軟骨細胞在組織工程支架之培養研究

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姓名

陳誌遠(Chih-Yuan Chen) 查詢紙本館藏

畢業系所

機械工程學系

論文名稱

軟骨細胞在組織工程支架之培養研究
(chondrocytes cultured in the tissue engineered scaffold)

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摘要(中)

摘要
組織工程是一個試著結合工程技術及生命科學，來解決人們器官受損或衰竭之後所產生病痛甚至死亡的一個方法。從體外的皮膚、眼角膜到體內的腎臟、肝臟甚至是神經、血管…等，都是目前學者研究的方向。在人體中大部分組織的成分及結構都相當複雜，而軟骨組織由於沒有血管、神經及淋巴系統，且僅由一種細胞所組成，是較為簡單的一種組織，所以是許多學者首先研究的方向。之前的學者模擬軟骨細胞培養於多孔性介質的支架時，並未考慮細胞的運動行為會影響其生長情形。而本文的研究的方向，是考慮細胞受化學趨向性影響，以及隨機運動的方式，來描述細胞生長情形，再利用之前學者的實驗結果，來建立較完整的方程式，以及模擬注流式生物反應器培養細胞的結果。本文亦以實驗方式探討老鼠軟骨細胞(IRC)培養於支架(PLGA50/50)時，以浸置的培養方式了解細胞隨時間的成長情形。由理論預估的結果可得知，軟骨細胞培養在支架時，其運動方式會受化學趨向性及隨機運動影響，而影響生長情形；且使用注流式生物反應器培養組織時，即使在較厚的支架中培養，其細胞密度也可以提昇至與正常的軟骨組織相似。

摘要(英)

Abstract
Tissue engineering is a method which combines engineering technology and life science to repair human’s organ failure or injure. The researches in progress cover a variety of tissue types such as skin, cornea, kidney, liver, nerves, blood vessels etc. Most tissues are very complex in both structure and components. Cartilage, which has no blood, nerves and lymph, is relatively a simple tissue only composed of one kind of cells, so it’s been drawn a lot of research interest. In this paper, we consider cell growth in a porous scaffold affected by chemotaxis and random walk, use previous experiment data in the literature to develop a more complete model, and simulate cells cultured by a perfusion bioreactor. An experiment is also performed to understand the static culture of Immortalized Rat Chondrocyte (IRC) in a polymer scaffold (PLGA50/50). Form theoretical work we know when chondrocytes cultured in the scaffold, their migration will effect growth condition. Using the perfusion bioreactor to culture chondrocytes, cell density can be promoted towards normal cartilage tissues even in thick scaffolds.

關鍵字(中)

★ 組織工程
★ 軟骨組織
★ 支架
★ 注流式生物反應器

關鍵字(英)

★ cartilage
★ tissue engineering
★ perfusion bioreactor
★ scaffold

論文目次

目錄
中文摘要
英文摘要
誌謝
目錄……………………………………………………………………..Ⅰ
表目錄.. ………………………………………………………………...Ⅳ
圖目錄…………………………………………………………………..Ⅴ
符號說明………………………………………………………………..Ⅶ
第一章緒論............................................................................................1
1.1 文獻回顧....................................................................................2
1.2 研究動機....................................................................................4
第二章靜態培養理論模式....................................................................5
2.1 方程式的建構............................................................................5
2.2 統御方程式及邊界條件............................................................7
2.3 無因次化....................................................................................9
2.4 解題方法..................................................................................10
2.5 理論驗証..................................................................................11
2.6 無因次參數分析......................................................................12
2.6.1 的影響........................................................................12
2.6.2 Γ的影響..........................................................................12
2.6.3 Rg與Rd的影響...............................................................13
第三章靜態培養實驗..........................................................................14
3.1 材料..........................................................................................14
3.1.1 支架................................................................................14
3.1.2 細胞................................................................................14
3.2 實驗方法..................................................................................15
3.2.1 支架消毒........................................................................15
3.2.2 種植................................................................................15
3.2.3 培養................................................................................15
3.2.4 蛋白質定量....................................................................16
3.3 實驗結果..................................................................................17
3.3.1 細胞數量測定................................................................17
3.3.2 靜態培養結果................................................................17
3.4 改進方法及結果......................................................................17
3.4.1 支架消毒........................................................................18
3.4.2 種植................................................................................18
3.4.3 種植結果........................................................................19
第四章注流式生物反應器設計與分析..............................................20
4.1 注流式生物反應器設計與製做..............................................20
4.1.1 生物反應器的流力環境................................................20
4.1.2 生物反應器須具有的特性及使用材料........................21
4.2 理論建模..................................................................................22
4.3 理論估算結果..........................................................................24
4.3.1 靜態與注流培養的比較................................................24
4.3.2 不同支架厚度時，注流強度值對細胞數目的影響......25
第五章結論與未來展望......................................................................26
參考文獻………………………………………………………………..28
附錄……………………………………………………………………..33

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指導教授

鍾志昂(Chih-Ang Chung)

審核日期

2005-7-20

推文