以功能性基因體學研究細菌異化辛基苯酚  聚氧乙基醇及抗逆境之基因

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陳啟祥(Chi-Hsiang Chen) 查詢紙本館藏

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以功能性基因體學研究細菌異化辛基苯酚聚氧乙基醇及抗逆境之基因
(Functional genomic study of the genes involved in the bacteria catabolism and stress response to octylphenol polyethoxylates)

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摘要(中)

辛基苯酚聚氧乙基醇 (octylphenol polyethoxylates，OPEOn)為一常用於農業、工業以及一般家庭活動之難分解的非離子性界面活性劑。一旦排放至自然環境時，經常形成辛基苯酸，在結構上類似雌激素，具環境荷爾蒙效力。然而目前對於異化辛基苯酚聚氧乙基醇之酵素的瞭解仍非常有限，本實驗室已分離出可利用辛基苯酚聚氧乙基醇為唯一生長碳源之菌株Pseudomonas nitroreducens TX1，並利用一維及二維膠體電泳分析菌株P. nitroreducens TX1生長於以OPEOn為唯一碳源時所增加或減少表現的蛋白質，為了進一步證明上述發現之增加或減少表現蛋白質的合理性，本研究續以基因體學為研究方法參考全基因體已定序之Pseudomonas sp.，設計引子選殖參與OPEOn代謝及抗逆境反應的相關基因，並以反轉錄聚合酶鏈反應(RT-PCR)觀察其mRNA的表現量是否與一維及二維膠體電泳分析上所發現的蛋白質表現情形相符。本研究計成功地選殖出一個完整序列的基因及七個部份序列的基因，分別包括Arginine / Ornithine transport system substrate-binding proetin、periplasmic binding protein of ATP binding cassette transporter及TolB protein等三個運輸蛋白基因，dihydrolipoamide dehydrogenase所具有之三個與TCA cycle相關的基因群組，一個抗逆境蛋白質superoxide dismutase的基因及一個脂肪酸合成酵素Acetyl-CoA carboxylase carboxyl transferase的基因，另外，反轉錄聚合酶鏈反應的結果顯示，除了Arginine / Ornithine transport system substrate-binding proetin及periplasmic binding protein of ATP binding cassette transporter二個運輸蛋白外，其他蛋白質的基因表現皆與先前在一維及二維膠體電泳上所觀察到的蛋白質表現情形一致，其中，與高分子化合物輸送有關的TolB，其基因在mRNA表現量為選殖運輸蛋白中唯一有表現量增加的蛋白質，至於dihydrolipoamide dehydrogenase基因群組亦全部觀察到有增加表現的現象，初步推測此基因群組除在OPEOn為唯一碳源的表現，除了能增加能量的產生外，dihydrolipoamide dehydrogenase亦能夠利用似Fenton反應產生氫氧自由基，進而藉由氫氧自由基切斷OPEOn的聚氧乙基酸鏈，此外，自由基存在量增加時，本研究進一步發現superoxide dismutase基因的表現同時被誘發，推測除了能抵抗逆境外，文獻彙整顯示尚具有切斷由醇基轉化為羧酸的功能，故其增生表現可能同時會參與OPEOn的代謝。

摘要(英)

