||This thesis employed the optical triangulation measurement system (including CMOS image sensor, laser light and MATLAB software) to analyze muscle vibration patterns by using silkworm as an animal model. By using this system, the dynamic change of muscle contraction was quantified with 10 μm precision that is unable to be distinguished with naked eye. Each silkworms were nanoinjected with 207 nl of 20% formalin, 4% acetic acid, 10% formic acid or 10% acetylcholine into the 7th segment and muscle vibration patterns were recorded. The acquired vibration information was then transformed into time-position and frequency-amplitude profile. The results showed that puncture and PBS (pH 6.5) injection did not induce significant contraction in silkworm’’s muscle. The amplitudes of peaks are 0.1115 and 0.1673 respectively in low frequency. Either acetic acid, formic acid or acetylcholine injection induces short-term vibrations, but distinguishable amplitude change from that of puncture and PBS injection. The amplitudes of the peaks for acetic acid, formic acid or acetylcholine injection are 0.2380, 0.2612 and 0.2104 respectively in low frequency. The amplitude for acetic acid is 2.7 pixel, and for formic acid and acetylcholine are 1.5 pixel, same as that puncture and PBS injection. In contrast, formalin injection induces significant changes of waveform in both amplitude and spectrum. The largest amplitude of waveform is 4.5 pixel and peak in 0.8644 in low frequency. In conclusion, these studies demonstrated the applicability of triangulation measurement system for the physiology of silkworm larva. The relation and application between the frequency, the amplitude and pain of animals deserves further investigation.|
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