博碩士論文 92224004 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:11 、訪客IP:3.236.16.13
姓名 吳敏華(Min-Hua Wu)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 辛基苯酚對3T3-L1脂肪細胞中resistin的調節作用
(Octylphenol regulation of resistin in 3T3-L1 adipocyte)
相關論文
★ 中華鱉腦垂體甘丙氨激素之研究:cDNA選殖、表現及調控★ 綠茶表沒食子酸酯型唲茶素酸酯對胰島素刺激前脂肪細胞增生的抑制
★ FoxO1 調節抗胰島素激素基因的表現★ 綠茶表沒食子唲茶素沒食子酸酯受器對於人類乳癌細胞株MCF7生長的影響
★ 綠茶表沒食子酸酯型唲茶素酸酯抑制第一型内皮素作用於脂肪細胞上攝入葡萄糖的訊息機制★ 綠茶表兒茶素藉由microRNA-494路徑改善橫向主動脈繃紮術誘導型小鼠的心臟疾病
★ 內皮素誘導前脂肪細胞生長的訊息路徑★ 綠茶對前脂肪細胞生長的影響
★ 綠茶唲茶素對由第一型類胰島素所調節前脂肪細胞生長的影響★ 綠茶唲茶素對於前脂肪細胞分化的影響
★ Cdk2在綠茶唲茶素調節3T3-L1前脂肪細胞的生長和細胞凋亡扮演著必要性的角色★ 綠茶唲茶素透過MAPK相關途徑抑制3T3-L1前脂肪細胞的生長
★ 第一型類胰島素生長因子、綠茶唲茶素及雌性素對3T3-L1脂肪細胞中resistin的基因表達有不同的調節效果★ 綠茶唲茶素對前脂肪細胞內活性氧及榖胱甘肽的影響
★ 胰島素接受器受質在綠茶唲茶素對胰島素刺激前脂肪細胞生長作用中扮演的角色★ 賜諾殺穩定性及東方果實蠅費洛蒙之研究
檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   至系統瀏覽論文 ( 永不開放)
摘要(中) 抗胰島素激素(resistin)是由脂肪細胞所分泌的荷爾蒙,其作用會降低了胰島素刺激脂肪細胞攝入葡萄糖的能力,而造成胰島素抗性,並且連結肥胖症和第二型糖尿病之間的關係。抗胰島素激素的表達量會受到許多荷爾蒙的調節,但整個調控機制仍然不清楚。然而環境荷爾蒙是由許多環境化合物所代謝產生,並且在累積中會影響到生物體內原本的生理活性,其中辛基苯酚(OP)結構和雌性素相似,並且會模仿雌性素的作用。故在本論文中,利用3T3-L1脂肪細胞做為研究細胞株,本研究發現OP會增加抗胰島素激素mRNA及蛋白質的產生,並且隨著OP的濃度及處理時間的不同而有程度上的差異。而在前處理轉錄抑制劑actinomycin D及轉譯抑制劑cycloheximide的情況下,OP刺激抗胰島素激素mRNA及蛋白質的表達量並不會受到改變,因此OP會增加抗胰島素激素mRNA及蛋白質的穩定性。實驗中也證實OP增加抗胰島素激素mRNA及蛋白質的表達會受到雌性素接受器抑制劑ICI182780及MEK1抑制劑U0126所抑制,顯示OP會經由ERα及MEK1訊息傳導途徑增加抗胰島素激素的產生,然而OP也會影響到ERα和抗胰島素激素負調節因子PPARγ之間的結合能力和PPARγ蛋白質表現,是否OP增加抗胰島素激素的產生之機制也與PPARγ的途徑有直接的關聯,將有待以釐清。
摘要(英) Resistin (Rstn) is an adipocyte-specific secretory hormone that can reduce the insulin-stimulated glucose uptake by adipocytes, resulting in insulin resistance, and link the obesity and type II diabetes. It can be regulated by many hormones, but the exact mechanisms of their action is still not clear. Environment hormones are derived from the metabolites of many environmental chemical compounds and have the biological activity in organisms. Octylphenol (OP) is one of environmental estrogens that has the structural similarity to estrogen and can mimic the action of estrogens. In this study using 3T3-L1 adipocytes as the research cell line, OP induced increases in Rstn mRNA and protein expression with significantly dose- and time-dependent differences was discovered. Also, pretreatment with either transcriptional inhibitor actinomycin D or translational inhibitor cycloheximide did not prevent the OP-stimulated Rstn mRNA and protein expression. This suggests that OP increases the stabilities of Rstn mRNA and protein. In addition, pretreatment with either estrogen receptor alpha (ER?) inhibitor ICI182780 or MEK1 inhibitor U0126 prevented the OP-induced increases in Rstn mRNA and protein expression, suggesting that the effect of OP requires the functional ER? and MEK1. Moreover, OP increased PPAR? (a negative regulator of Rstn) protein expression and the association of ER? with PPAR?. Further studies to demonstrating whether the effect of OP on Rstn gene expression is mediated directly via modulation of PPAR? is needed.
