砷化物誘導Thioredoxin Peroxidase II蛋白之表現及機制之研究

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林佩蓉(Pei-Jung Lin) 查詢紙本館藏

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論文名稱

砷化物誘導Thioredoxin Peroxidase II蛋白之表現及機制之研究
(The mechanism of Arsenic induce Thioredoxin Peroxidase II expression)

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摘要(中)

中文摘要
砷化物是廣泛存在於自然環境中的毒性物質，與人類皮膚癌、肺癌及肝癌的發生息息相關。近年來許多學者的研究指出，砷化物所造成的毒性與砷化物會產生自由基有關。在細胞中為了抵抗氧化壓力，細胞自己便會發展許多防禦的機制。Thioredoxin peroxidase II（TPxII）是一種受氧化性壓力（oxidative stress）誘導的酵素，也是一新發現的抗氧化蛋白質，其功能涵蓋細胞的分裂及分化、保護其他蛋白質免於受到氧化傷害、參與細胞中訊息的傳遞。
在本論文中，我們研究不同砷化物對各種哺乳類細胞中TPx II表現的影響及調控機制。由結果發現，在SA7N細胞中，亞砷酸鈉會誘發TPx II大量表現。而對其他細胞株例如：H1299、H460、HFW、IRC、KB、HaCaT等，亞砷酸鈉則無法誘導其TPx II大量表現。此外，也發現在亞砷酸鈉處理下，SA7N細胞內過氧化氫（H2O2）的累積量有增加的現象，顯示亞砷酸鈉誘導TPx II的表現，可能與其透過對細胞產生氧化性壓迫有關。
我們進一步以SA7N細胞研究亞砷酸鈉誘導TPx II表現的訊號傳遞途徑。結果顯示蛋白質激酶C（PKC）的抑制劑包括Rottlerin，Staurosprine可以抑制亞砷酸鈉所誘導TPx II的表現，而且在鈣離子
螯合劑EGTA和BAPTA/AM的處理下，亞砷酸鈉所誘導的TPx II大量表現也都沒有被抑制。進一步研究發現，對PKCδ有高專一性的抑制劑G06983、Calphostin C也可以有效地抑制亞砷酸鈉誘導TPx II的表現。而蛋白質激酶A（PKA）抑制劑（H89）、ERK抑制劑（PD98059）、p38抑制劑（SB203580）、酪胺酸激酶抑制劑（Genistein）則都無法抑制TPx II的表現。此一結果顯示，蛋白質激酶Cδ可能參與在SA7N細胞中亞砷酸鈉誘導TPx II的表現。

摘要(英)

Abstract
Arsenic is a common environmental toxicant associated with human skin, lung and liver cancers. Recently, numerous studies suggested that arsenic-induced toxicity is associated with the generation of free radicals. To cope with the oxidative stress, the cell has developed various defense mechanism. Thioredoxin peroxidase II (TPx II) is an oxidative stress-inducible enzyme. TPx II functions in cell proliferation and differentiation, and protects other proteins from oxidative damage.
In this study, we investigate the effect of arsenic compounds on the expression of TPx II. The results showed that arsenite induces TPx II protein expression in SA7N cell (a revertant of arsenic resistant Chinese hamster ovary cells), but not in other cell lines such as H1299、H460、HFW、IRC、KB、HaCaT. Moreover, the induction of TPx II also accompanied with H2O2 accumulation in SA7N cells. We therefore further explore the signaling pathway involved in TPx II induction by arsenite in SA7N cells. The results showed that pre-treatment of SA7N cells with inhibitors of protein kinase A (PKA), extracellular-regulated kinase (ERK), p38 and tyrosine kinase had no effect on the induction of TPx-II expression by arsenite. Calcium chelators, such as EGTA and BAPTA/AM, also showed no effect on TPx-II induction by arsenite. However, arsenite-induced TPx-II expression was dose-dependently reduced by two broad–based protein kinase C (PKC) inhibitors, Rottlerin and Staurosprine. Furthermore, two highly specific inhibitors of PKCδ, Go6983 and Calphostin C ,
also decreased arsenite-induced TPx II expression. The present results suggested that PKCδ might be involved in arsenite-induced TPx II expression in SA7N cells.

