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姓名 陳佳柏(Chia-Po Chen)  查詢紙本館藏   畢業系所 機械工程學系
論文名稱 模擬注流式生物反應器之細胞培養研究
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摘要(中) 本文多孔性介質的概念並加入細胞化學趨向與隨機漫步的特性來建立一數學模型,包含了連續方程式、動量守恆方程式、細胞質量守恆方程式以及養份濃度守恆方程式來描述細胞在多孔性支架中的培養情況,並分為靜置式培養模擬與注流式培養模擬。其中靜置式培養是模擬傳統培養皿的培養環境,並與之前學者的實驗數據做比對來驗證數學模型的正確性。注流式培養是模擬支架置於注流式生物反應器中,以注流的方式將培養細胞時所需的養分利用對流的特性帶入支架中,彌補傳統養分擴散的缺點,並以其為模擬系統使得模擬的結果可以定性的描述整個物理現象、建立預測並提供實際培養時的參考。在模擬中顯示,細胞在有注流影響下所得到的平均總量會比靜置式培養來的多,且隨著對流速度的增加而更明顯但最後會趨向一漸進值。細胞所具有的化學趨向移動行為與隨機漫步運動均會影響細胞在培養時的數量上的多寡,且隨著流場注流效果的增加而產生不同的物理現象。
摘要(英) This article uses a porous-medium approach, combining chemotaxis and random walk of the cells, to develop a mathematical model, which comprises the continuity equation, momentum equation, cell conservation equation and nutrient conservation equation to describe the growth of chondrocytes in porous scaffolds. Two cases of simulation are performed: static culture and perfusion culture. We use the results of the simulation of the static culture to compare with the experimental studies reported previously. In the perfusion flow case, we simulate the cell growth in a perfusion bioreactor. The results can qualitatively describe the physical phenomena, set up the prediction and offer the actual cultivated reference. The amount of cells in the perfusion culture is more than in static culture and increase with the velocity of perfusion, and it will tend to a constant as the perfusion is intensified. We find the properties of chemotaxis and random walk will influence the growth of cells, and they may reveal different effects when the perfusing is getting stronger.
關鍵字(中) ★ 軟骨細胞
★ 化學趨向性
★ 細胞遷移
★ 注流
★ 隨機漫步
★ 細胞生長
關鍵字(英) ★ chemotaxis
★ cell growth
★ perfusion
★ migration
★ random walk
★ chondrocytes
論文目次 中央大學碩士論文授權書
中文摘要
英文摘要
誌謝
目錄 Ⅲ
表目錄 Ⅵ
圖目錄 Ⅵ
符號說明 Ⅸ
第一章 緒論 1
1.1 前言 1
1.2 研究背景 2
1.3 研究動機 4
第二章 數學模型 6
2.1 基本假設與多孔介質概念 6
2.2 描述注流式流場之連續方程式及動量方程式 9
2.3 描述養份消耗之擴散方程式 11
2.4 描述細胞生長之擴散方程式 13
2.5 方程式簡化 17
2.6 無因次方程式與無因次參數定義 18
2.7 無因次化邊界條件 21
2.7.1 無注流情況 21
2.7.2 有注流影響 22
第三章 數值方法 24
3.1 Femlab簡介 24
3.2 通式 25
3.3 弱解式 25
3.4 網格設計 26
3.4.1 無注流情況 26
3.4.2 有注流影響 27
3.5 誤差與精確度 27
第四章 模擬結果與分析 29
4.1 無注流情況 29
4.2 注流的影響 30
4.3 系統參數對注流培養的影響 33
4.3.1 的影響 33
4.3.2 的影響 35
第五章 結論與未來展望 37
參考文獻 39
附錄 42
附表 54
附圖 58
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Pazzano, D., Mercier, K.A., Moran, J.M., Fong, S.S., DiBiasio, D.D., Rulfs, J.X., Kohles, S.S., and Bonassar, L.J., 2000. Comparison of Chondrogensis in Static and Perfused Bioreactor Culture. Biotechnol. Prog. 16(5): 893-896.
Shreiber, D.I., Barocas, V.H., and Tranquillo, R.T., 2003. Temporal Variations in Cell Migration and Traction during Fibroblast-Mediated Gel Compaction. Biophysical Journal 84: 4102–4114.
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指導教授 鍾志昂(Chih-Ang Chung) 審核日期 2005-7-19
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