由以上的結果，我們得到的結論為，EGCG 可能經由控制脂肪生成
相關的轉錄因子來調節前脂肪細胞的分化。

摘要(英)

Lipid accumulation reflects the process of preadipocytes differentiating into adipocytes
and such process is regulated by genetic, hormonal (i.e., insulin) and nutritional cues (i.e.,
vitamins). In this study, we examined the effect of green tea catechins, having been
considered as vitamin P, on differentiation of 3T3-L1 preadipocytes and investigated how they
work. We found that green tea EGCG was more effective than structurally-related EC, EGC
or ECG to reduce insulin-dexamethasone-1-methyl-3-isobutylxanthine (IDM)-induced
increases in cell number of preadipocytes during their initial differentiation to adipocytes.
The EGCG effect varied with dose administration and with time course. However, EGCG
altered the protein amount of C/EBPβ, C/EBPα, or PPARγ transcriptional factors, which were
enabling to stimulate adipogenesis of preadipocytes, as well as reduced total triglyceride
accumulation in the cells and medium. Further study showed that treatment of preadipocytes
with MIX alone, but neither insulin nor dexamethasone, caused a significant increase in the
protein amount of PPARγ and such stimulation was decreased by EGCG in the manner of dose
dependency or catechin specificity. H-89, a selective protein kinase A inhibitor, could not
block the EGCG inhibition of MIX-stimulated PPARγ, suggesting that EGCG effect is
independent of protein kinase A-associated pathway. We conclude that green tea EGCG may
modulate differentiation of preadipocytes to adipocytes through altering
adipogenesis-controlling transcriptional factors.

關鍵字(中)

★ 唲茶素
★ 脂肪細胞
★ 分化

關鍵字(英)

★ catechin
★ adipocyte
★ differentiation

論文目次

目錄
第一章：緒論5
一、3T3-L1 前脂肪細胞的分化5
二、脂肪細胞分化的指標5
三、調節脂肪細胞分化的重要因子6
四、綠茶調節脂肪細胞的生長與分化8
五、綠茶調節其他細胞株的分化及可的作用機制9
六、動機與目的10
第二章：材料與方法11
一、實驗材料11
二、實驗方法12
1. 細胞來源與培養12
2. 細胞處理12
3. 細胞計數方法13
4. 蛋白質濃度定量14
5. 西方點墨法14
6. 三酸甘油酯的測定15
7. 磷光螢光分析16
第三章：結果17
一、EGCG 減少分化中前脂肪細胞的數目17
二、EGCG 調節前脂肪細胞的分化18
三、EGCG 改變MIX 對於前脂肪細胞分化因子的調節20
四、EGCG 影響INS 對於前脂肪細胞分化因子的調節23
五、EGCG 影響DEX 對於前脂肪細胞分化因子的調節23
第四章：討論24
第五章：結論與未來展望27
第六章：參考文獻28
第七章：附錄31

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指導教授

高永旭(Yung-Hsi Kao)

審核日期

2003-7-24

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