博碩士論文 91226024 詳細資訊




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姓名 葉星輝(Hsing-Hui Yeh)  查詢紙本館藏   畢業系所 光電科學與工程學系
論文名稱 生物晶片之螢光光學檢測
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摘要(中) 近幾年來,由於生物科技一日千里,並且搭配成熟的微系統加工技術,使得生物晶片的研究蔚為風潮。其中微流體晶片將實驗室中進行的一連串生化分析,整合至同一晶片中,相較於傳統實驗室,具有反應速度快,樣本及試劑使用量減少等不少優點,因此是未來生物晶片發展的重要方向之一。
而目前在微流體晶片中,所使用的螢光檢測技術,其螢光染料的激發頻譜與放射頻譜通常會有部份重疊在一起,因此在使用濾光片將激發光濾去以增加訊雜比的同時,也會把部份的放射光濾掉,將使得檢測更困難。本文中針對此問題,提出一種新型的檢測架構,把微流道與微光學元件(包含微透鏡及微光柵)作一系統性的整合。
初步以氦氖雷射以及綠光雷射進行測試,使用本生物晶片的架構,並且搭配CCD的檢測方式,可得到一個空間的頻譜訊號分佈。進一步再針對混合光譜的訊號進行分析,期望可以得到原先光譜的混合比例。
關鍵字(中) ★ 微光柵
★ 微透鏡
★ 微流道
★ 螢光
★ 生物晶片
關鍵字(英) ★ micrograting
★ biochip
★ fluorescence
★ microchannel
★ microlens
論文目次 目錄
論文摘要 i
目錄 ii
圖索引 iv
表索引 vi
第一章 緒論 1
1.1 前言 1
1.1.1 生物晶片之近況 2
1.1.2 微流體晶片之檢測方法 15
1.1.3 研究動機 16
1.2 論文架構 17
第二章 晶片架構設計 18
2.1繞射式透鏡的設計 20
2.2繞射式光柵的設計 26
2.3總結 31
第三章 元件製作 32
3.1 濕式蝕刻的製作方式 32
3.2 微流道的製作 34
3.3 繞射式透鏡的製作 40
3.4 繞射式光柵的製作 44
3.5 元件接合 47
3.6總結 50
第四章 量測結果與討論 51
4.1 蝕刻速率的量測 51
4.2 繞射式光柵的量測 53
4.3 頻譜訊號的量測 56
4.4混合訊號的量測 58
4.5總結 67
第五章 結論 68
參考文獻 70
參考文獻 參考文獻
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指導教授 楊宗勳(Tsung-Hsun Yang) 審核日期 2005-7-25
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