The octylphenol polyethoxylates (OPEOn) are recalcitrant non-ionic
surfactants. The release of OPEOn has resulted in concern due to their
metabolites in the environment showing estrogenic activity. However,
little is known about the enzymes involved in the degradation of OPEOn.
A Gram-negative rod, Pseudomonas nitroreducens TX1, was shown to be
able to grow on 0.05-20% (v/v) of OPEOn as sole carbon source. Previous
study also found several proteins to be up-regulated and down-regulated
in response to OPEOn catabolism by 1D- and 2D-electrophoresis analysis.
Consequently, this genomics study was aimed to confirm the expression
of up-regulated and down-regulated proteins from functional proteomics
as mentioned above. The genes in response to OPEOn catabolism and
stress responses were cloned by specific primers, which were designed
from reference Pseudomonas species with whole genome sequencing.
The gene expression was also investigated by RT-PCR to understand if
the expression of mRNA from these genes were consistent to that of
proteins from 1D- and 2D- electrophoresis analysis. One gene with full
length sequence and seven genes with partial sequence have been
successfully cloned in this study. These genes included three transporters
genes (arginine / ornithine transport system substrate-binding protein,
periplasmic binding protein of ATP binding cassette transporter and TolB
protein ), three genes involved in TCA cycle (2-oxoglutarate
dehydrogenase, dihydrolipoamide succinyltransferase and
dihydrolipoamide dehydrogenase), one gene for stress response and one
gene related to fatty acid synthesis. The RT-PCR analysis indicated that
the expression of these genes was consistent to that of proteins by 1D-
and 2D- electrophoresis analysis except that two transporter genes,
arginine / ornithine transport system substrate-binding protein and
periplasmic binding protein of ATP binding cassette transporter. The
expression of transporter genes found to be up-regulated only for
TolB, which is related the transportation of biopolymers. The expression
of dihydrolipoamide dehydrogenase gene clusters was also observed to be
up-regulated. These were suggested to increase the synthesis of energy in
response to OPEOn catabolism. The E3 component of these gene clusters
was further proposed to generate OH radicals by Fenton-like reaction,
which was demonstrated to be able to cleave the polyethoxylates in
OPEOn. Moreover, the expression of superoxide dismutase was also
found to be induced in response to the production of OH radicals. Since
superoxide dismutase has been found to have another function for ether
bond-splitting of carboxylated polyethoxylates from literature survey, the
expression of this enzyme was also suggested to be involved in OPEOn
catabolism.

關鍵字(中)

★ RT-PCR
★ 環境荷爾蒙
★ 辛基苯酚聚氧乙基醇

關鍵字(英)

★ RT-PCR
★ estrogenic activity
★ OPEOn

論文目次

中文摘要…..……………………………………………………………Ⅰ
英文摘要………………………………………………………………..Ⅲ
目錄……………………………………………..………………………Ⅳ
表目錄…………………………………………………………………Ⅸ
圖目錄…………………………………………………………………..Ⅹ
名詞縮寫對照表…………………………………………………..…ⅩⅡ
壹、緒論………………………………………………………………….1
一、界面活性劑的環境荷爾蒙活性…………………………............1
二、菌株Pseudomonas nitroreducens TX1篩選、鑑定與背景.....2
三、P. nitroreducens TX1耗氧酵素的純化與鑑定..............................4
四、P. nitroreducens TX1的功能性蛋白質體學分析..........................4
五、P. nitroreducens TX1的基因體學分析..........................................5
六、研究目的與動機…………………………………………………9
貳、材料及方法…………………………………………………………10
一、培養基與細菌品系………………………………………………10
二、細菌核酸之製備…………………………………………………12
三、利用聚合酶鏈反應(Polymerase Chain Reaction, PCR)做基因選殖…………..……………………………………………………14
四、利用反轉錄聚合酶鏈反應( Reverse Transcription Polymerase Chain Reaction, RT-PCR)觀察………………………………….17
五、生物資訊學的方法分析………………………………………...18
六、實驗儀器與化學藥品…………………………………………...20
叄、結果…………………………………………………………………19
一、P. nitroreducens TX1染色體中的dihydrolipoamide dehydrogenase基因群組........................................................................................22
二、以反轉錄聚合酶鏈反應觀察P. nitroreducens TX1在OPEOn下基因表現........................................................................................27
肆、討論...................................................................................................32
一、菌株P. nitroreducens TX1的dihydrolipoamide dehydrogenase基因族群選殖與功能探討..........................................................34
二、菌株P. nitroreducens TX1的dihydrolipoamide dehydrogenase基因族群啟動探討......................................................................38
三、菌株P. nitroreducens TX1在OPEOn存在下，代謝及抗逆境基因的表現......................................................................................39
伍、結論與建議........................................................................................44
陸、參考文獻............................................................................................46
表..............................................................................................................54
圖..............................................................................................................60
附錄..........................................................................................................89