關鍵字(中) ★ 辛基本酚
★ 抗胰島素激素
★ 脂肪細胞
關鍵字(英) ★ Octylphenol
★ resistin
★ adipocyte
論文目次 目錄 ------------------------------------------------ Ⅰ
圖目錄 ---------------------------------------------- Ⅳ
縮寫及全名對照表 ------------------------------------ Ⅴ
第一章:緒論 -------------------------------------------------------------- 1
一、 抗胰島激素 (resistin) ------------------------------------------- 1
二、 環境荷爾蒙(Environmental hormone) ------------------------------- 3
三、環境雌性素 ----------------------------------------------------------- 7
四、辛基苯酚 ------------------------------------------------------------- 8
五、雌性素影響resistin蛋白及基因的表達 ----------------------------------- 11
六、雌性素的分子作用機制與訊息傳導途徑 ----------------------------------- 12
七、研究動機問題與目的 --------------------------------------------------- 12
第二章 : 材料與方法 ------------------------------------------------------- 14
一、材料 ------------------------------------------------------------------ 14
二、實驗方法 -------------------------------------------------------------- 16
1. 細胞培養---------------------------------------------------------- 16
2. 細胞處理 --------------------------------------------------------- 16
3. 細胞計數方法 ----------------------------------------------------- 17
4. RNA 萃取---------------------------------------------------------- 17
5. 反轉錄酶反應(Reverse Transcriptase, RT) -------------------------- 18
6. 聚合酶鏈反應(Polymerase chain reaction, PCR) ----------------------18
7. PCR產物的分析 ---------------------------------------------------- 19
8. 蛋白質濃度定量 --------------------------------------------------- 20
9. 西方點墨法(Western blot analysis) -------------------------------- 20
10. 蛋白質免疫沉殿法 (Immunoprecipitation) --------------------------- 21
11. Enzyme-linked immunosorbent assay (ELISA) ------------------------ 22
12. 統計分析 --------------------------------------------------------- 23
第三章:結果 -------------------------------------------------------------- 24
一、 OP增加resistin mRNA表現及穩定度 ---------------------------------- 24
二、 OP增加resistin protein的表現 ------------------------------------- 24
三、 OP改變resistin表現的機制 ---------------------------------------- 25
1、 OP利用細胞原本的蛋白來產生resistin ------------------------------- 25
2、OP經由ERα的途徑改變resistin mRNA的表現 --------------------------------- 26
3、OP經由MAPK來促進resistin的表現 ----------------------------------------- 27
4、C/EBPα及PPARγ在OP促進resistin表現所扮演的角色--------------------------- 28
四、 環境雌性素與雌性素對resistin的作用 ------------------------------- 29
五、 OP對3T3-L1細胞數目的影響 ----------------------------------------- 29
第四章:討論 -------------------------------------------------------------- 31
第五章:結論與展望 ------------------------------------------------------- 35
第六章:參考文獻 ---------------------------------------------------------- 36
第七章:附錄 -------------------------------------------------------------- 54
附錄一:溶液配制 ---------------------------------------- 54
附錄二:其他數據 --------------------------------------- 57
附錄三:Resistin DIG PCR ELISA ------------------------------------- 60
參考文獻 1. Borgert CJ, LaKind JS, Witorsch RJ., A critical review of methods for comparing estrogenic activity of endogenous and exogenous chemicals in human milk and infant formula. Environ Health Perspect, 2003. 111: 1020-1036.
