生物晶片螢光檢測之光源模型探討

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姓名

周虹宇(Hung-Yu Chou) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

生物晶片螢光檢測之光源模型探討
(Study of Light Source Model for Fluorescence Detection in Biochip)

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摘要(中)

近年來，由於生物技術突飛猛進的發展、再加上微機電系統技術與封裝技術不斷地持續的發展並漸趨成熟，因此跨領域整合式實驗室型生物晶片(Lab on a chip)的研究也隨之興起，並且已成為新世紀以來最為熱門的研究課題之一。
目前應用在生物晶片上的訊號偵測方法有許多種，例如：螢光檢測法、反射率量測法、電流檢測法和電位量測法等等，我們主要探討的是螢光檢測法，利用漸逝波(evanescent wave)的激發方式激發螢光，進而利用微光學元件調制螢光光源，而促成整個生物晶片檢測系統。
本文中，我們從系統基本的架構，包括經由不同的入射光所造成的漸逝波模擬和螢光初始光源模型的建立，一直到建立考慮散射效應的螢光光源模型以及初步的微小化收光元件之設計，而完整地建立出整個生物晶片檢測系統的雛型。未來，我們將可進一步更有效率地改進在微光學件上的設計，以完整建立整個生物晶片檢測系統。

摘要(英)

Recently, according to the great progress in biotechnology and the miniaturization techniques in engineering, the development on the technology of lab on a chip becomes one of the most important subjects for the multi-discipline integration.
There are many signal detection methods applied on the biochip, such as the fluorescence detection method、the reflectance detection method、the conductimetric detection method and the potentiometric detection method etc. Here, we focus on the fluorescence detection method and utilize the evanescent wave to excite the fluorescence, then use micro-optic elements to modulate the fluorescent light source and promote the detective system of the biochip.
In this thesis, it has been established the fundamental construction of the miniature detection system including evanescent wave arising from different types of incident light and the initial fluorescence emission model to the fluorescence emission considered scattering effect and the preliminary work of lens design. In the future, it will be further improved on the work of micro-optical elements, including micro-grating that combines with the miniature detection system. Finally, we hope to build up the complete miniature detection system.

關鍵字(中)

★ 螢光檢測
★ 生物晶片

關鍵字(英)

★ Lab-on-a-chip
★ fluorescence

論文目次

Chapter 1 Introduction to Lab-on-a-chip..............1
1-1 Introduction.....................................1
1-2 Laser Induced Fluorescence(LIF)..................6
1-3 Detection System Miniaturization.................8
Chapter 2 Properties of Fluorescence.................13
2-1 Introduction.....................................13
2-2 The Processes of Fluorescence Emission...........19
2-3 Absorption and Emission spectra..................19
2-4 Intensity of Fluorescence Emission...............21
2-5 Summary..........................................24
Chapter 3 Design of Miniature Detection System.......25
3-1 Total Internal Reflection(TIR) Configuration.....25
3-2 Evanescent Wave Excitation.......................27
3-3 Laser Induced Fluorescence Emission..............33
3-4 Light Scattering.................................35
3-5 Summary..........................................38
Chapter 4 Simulation Results.........................40
4-1 Evanescent Wave Excitation.......................40
4-2 Laser Induced Fluorescent Light..................42
4-3 Light Scattering.................................47
4-4 Micro-optic System...............................54
4-5 Summary..........................................70
Chapter 5 Future Work and Conclusion.................72
Reference............................................74

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指導教授

楊宗勳(Ｔsung-Hsun Yang)

審核日期

2004-7-16

推文