參考文獻

陸、參考文獻
洪國展. 2004. 分解辛基苯酚聚氧乙基醇之耗氧酵素(二氫硫醯胺脫氫酶)的純化與定性. 國立中央大學生命科學所碩士論文
邱凡峰. 2005.以功能性蛋白質體學研究Pseudomonas nitroreducens TX1生長於辛基苯酚聚氧乙基醇之代謝與逆境反應. 國立中央大學生命科學所碩士論文
徐秉正. 2003. Psedomonas nitroreducens TX1異化辛基苯酚聚氧乙基醇之功能性蛋白質體學：以二維電泳法分析等電點4-8之蛋白質表現. 國立中央大學生命科學所碩士論文
楊嘉蓁. 2001. Triton-100分解菌之分離與分離酵素之特性研究. 國立中央大學生命科學所碩士論文
Benen, J.A. W.J. van Berkel, W.M. van Dongen, F. Muller, and A. De Kok. 1989 Molecular cloning and sequence determination of the lpd gene encoding lipoamide dehydrogenase from Pseudomonas fluorescens. J Gen Microbiol 135:1789-97.
Bentley, S.D., K.F. Chater, A.M. Cerdeno-Tarraga, G.L. Challis, N.R. Thomson, K.D. James, D.E. Harris, M.A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C.W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hornsby, S. Howarth, C.H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O’Neil, E. Rabbinowitsch, M.A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, d. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietzorrek, J. Woodward, B.G. Barrell, j. Parkhill, and D.A. Hopwood. 2002. Complete genome sequence of the model actinomycete Streptomyces codlicolor A3(2). Nature 417:141-7.
Bergey, D. H., J.G. Holt, and N.R. Krieg. 1989. Bergey's manual of systematic bacteriology. Baltimore Williams & Wilkins c1984-c1989.
Blattner, F.R., G. Plunkett, C.A. Bloch, N.T. Perna, V. Burland, M. Riley, J. Collado-Vides, J.D. Glasner, C.K. Rode, G.F. Mayhew, J. Gregor, N.W. Davis , H.A. Kirkpatrick, M.A. Goeden, D. J. Rose, B. Mau, and Y. Shao.1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-74.
Brand, N., G. Mailhot, and M. Bolte. 1998. Degradation photoinduced by Fe(III): method of alkylphenol ethoxylates removal in water. Environ Sci Technol 32:2715-20.
Buell, C. R., V. Joardar, M. Lindeberg, J. Selengut, I. T. Paulsen, M. L. Gwinn, R. J. Dodson, R. T. Deboy, A. S. Durkin, J. F. Kolonay, R. Madupu, S. Daugherty, L. Brinkac, M. J. Beanan, D. H. Haft, W. C. Nelson, T. Davidsen, N. Zafar, L. Zhou, J. Liu, Q. Yuan, H. Khouri, N. Fedorova, B. Tran, D. Russell, K. Berry, T. Utterback, S. E. Van Aken, T. V. Feldblyum, M. D'Ascenzo, W. L. Deng, A. R. Ramos, J. R. Alfano, S. Cartinhour, A. K. Chatterjee, T. P. Delaney, S. G. Lazarowitz, G. B. Martin, D. J. Schneider, X. Tang, C. L. Bender, O. White, C. M. Fraser, and A. Collmer. 2003. The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. Proc Natl Acad Sci U S A 100:10181-6.
Cao, Z., and P. E. Klebba. 2002. Mechanisms of colicin binding and transport through outer membrane porins. Biochimie 84:399-412.
Carothers, D. J., G. Pons, and M. S. Patel. 1989. Dihydrolipoamide dehydrogenase: functional similarities and divergent evolution of the pyridine nucleotide-disulfide oxidoreductases. Arch Biochem Biophys 268:409-25.
Chen, P., M. A. Pickard, and M. R. Gray. 2000. Surfactant inhibition of bacterial growth on solid anthracene. Biodegradation 11:341-7
Cheng, C., N. Kacherovsky, K.M. Dombek, S. Camier, S.K. Thukral, E. Rhim, and E.T. Young. 1994. Identification of potential target genes for Adrlp through characterization of essential uncleotides in UAS1. Mol Cell Biol 14:3842-52.