2. Brown R, Wiesner G, Ur E, Wilkinson M., Pituitary resistin gene expression is upregulated in vitro and in vivo by dexamethasone but is unaffected by rosiglitazone. Neuroendocrinology, 2005. 81: 41-48.
3. Calabro P, Samudio I, Willerson JT, Yeh ET., Resistin promotes smooth muscle cell proliferation through activation of extracellular signal-regulated kinase 1/2 and phosphatidylinositol 3-kinase pathways. Circulation, 2004. 110: 3335-3340.
4. Ciocca, D. R., Roig, L. M. V., Estrogen receptors in human nontarget tissues: biological and clinical implications. Endocr Rev., 1995. 16: 35-62.
5. Claire M. Steppan, Juan Wang, Eileen L. Whiteman, Morris J. Birnbaum, Mitchell A. Lazar, Activation of SOCS-3 by Resistin. Molecular and Cellular Biology, 2005. 25: 1569-1575.
6. CM., Klinge, Estrogen receptor interaction with estrogen response elements. Nucleic Acids Res, 2001. 29: 2905-2919.
7. Dal-Ho Han, Michael S. Denison, Hirofumi Tachibana, Koji Yamada, Relationship between Estrogen Receptor-Binding and Estrogenic Activities of Environmental Estrogens and Suppression by Flavonids. Biosci. Biotechnol. Biochem., 2002. 66: 1479-1487.
8. Dos Santos EG, Dieudonne MN, Pecquery R, Le Moal V, Giudicelli Y, Lacasa D., Rapid nongenomic E2 effects on p42/p44 MAPK, activator protein-1, and cAMP response element binding protein in rat white adipocytes. Endocrinology, 2002. 143: 930-940.
9. Eilertdon CD, Sheridan MA, Pancreatic somatostatin-14 and somatostatin-25 release in rainbow trout is stimulated by glucose and arginine. Am J Physiol, 1995. 269: R1017-1023.
10. Eing-Mei Tsai, Shao-Chun Wang, Jau-Nan Lee, Mien-Chie Hung, Akt activation by estrogen in estrogen receptor-nagative breast cancer cells. Cancer reserach, 2001. 61: 8390-8392.
11. Eulalia Pozo-Guisado, M. Jesu’s Lorenzo-Benayas, Pedro M. Ferandez-Salguero, Resveratrol modulates the PI-3K pathway through an estrogen receptor α-dependent mechanism: relevance in cell proliferation. Int J Cancer, 2003. 109: 167-173.
12. Fasshauer M, Paschke R, Regulation of adipocytokines and insulin resistance. Diabetologia, 2003. 46: 1594-1603.
13. Florence Machinal, Marie-Noelle Dieudonne, Marie-Christine Leneveu, Rene Pecquery and Yves Giudicelli, In Vivo and in Vitro ob Gene Expression and Leptin secretion in rat adipocytes: Evidence for a regional specific regulation by sex steroid hormones. Endocrinology, 1999. 140: 1567-1574.
14. Francoise Paris, Patrick Balaguer, Beatrice Terousnne, Nadege Servant, Caroline Lacoste, Jean-Pierre Cravedi, Jean-Claude Nicolas, Charles Sultan, Phenylphenols, biphenols, bisphenol-A and 4-tert-octylphenol exhibit α and β estrogen activities and antiandrogen activity in reporter cell lines. Molecular and Cellular Endocrinology, 2002. 193: 43-49.
15. George G. J. M. Kuiper, Eva Enmark, Markku Pelto-Huikko, Stefan Nilsson, Jan-Åke Gustafsson, Cloning of a novel estrogen receptor expressed in rat prostate and ovary. PNAS, 1996. 93: 5925-5930.
16. Gonzalez C, Alonso A, Diaz F, Patterson AM., Dose- and time-dependent effects of 17beta-oestradiol on insulin sensitivity in insulin-dependent tissues of rat: implications of IRS-1. J Endocrinol, 2003. 176: 367-379.
17. Helen B. Hartman, Xiao Hu, Keala X. Tyler, Chiraj K. Dalal, Mitchell A. Lazar, Mechanisms regulating adipocyte expression of resistin. J Biol Chem, 2002. 277: 19754-19761.
18. Jobling, S., Nolan, M., Tyler, C.R., Brighty, G., Sumpter, J.P., Widespread Sexual Disruption in Wild Fish. Environ Sci Technol, 1998. 32: 2498-2506.
