氧化性壓迫誘導Thioredoxin peroxidase II蛋白之表現機制研究

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李怡慧(Yi-Hwe Lee) 查詢紙本館藏

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論文名稱

氧化性壓迫誘導Thioredoxin peroxidase II蛋白之表現機制研究
(Mechanism of oxidative stress-induced Thioredoxin peroxidase II expression)

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摘要(中)

中文摘要
Thioredoxin peroxidase II (TPx II)是一種氧化壓力所誘導的酵素，具有保護其他蛋白質免於受到氧化傷害的功能，也參與細胞分裂及分化的訊息傳遞。砷化物暴露可能導致人類皮膚癌、肺癌、肝癌及心血管疾病的發生。近年來研究指出，砷化物的毒性與自由基有關；細胞為了抵抗氧化壓力，會啟動許多防護機制。
本研究探討亞砷酸鈉(As(III))及氧化性壓迫對老鼠纖維母細胞(3T3) TPx II表現的影響及調控機制。結果顯示As(III)處理可誘導老鼠纖維母細胞TPx II蛋白及mRNA之表現。此外其他氧化性藥劑(H2O2, CdCl2, DPPH)的處理，皆可造成TPx II蛋白表現量增加，而H2O2及DPPH處理，亦可造成TPx II mRNA之表現增加。
本研究進一步研究As(III)誘導TPx II表現之機制；結果顯示ERKs磷酸化受到As(III)處理明顯增加，蛋白質激酶C (PKC)的抑制劑(Staurosprine、Calphostin C) ，可以抑制As(III)所誘導TPx II的表現，鈣離子螯合劑EGTA對As(III)所誘導TPx II表現並沒有抑制作用，此外，Rotterlin (PKC δ專一性抑制劑)也能有效抑制As(III)所誘導TPx II表現。然而這些蛋白質激酶C (PKC)抑制劑對過氧化氫所誘導TPx II表現不具抑制作用，此一結果顯示As(III)誘導老鼠纖維母細胞TPx II表現的機制與PKC δ有關，並與H2O2和DPPH明顯不同。此外TPx II驅動子位於269-169鹼基位置，對As(III)誘導TPx II表現具有重要之影響；且藉由FKHR調控因子(與氧化性壓迫有關的調控因子) 可有效提高As(III)誘導TPx II轉錄活性。FKHR對H2O2, CdCl2, DPPH誘導TPx II轉錄並沒有影響。此一結果顯示As(III)誘導TPx II表現機制可能與H2O2, CdCl2, DPPH明顯不同，且與其對細胞產生氧化性壓迫有關。

摘要(英)

Abstract
Thioredoxin peroxidase II (TPx II) is an oxidative stress-inducible enzyme that functions in cell proliferation and differentiation, and protects other proteins from oxidative damage. Arsenic is an environmental pollutant associated with human skin, lung, liver cancers and cardiovascular diseases. Recent studies suggested that arsenic-induced toxicity is associated with the generation of free radicals. This study investigates the mechanism of sodium arsenite (As(III))- and other oxidative stress-induced TPx II expression in murine fibroblast cells (3T3). The results showed that As(III), H2O2, CdCl2 and DPPH dose-dependently induced TPx II protein expression in 3T3 cell. The mRNA level of TPx II were also induced by As(III), H2O2, and DPPH, but not CdCl2. In addition, the phospho-ERK protein level was significantly enhanced in response to As(III) treatment. The As(III)-induced TPx II expression could be dose-dependently abolished by the presence of Rotterlin (PKC δ specific inhibitor); however, Rotterlin showed no effect on H2O2- and DPPH-induced TPx II expression. We further investigated the effect of As(III) on TPx II expression in transcription level. The results showed that a DNA response element located at TPx II promoter 269-169 pair was critical for As(III) inductory effect on TPx II gene expression. The As(III)-induced TPx II expression was further enhanced by another transcriptional factor FKHR that had no effect on H2O2-, CdCl2- and DPPH-induced TPx II expression. These results indicated that the mechanism of As(III) induces TPx II protein expression likely through oxidative stress pathway by which is dependent on FKHR and distinct from that induced by H2O2, CdCl2 and DPPH.