Cole, S.T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S.V. Gordon , K. Eiglmeier, S. Gas, C.E. Barry, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, A. Krogh, J. McLean , S. Moule, L. Murphy, K. Oliver, J. Osborne, M.A. Quail, M.A. Rajandream , J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J.E. Sulston, K. Taylor, S. Whitehead, and B.G. Barrell. 1998. Deciphering the biology of Myobacterium tuberculosis from the complete genome. Nature 393:537-44.
Dennis, J.J., E.R. Lafontaine, and P.A. Sokol. 1996. Identification and characterization of the tolQRA genes of Pseudomonas aeruginosa. J Bacteriol 178:7059-68.
Fernandes, M., H. Xiao, and J.T. Lis. 1994. Fine structure analyses of the Drosophila and Saccharomyces heat shock factor-heat shock element interactions. Nucleic Acids Res 22:167-73.
Glockner, F.O., M. Kube, M. Bauer, H. Teeling, T. Lombardot, W. Ludwig, D. Gade, A. Beck, K. Borzym, K. Heitmann, R. Rabus, H. Schlesner, R. Amann, and R. Reinhardt. 2003. complete genome sequence of the marine planctomycete Pirellula sp. strain 1. Proc Natl Acad Sci U S A 100:8298-303.
Hassett, D.J., W.A. Woodruff, D.J. Wozniak, M.L. Vasil, M.S. Cohen, and D.E. Ohman. 1993. Cloning and characterization of the Pseudomonas aeruginosa sodA and sodB genes encoding manganese- and iron- cofactored superoxide dismutase: demonstration of increased manganese superoxide dismutase activity in alginate-producing bacteria. J Bacteriol 175:7658-65.
Holden, M.T., R.W. Titball, S.J. Peacock, A.M. Cerdeno-Tarraga, T. Atkins, L. C. Crossman, T. Pitt, C. Churcher, K. Mungall, S.D. Bentley, M. Sebaihia, N.R. Thomson, N. Bason, I.R. Beacham, K. Brooks, K.A. Brown, N.F. Brown, G.L. Challis, I. Cherevach, T. Chillingworth, A. Cronin, B. Crossett, P. Davis, D. DeShazer, T. Feltwell, A. Fraser, Z. Hance, H. Hauser, S. Holroyd, K. Jagels, K.E. Keith, M. Maddison, S. Moule, C. Price, M.A. Quail, E. Rabbinowitsch, K. Rutherford, M. Sanders, M. Simmonds, S. Songsivilai, K. Stevens, S. Tumapa, M. Vesaratchavest, S. Whitehead, C. Yeats, B.G. Barrell, P.C. Oyston, and J. Parkhill. 2004. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A 101:14240-5.
Huang, S. L., C. J. Yang, G. L. Guo, and S. H. Chou. 2004. Isolation, identification and properties of bacterial strains degrading octylphenol polyethoxylates. Taiwan Journal of Agricultural Chemistry and Food Science (In press).
Igarashi, K. and K. Kashiwagi. 1999. Polyamine transport in bacteria and yeast. Biochem J 344:633-42.
Iizuka, H. and K. Komagata. 1964. Microbiological studies on petroleum and natural gas. J Gen Appl Microbiol 10:207-21.
Ikeda, H., J. Ishikawa, A. Hanamoto, M. Shinose, H. Kikuchi, T. Shiba, Y. Sakaki, M. Hattori, and S. Omura. 2003. Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol 21:526-31
Ikebe, T., S. Iyoda, and K. Kutsukake. 1999. Structure and expression of the filA operon of Salmonella typhimurium. Microbiology 145:1389-96.