19. Jonling, S., D. Sheahan, Inhibition of testicular growth in rainbow trout (Oncorhynchusmykiss) exposed to estrogenic alkylphenoloic chemicals. Environ Toxicol Chem, 1996. 15: 194-202.
20. Kao YH, Hippakka RA, Liao S, Modulation of obesity by a green tea catachin. Am J Clin Nutr, 2000. 72: 1232-1242.
21. Karen J. Ho, James K. Liao, Nonnuclear actions of estrogen. Arterioscler Thromb Vasc Biol., 2002. 22: 1952-1961.
22. Kawanami D, Maemura K, Takeda N, Harada T, Nojiri T, Imai Y, Manabe I, Utsunomiya K, Nagai R., Direct reciprocal effects of resistin and adiponectin on vascular endothelial cells: a new insight into adipocytokine-endothelial cell interactions. Biochem Biophys Res Commun, 2004. 314: 415-419.
23. Kawashima J, Tsuruzoe K, Motoshima H, Shirakami A, Sakai K, Hirashima Y, Toyonaga T, Araki E, Insulin down-regulates resistin mRNA through the synthesis of protein(s) that could accelerate the degradation of resistin mRNA in 3T3-L1 adipocytes. Diabetologia, 2003. 46: 231-240.
24. Kim KB, Seo KW, Kim YJ, Park M, Park CW, Kim PY, Kim JI, Lee SH., Estrogenic effects of phenolic compounds on glucose-6-phosphate dehydrogenase in MCF-7 cells and uterine glutathione peroxidase in rats. Chemosphere, 2003. 50: 1167-1173.
25. Kimmerle R, Heinemann L, Heise T, Bender R, Weyer C, Hirschberger S, Berger M, Influence of continuous combined estradiol-norethisterone acetate preparations on insulin sensitivity in postmenopausal nondiabetic women. Menopause, 1999. 6: 36-42.
26. Kumagai S, Holmang A, Bjorntorp P., The effects of oestrogen and progesterone on insulin sensitivity in female rats. Acta Physiol Scand.7, 13. 149(1): 91-97.
27. Li-Chun Xu, Hong Sun, Jian-Feng Chen, Qian Bian,Jie Qian, Ling Song, Xin-Ru Wang, Evaluation of androgen receptor transcriptional activities of bisphenol A, octylphenol and nonylphenol in vitro. Toxicology, 2005. 216: 197–203.
28. Lindheim SR, Duffy DM, Kojima T, Vijod MA, Stanczyk FZ, Lobo RA., The route of administration influences the effect of estrogen on insulin sensitivity in postmenopausal women. Fertil Steril, 1994. 62: 1176-1180.
29. Louet JF, LeMay C, Mauvais-Jarvis F, Antidiabetic actions of estrogen: insight from human and genetic mouse models. Curr Atheroscler Rep, 2004. 6: 180-185.
30. Maebuchi M, Mavhidori M, Urade R, Ogawa T, Moriyama T, Low resistin levels in adipose tissues and serum in high=fat fed mice and genetically obese mice:development of an ELISA system for quantification of resistin. Arch Biophys, 2003. 416: 164-170.
31. Makoto Iwata, Yuko Eshima, Hiroyuki Kagechika, Hideki Miyaura, The endocrine disruptors nonylphenol and octylphenol exert direct effects on T cells to suppress Th1 development and enhance Th2 development. Immunology Letters, 2004. 94: 135–139.
32. Masami Ishidoa, Masatoshi Moritaa, Syuichi Okab, Yoshinori Masuob, Alteration of gene expression of G protein-coupled receptors in endocrine disruptors-caused hyperactive rats. Regulatory Peptides, 2005. 126: 145– 153.
33. Masuno H, Okamoto S, Iwanami J, Honda K, Shiosaka T, Kidani T, Sakayama K, Yamamoto H., Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells. Toxicol Sci., 2003. 75(2): 314-320.
34. Masuno H, Okamoto S, Iwanami J, Honda K, Shiosaka T, Kidani T, Sakayama K, Yamamoto H., Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells. Toxicol Sci, 2003. 75: 314-320.