關鍵字(中)

★ 亞砷酸鈉
★ 抗氧化性蛋白

關鍵字(英)

★ Thioredoxin peroxidase II
★ Arsenic

論文目次

Contents
I. Introduction 01
1. Arsenic 01
2. Reactive oxygen species 03
3. Thioredoxin peroxidase 04
4. Preliminary studies and specific aims 07
II. Materials and Methods 09
1. Cell culture and preparation of cell extract 09
2. Materials 09
2.1 Chemicals and Inhibitors 09
2.2 Plasmids 09
3. Western blot analysis 09
3.1 Protein quantitation 09
3.2 Gel electrophoresis 10
3.3 Western blot 10
4. Construction of pTPx II-Luc reporter plasmid 11
4.1 Genomic DNA extraction 11
4.2 Polymerase Chain Reaction 11
4.3 Subcloning 12
4.4 Linearization of expression vector 12
4.5 Ligation 13
4.6 Tramsformation 13
4.7 Screening 13
4.8 Extraction of plasmid DNA 14
5. RT-PCR analysis 14
5.1 RNA isolation 14
5.2 PCR 14
6. Assessment of Reporter Gene Activity 15
6.1 Transient transfection 15
6.2 Luciferase activity 15
6.3 b-galactosidase activity 15
III. Results 17
1. The effect of oxidant inducers on the expression level
of TPx II protein in 3T3 fibroblasts 17
2. The effect of oxidant inducers on the expression level
of TPx II mRNA in 3T3 fibroblasts 17
3. Mechanism of As(III)-induced TPx II expression 19
4. Construction of TPx-II promoter reporter gene 20
5. Effect of As(III) on the induction of TPx II promoter-
luciferase reporter constructs 21
6. Regulation of TPx II promoter activity by cotransfected
FKHR induced by As(III) 22
IV. Discussions 24
1. Signaling pathway of TPx II expression 24
2. Regulation of TPx II promoter activity 25
V. References 27
VI. Figures 38
1. Effect of As(III), H2O2, CdCl2 and DPPH on TPx II expression
in 3T3 cells 38
2. Effect of As(III), H2O2, CdCl2 and DPPH on TPx II mRNA
expression in 3T3 cells 39
3. Effect of As(III) on the activation of phosphorylated ERK1/2,
JNK, p38 MAPK and Akt in 3T3 cells 40
4. ERK inhibitor suppresses the induction of TPx II protein
by As(III) 41
5. PKC inhibitor suppresses the induction of TPx II protein
by As(III) 42
6. Effect of PKC δ inhibitor and EGTA on the induction of
TPx II protein by As(III) 43
7. Effect of protein kinase C inhibitor on the induction of
TPx II protein by H2O2 44
8. Effect of PKC δ inhibitor and EGTA on the induction of
TPx II protein by H2O2 45
9. Effect of PKC δ inhibitors on the induction of TPx II protein
by CdCl2. 46
10. Effect of PKC δ inhibitors on the induction of TPx II protein
by DPPH. 47
11. Construction of TPx II promoter-Luciferase reporter gene 48
12. Effect of As(III) on the induction of TPx II promoter-
luciferase reporter constructs 50
13. Effect of FKHR cotransfection on the induction of TPx II
promoter activity by As(III) 51
14. Effect of FKHR transfection on the induction of TPx II
mRNA by As(III) 52
15. Effect of FKHR cotransfection on the induction of TPx II promoter
activity by H2O2, DPPH and CdCl2 53

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指導教授

黃榮南(Rong-Nan Huang)

審核日期

2004-7-19

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