Ishikawa, J., A. Yamashita, Y. Mikami, Y. Hoshino, H. Kurita, K. Hotta, T. Shiba, and M. Hattori. 2004. The complete genomic sequence of Nocardia farcinica IFM 10152. Proc Natl Acad Sci U S A 101:14925-30.
Isnard, M., A. Rigal, J.C. Lazzaroni, C. Lazdunski, and R. Lloubes. 1994. Maturation and localization of the TolB protein required for colicin import. Journal of Bacteriology 176:6392-6.
Kaneko, T., Y. Nakamura, C.P. Wolk, T. Kuritz, S. Sasamoto, A. Watanabe, M. Iriguchi, A. Ishikawa, K. Kawahima, T. Kimura, Y. Kishida, M. Kohara, M. Matsumoto, A. Matsuno, A. Muraki, N. Nakazaki, S. Shimpo, M. Sugimoto, M. Takazawa, M. Yamada, M. Yasuda, and S. Tabata. 2001. Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 8:227-53.
Kaneko, T., Y. Nakamura, S. Sato, K. Minamisawa, T. Uchiumi, S. Sasamoto, A. Watanabe, K. Idesawa, M. Iriguchi, K. Kawashima, M. Kohara, M. Matsumoto, S. Shimpo, H. Tsuruoka, T. Wada, M. Yamada, and S. Tabata. 2002. Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Res. 9:189-97.
Kunst, F., N. Ogasawara, I. Moszer, A. M. Albertini, G, Alloni, V. Azevedo, M.G. Bertero, P. Bessieres, A. Bolotin, S. Borchert, R. Borriss, L. Boursier, A. Brans, M. Braun, S.C. Brignell, S. Bron, S. Brouillet, C.V. Bruschi, B. Caldwell, V. Capuano, N.M. Carter, S.K. Choi, J.J. Codani, I.F. Connerton, and A. Danchin. 1997. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390:249-56.
Lee, Y.H., T.S. Chang, H.J. Liu, and W.S. Chu. 1998. An acidic glutary-7-aminocephalosporanic acid acylase from Pseudomonas nitroreducens. Biotechnol Appl Biochem 28:113-8.
Li, S.J. and J.E. Cronan. 1992. The genes encoding the two carboxyltransferase subunits of Escherichia coli Acetyl-CoA carboxylase. Journal of Biological Chemistry 267: 16841-7.
Mattevi, A., G. Obmolova, K.H. Kalk, W.J. van Berkel, and W.G. Hol. 1993. Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties. J Mol Biol 230:1200-15.
Nelson, K. E., C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hilbert, V. A. Martins dos Santos, D. E. Fouts, S. R. Gill, M. Pop, M. Holmes, L. Brinkac, M. Beanan, R. T. DeBoy, S. Daugherty, J. Kolonay, R. Madupu, W. Nelson, O. White, J. Peterson, H. Khouri, I. Hance, P. Chris Lee, E. Holtzapple, D. Scanlan, K. Tran, A. Moazzez, T. Utterback, M. Rizzo, K. Lee, D. Kosack, D. Moestl, H. Wedler, J. Lauber, D. Stjepandic, J. Hoheisel, M. Straetz, S. Heim, C. Kiewitz, J. A. Eisen, K. N. Timmis, A. Dusterhoft, B. Tummler, and C. M. Fraser. 2002. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ Microbiol 4:799-808.
Pullikuth, A.K., and S.S. Gill. 1997. Primary structure of an invertebrate dihydrolipoamide dehydrogenase with phylogenetic relationship to vertebrate and bacterial disulfide oxidoreductases. Gene 200:163-72.
Sauer, K. and A.K. Camper. 2001. Characterization of phenotypic changes in Pseudomonas putida in response to surface-associated growth. J Bacteriol 183:6579-89.
Schuller, C., J.L. Brewster, M.R. Alexander, M.C. Gustin, and H. Ruis. 1994. The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J 13:4382-9.