35. Mathias F, Johannes K, Susanne N, Markus E, Ralf P., Isoproterenol inhibits resistin gene expression through a Gs protein-coupled pathway in 3T3-L1 adipocytes. FEBS Lett., 2001. 500: 60-63.
36. Moon B, Kwan JJ, Duddy N, Sweeney G, Begum N., Resistin inhibits glucose uptake in L6 cells independently of changes in insulin signaling and GLUT4 translocation. Am J Physiol Endocrinol Metab, 2003. 285: E106-E115.
37. Mori-Abe A, Tsutsumi S, Takahashi K, Toya M, Yoshida M, Du B, Kawagoe J, Nakahara K, Takahashi T, Ohmichi M, Kurachi H, Estrogen and raloxifene induce apoptosis by activating p38 mitogen-activated protein kinase cascade in synthetic vascular smooth muscle cells. J Endocrinol, 2003. 178: 417-426.
38. Naaz A, Zakroczymski M, Heine P, Taylor J, Saunders P, Lubahn D, Cooke PS, Effect of ovariectomy on adipose tissue of mice in the absence of estrogen receptor alpha (ERalpha): a potential role for estrogen receptor beta (ERbeta). Horm Metab Res, 2002. 34: 758-763.
39. Nogueiras R, Gualillo O, Caminos JE, Casanueva FF, Dieguez C, Regulation of resistin by gonadal, thyroid hormone, and nutritional status. Obes Res, 2003. 11: 408-414.
40. Park J, Rho HK, Kim KH, Choe SS, Lee YS, Kim JB., Overexpression of glucose-6-phosphate dehydrogenase is associated with lipid dysregulation and insulin resistance in obesity. Mol Cell Biol, 2005. 25: 5146-5157.
41. Patel L, Buckels AC, Kinghorn IJ, Murdock PR, Holbrook JD, Plumpton C, Macphee CH, Smith SA., Resistin is expressed in human macrophages and directly regulated by PPAR gamma activators. Biochem Biophys Res Commun, 2003. 300: 472-476.
42. Pedersen, S. B., Borglum, J. D., Eriksen, E. F., Richelsen, B., Nuclear estradiol binding in rat adipocytes. Regional variations and regulatory influences of hormones. Biochim Biophys Acta, 1991. 1093: 80-86.
43. Pedersen, S. B., Borglum, J. D., Moller-Pedersen, T., Richelsen, B., Effects of in vivo estrogen treatment on adipose tissue metabolism and nuclear estrogen receptor binding in isolated rat adipocytes. Mol Cell Endocrinol, 1992. 85: 13-19.
44. Pischon T, Bamberger CM, Kratzsch J, Zyriax BC, Algenstaedt P, Boeing H, Windler E., Association of plasma resistin levels with coronary heart disease in women. Obes Res, 2005. 13: 1764-1771.
45. Robert X. Song, Christopher J. Barnes, Zhenguo Zhang, Yongde Bao, Rakesh Kumar, Richard J. Santen, The role of Shc and insulin-like factor 1 receptor in mediating the translocation of estrogen receptor a to the plasma membrane. PNAS, 2004. 101: 2076-2081.
46. S. J. Lazzarini, G. N. Wade, Role of sympathetic nerves in effects of estradiol on rat white adipose tissue. Am J Physiol Regul Integr Comp Physiol, 1991. 260: R47-R51.
47. Sanae Kanno, Seishiro Hirano, Fujio Kayama, Effects of phytoestrogens and environmental estrogens on osteoblastic differentiation in MC3T3-E1 cells. Toxicology, 2004. 196: 137-145.
48. Song H, Shojima N, Sakoda H, Ogihara T, Fujishiro M, Katagiri H, Anai M, Onishi Y, Ono H, Inukai K, Fukushima Y, Kikuchi M, Shimano H, Yamada N, Oka Y, Asano T., Resistin is regulated by C/EBPs, PPARs, and signal-transducing molecules. Biochem Biophys Res Commun, 2002. 299: 291-298.
49. Song IS, Tatebe S, Dai W, Kuo MT., Delayed mechanism for induction of gamma-glutamylcysteine synthetase heavy subunit mRNA stability by oxidative stress involving p38 mitogen-activated protein kinase signaling. J Biol Chem, 2005. 280: 28230-28240.