關鍵字(中)

★ thioredoxin peroxidase
★ 砷化物

關鍵字(英)

★ thioredoxin perxidase
★ Arsenic

論文目次

目錄
圖目錄……………………………………………………………...III
縮寫與全名對照表…………………………………………………V
英文摘要…………………………………………………………...VI
中文摘要…………………………………………………………...VIII
第一章、緒論
I. 砷化物的簡介……………………..……….……...……………..1
II. Thioredoxin Peroxidase II的簡介………………………………3
III. 研究動機與目的…………..……………………………………5
第二章、材料與方法
實驗材料
1.細胞株………………………..…………………………………7
2.細胞的培養…………………………………………….……….7
3.抗體…………………………………..……………….…………7
實驗方法
1. 細胞中 TPx II 蛋白質表現量的測定
1. 1細胞的收集…………..…….……………..…………………8
2. 1.2蛋白質定量………………………..………..……….…….8
1.3 SDS-PAGE膠體電泳分析（Sodium dodecyl sulfate polyacrylamide gel eletrophoresis）………………………..9
1.4. 抗體純化…..………………………………….……….……..9
1.4.1 硫酸銨沈澱法………………………………………………9
1.5西方點墨法（Western blotting）……………………………10
3. 不同蛋白質抑制劑對亞砷酸鈉誘導TPx II蛋白質表現
的影響…………………………………………………………10
3. 細胞內過氧化氫累積量測定（Flow Cytometry Assay）…...11
第三章、結果………………………………………………………...12
第四章、討論………………………………………...….……………17
第五章、參考文獻…………………………………...…..………….38
圖表………………………………………………...…….…….………22
附錄………………………………………………..…………………...47
圖目錄
圖一、亞砷酸鈉對不同細胞株中TPx II蛋白質的表現之
影響………………………………………………………...22
圖二、三氧化二砷對不同細胞株中TPx II蛋白質表現之
影響…………………………………………………………23
圖三、五價砷酸鈉對不同細胞株中TPx II蛋白質表現之
影響…………………………………………………………24
圖四、亞砷酸鈉誘導SA7N細胞中TPx II蛋白質表現之
劑量效應……………………………………………………25
圖五、亞砷酸鈉誘導SA7N細胞中TPx II蛋白質表現之
時間效應……………………………………………………26
圖六、蛋白質激酶A（PKA）抑制劑對亞砷酸鈉誘導TPxII蛋白質的表現的影響………………………………………………27
圖七、 ERK和p38抑制劑對亞砷酸鈉誘導TPx II蛋白質的表現之影響…………………………………………………………28
圖八、酪胺酸激酶（tyrosine kinase）抑制劑對亞砷酸鈉誘導TPxII蛋白質的表現的影響………………………………………29
圖九、蛋白質激酶C（PKC）抑制劑對亞砷酸鈉誘導TPx II蛋白質的表現的影響………………………………………………30
圖十、蛋白質激酶C（PKC）抑制劑對亞砷酸鈉誘導TPx II蛋白質的表現之影響….……………………………………………....31
圖十一、蛋白質激酶C（PKC）抑制劑對亞砷酸鈉誘導TPx II蛋白質的表現的影響……………………………………………32
圖十二、蛋白質激酶C（PKC）抑制劑對亞砷酸鈉誘導TPx II蛋白質的表現的影響……………………………………………33
圖十三、鈣離子螯合劑對亞砷酸鈉誘導TPx II蛋白質的之影響……………………………………………………………34
圖十四、 Catalase抑制劑3-Amino-1,2,4-triazole（3-AT）對亞砷酸鈉誘導TPx II蛋白質的表現之影響………………………35
圖十五、 Glutathione Peroxidase抑制劑mercaptosuccinic acid（MS）對亞砷酸鈉誘導TPx II蛋白質的表現之影響……………..36
圖十六、亞砷酸鈉誘導SA7N細胞累積過氧化氫之分析…………..37

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指導教授

黃榮南(Rong-Nan Huang)

審核日期

2003-7-14

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