Sokatch, J.R., V. McCully, J. Gebrosky, and D.J. Sokatch. 1981. Isolation of a specific lipoamide dehydrogenase for a branched-chain keto acid dehydrogenase from Pseudomonas putida. J Bacteriol 148:639-46
Staples, C. A., J. B. Williams, R. L. Blessing, and P. T. Varineau. 1999. Measuring the biodegradability of nonylphenol ether carboxylates, octylphenol ether carboxylates, and nonylphenol. Chemosphere 38:2029-39.
Stover, C. K., X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, M. J. Hickey, F. S. Brinkman, W. O. Hufnagle, D. J. Kowalik, M. Lagrou, R. L. Garber, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L. L. Brody, S. N. Coulter, K. R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G. K. Wong, Z. Wu, I. T. Paulsen, J. Reizer, M. H. Saier, R. E. Hancock, and S. Lory, and M. V. Olson. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406:959-64
Timmis, K.N. 2002. Pseudomonas putida: a cosmopolitan opportunist par excellence. Environ Microbiol 12:779-81.
Toivonen, J.M., M.R. Boocock, and H.T. Jacobs. 1999. Modelling in Escherichia coli of mutation in mitoribosomal protein S12: novel mutant phenotypes of rpsL. Molecular Microbiology 31:1735-46.
Wolter, D.J., E. Smith-Moland, R.V. Goering, N.D. Hanson, and P.D. Lister. 2004. Multidrug resistance associated with mexXY expression in clinical isolates of Pseudomonas aeruginosa from a Texas hospital. Diagnostic Microbiology and Infectious Disease 50:43-50.
Wolter, D.J., N.D. Hanson, and P.D. Lister. 2004. Insertional inactivation of oprD in clinical isolates of Pseudomonas aeruginosa leading to carbapenem resistance. FEMS Microbiology Letter 236:137-43.
Xu, J., M.K. Bjursell, J. Himrod, S. Deng, L.K. Carmichael, H.C. Chiang, L.V. Hooper, and J.I. Gordon. 2003. A genomic view of the human-Bacteroides theaiomicron symbiosis. Science 299:2074-6.
Yamashita, M., A. Tani, and F. Kawai. 2005. A new ether bond-splitting enzyme found in Gram-positive polyethylene glycol 6000-utilizing bacterium, Pseudonocardis sp. strain K1. Appl Microbiol Biotechnol 66:174-9.
Yang, H.S., J.I. Morris, Q. Wang, L.G. Korotchkina, M. Kwon, and M.S. patel. 2001. Human dihydrolipoamide dehydrogenase gene transcription is mdiated by cAMP-response element-like site and TACGAC direct repeat. Inl J Biochem and Cell Biology 33:902-13.
Yang, H.S., M. Johnson, and M.S. Patel. 1999. Roles of an ETs motif and a novel CACGAC direct repeat in transcription of the murine dihydrolipoamide dehydrogenase (Dld) gene. Biochem J 338:667-75.
Yao, J., G. Zhang, Q. Wu, G.Q. Chen, and R. Zhang. 1999. Production of polyhydroxyalkanoates by Pseudomonas nitroreducens. Antonie van Leeuwenhoek 75: 345-9.
Zgurskaya, H. I. and H. Nikaido. 2000. Multidrug resistance mechanisms: drug efflux across two membranes. Mol Microbiol 37:219-25.
Zhang, G., X. Hang, G Phillip, K.P. Ho, and G.Q. Chen. 2001. PCR cloning of type Ⅱ polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains. FEMS Microbiology Letters 198:165-70.

指導教授

黃雪莉(Shir-Ly Huang)

審核日期

2005-7-28

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