50. Staples, C.A., J.B. Williams, R.L. Blessing R.T. Varineau., Measuring the biodegradability of nonylphenol ether carbosylates, octylphenol ether carboxylates, and nonylphenol. Chemosphere, 1999. 38: 2029-2039.
51. Stefan Kahlert, Samone Nuedling, Martin van Eickels, Hans Vetter, Rainer Meyer, Christian, Estrogen receptor a rapidly activates the IGF-1 receptor pathway. J Biol Chem, 2000. 275: 18447-18453.
52. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA., The hormone resistin links obesity to diabetes. Nature, 2001. 409: 307-312.
53. T., Gura, Estrogen: key player in heart disease among women. Science, 1995. 269: 771-773.
54. Tabira, Y., M. Nakai, D. Asai, Y. Yakabe, Y. Tahara, T. Shinmyozu, M. Noguchi, M. Takatsuki Y. Shimohigashi, Structural requirements of para-alkylphenols to bind to estrogen receptor. Eur J Biochem, 1999. 262: 240-245.
55. Tanenbaum, D. M., Y. Wang, S.P. Williams and P.B. Sigler, Crystallographic comparison of the estrogen and progesterone receptor's ligand binding domains. PNAS, 1998. 95: 5998-6003.
56. Ulrich Laufs, Oliver Adam, Georg Nickenig, Down-regulation of Rac-1 GTPase by Estrogen. J Biol Chem, 2003. 278: 5956-5962.
57. Utzschneider KM, Carr DB, Tong J, Wallace TM, Hull RL, Zraika S, Xiao Q, Mistry JS, Retzlaff BM, Knopp RH, Kahn SE., Resistin is not associated with insulin sensitivity or the metabolic syndrome in humans. Diabetologia, 2005. 48: 2330-2333.
58. Vendula Kyselova, Jana Peknicova, Daniela BuckiovaandMichael Boubelik, Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice. Reproductive Biology and Endocrinology, 2003: page number not for citation purposes.
59. Verma S, Li SH, Wang CH, fEDAK pwm, Li PK, Wwisel RD, Meisel RD, Mickle DAG, Resistin promotes endithelial cell activation further evidence of adipokine-ecdothelial interaction. Circulation, 2003. 108: 736-740.
60. Wade, G. N., and Gray, J. M., Cytoplasmic 17 beta-[3H]estradiol binding in rat adipose tissues. Endocrinology, 1978. 103: 1695-1701.
61. Xie P, Liu ML, Gu YP, Lu J, Xu X, Zeng WM, Song HP, Oestrogen improves glucose metabolism and insulin signal transduction in HepG2 cells. Clin Exp Pharmacol Physiol, 2003. 30: 643-648.
62. Yang X, Wang W, Fan J, Lal A, Yang D, Cheng H, Gorospe M, Prostaglandin A2-mediated stabilization of p21 mRNA through an ERK-dependent pathway requiring the RNA-binding protein HuR. J Biol Chem, 2004. 279: 49298-49306.
63. Ying, G. G., B. Williams and R. Kookana., Environmental fate of alkylphenols and alkylphenol ethoxylates--a review. Environ. Int., 2002. 28: 215-226.
64. 王正雄, 環境檢驗所, 壬基苯酚環境荷爾蒙對環境生態之影響. 環境檢驗雜誌. 39期.
65. 行政院環境保護署, 國內環境荷爾蒙管理現況. 2003.
66. 凌永健, 清華大學化學系教授, 環境荷爾蒙的化學分析. 環境檢驗雜誌. 32期.
67. 陳晏航, Expression of resistin gene in 3T3-L1 adipocyte is differently regulated by IGF-1,EGCG, and estrogen. 2004.
68. 賴麗瑩, 環保署管考處, 談環境荷爾蒙(上)、(下). 環境檢驗雜誌. 22期.
指導教授 高永旭(Yung-Hsi Kao) 審核日期 2006-1-25
推文 facebook   plurk   twitter   funp   google   live   udn   HD   myshare   reddit   netvibes   friend   youpush   delicious   baidu   
網路書籤 Google bookmarks   del.icio.us   hemidemi   myshare   

若有論文相關問題,請聯絡國立中央大學圖書館推廣服務組 TEL:(03)422-7151轉57407,或E-mail聯絡  - 隱私權政策聲明