博碩士論文 91222003 詳細資訊




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姓名 蔡孟哲(Meng-Tse Tsai)  查詢紙本館藏   畢業系所 物理學系
論文名稱 利用雷射破壞方法研究神經網路的連結及同步發火的行為
(A Study of Neuronal Network Connectivity by Photolysis)
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摘要(中) 神經網路中神經細胞之間的連結及訊息傳遞是個複雜的過程。鈣離子在神經網路中扮演著傳遞及調控神經細胞之間的訊息傳遞,利用鈣離子螢光造影的技術,我們可以觀察到神經網路中會有同步發火的現象。在這篇論文中,我們取下大鼠大腦皮質的神經細胞做體外培養,並利用紫外光雷射破壞神經網路中的細胞來研究神經細胞叢集大小與同步發火頻率之間的關係。另外鈣離子波的產生也出現在破壞神經細胞的過程,我們發現神經網路中鈣離子波的傳遞行為類似於擴散行為,但其擴散係數比自由的鈣離子大上一個數量級。我們亦分析其他實驗室對神經網路中神經膠質細胞Müller cell的鈣離子波的研究,一樣也可以得到類擴散行為的結果,只不過Müller cell的鈣離子波擴散係數較我們實驗中神經細胞的鈣離子波擴散係數要來的小。
摘要(英) The neural network connectivity is very complex. Calcium ion helps a neuron singal other neurons in neural network. In this thesis, cortical neurons from rat embryos are cultured to form neural networks. We observe the phenomenon of synchronous firing(SF) and use UV laser to destroy neuron network to see how does it changes in SF frequency. We also induce calcium waves in neuron network by UV laser killing. The behavior of calcium wave is like a diffusion process, but the diffusion constant measure in our experiment is much bigger than diffusion constant of free calcium ion.
關鍵字(中) ★ 神經網路
★ 訊息耦合長度
★ 鈣離子波
關鍵字(英) ★ calcium wave
★ neural network
★ synchronous firing
★ coupling distance
論文目次 摘要 I
目錄 II
圖目 III
第一章 簡介 1
1.1 神經網路背景介紹.................................1
1.2 神經網路同步發火特性.............................7
1.3 神經發火實驗的變數及調控方法.....................9
第二章 實驗裝置及實驗流程 13
2.1 顯微鏡..........................................14
2.2 螢光物質及ICCD..................................16
2.3 溫控設備........................................17
2.4 脈衝紫外雷射....................................19
2.5 光路配置........................................20
2.6 樣本製作流程....................................22
2.7 自動控制........................................24
第三章 神經網路發火之臨界尺度 26
3.1 動機及目的......................................26
3.2 神經網路同步發火之基本現象......................26
3.3 雷射破壞神經網路校正及流程......................28
3.4 實驗結果分析....................................30
第四章 神經網路之鈣離子波傳遞 35
4.1 鈣離子波傳導距離函數............................36
4.2 鈣離子波影響同步發火之現象......................44
4.3 實驗結果分析及待解問題..........................47
第五章 結論 48
參考資料 49
附錄A 藥品配方及解剖詳細流程圖 52
附錄B 分析程式 67
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指導教授 黎璧賢、陳志強
(Pik-Yin Lai、Chi-Keung Chan)
審核日期 2004-7-14
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