博碩士論文 93224019 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:33 、訪客IP:18.188.241.82
姓名 黃偉哲(Wei-Che Huang)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 水稻CCR4基因之功能分析- 水稻CCR4基因的選殖、定性及表現
(Functional Analysis of The CCR4 Genes in Oryza sativa-Cloning, Characterization and Expression of the Rice CCR4 )
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摘要(中) 從酵母菌到人類都存在著CCR4-NOT複合體,這複合體對於基因表達是個整體的調節者。複合體中的各個蛋白質對於不同層面的mRNA 代謝都有關聯,其中包括transcription initiation、elongation和mRNA degradation。其中一種影響基因表現的調控是控制mRNA 的半衰期。大部分的情況下,調控真核細胞mRNA半衰期的主要路徑是先將poly(A) tail縮短。在酵母菌的研究得知,CCR4蛋白質是CCR4-NOT 複合體中的一部分,目前已經證實在細胞質中具有deadenylation的作用。然而CCR4基因在植物系統中的功能並不清楚,所以本篇論文將進一步探討CCR4基因在水稻中的功能。
首先,透過資料庫比對分析發現有5個和酵母菌CCR4同源性較高的OsCCR4蛋白質,同時也發現皆屬於Mg2+-dependent exonuclease蛋白質家族。並發現在缺糖條件下,5個OsCCR4基因表現量較含糖條件下高,對於含糖條件下的相關基因可能有調控的作用。OsCCR4基因在水稻中分別有其組織特異性,其中OsCCR4-2則對於所有組織都有其重要性。藉由GFP融合OsCCR4-1、OsCCR4-2,發現OsCCR4-1、OsCCR4-2在細胞核及細胞質中皆有表現,所以可能參與了細胞核及細胞質中mRNA的deadenylation。並且成功建立過量表達GFP融合OsCCR4-1及OsCCR4-2的轉殖水稻,之後可進一步分析OsCCR4-1與OsCCR4-2在水稻內的功能。在E. Coli 表達系統中,也成功的表達出soluble的OsCCR4-1、OsCCR4-2重組蛋白質,之後將透過In vitro deadenylase activity分析,確認OsCCR4蛋白質的生化功能。
摘要(英) The CCR4-NOT complex is a global regulator of gene expression that is conserved from yeast to human. The proteins of the complex are involved in several aspects of mRNA metabolism, including transcription initiation and elongation and mRNA degradation. One of the regulations of gene expression is influenced by the control of mRNA turnover. In most cases, the major pathway of mRNA turnover in eukaryotic cells are initiated by shortening of the poly(A) tail. In yeast, the CCR4 (carbohydrate catabolism repression 4) protein, as part of the CCR4-NOT complex, has been show to be responsible for cytoplasmic deadenylation. However, the function of CCR4 in plants is not clear yet. In this research, we will investigate the functions of CCR4 in rice. First, there are five OsCCR4 proteins have high homology with yeast CCR4 which compared with the database. And all belongs to Mg2+-dependent exonuclease protein family. The five OsCCR4 genes have higher expression under sugar starvation, so that they have the possibility to regulate the sugar-related genes. The OsCCR4 genes have their tissue specificity in rice. Besides, OsCCR4-2 plays a important role for all tissues in rice. Through the GFP fused with OsCCR4-1 and OsCCR4-2 separately, we observed that OsCCR4-1 and OsCCR4-2 express both in nucleus and cytoplasm. So the OsCCR4-1 and OsCCR4-2 may participate the mRNA deadenylation in nucleus and cytoplasm. The transgenic rice which overexpressed the GFP fused with OsCCR4-1 and OsCCR4-2 separately were successful established, and will be analyzed for the functions of OsCCR4-1 and OsCCR4-2 in rice subsequently. The soluble recombinant proteins of OsCCR4-1 and OsCCR4-2 were expressed in E. Coli. In vitro assay of the deadenylase activity will be performed to ensure the biochemical function of OsCCR4-1 and OsCCR4-2.
關鍵字(中) ★ 水稻CCR4基因 關鍵字(英) ★ OsCCR4
論文目次 中文摘要 I
Abstract II
縮寫表 III
本文目錄 V
圖目錄 IX
表目錄 X
本文目錄
壹、研究動機與目的 1
貳、前人研究 2
1.基因表現的調控機制 2
2.mRNA 降解 3
2.1.mRNA 降解的生物意義 3
2.2.mRNA 降解的路徑 3
3.去腺嘌呤酶(deadenylase) 5
4.CCR4-NOT complex 7
5.CCR4 8
6.水稻 11
7.植物基因轉殖系統 12
參、實驗材料與方法 14
第一部分 構築質體 14
1.資料庫序列分析 14
1.1.Database 14
1.2.Analysis Tool 14
2.製備cDNA 14
2.1.製備DEPC-treated ddH2O(RNase-free) 溶液 14
2.2.RNA的純化(TRIZOL® reagent,Invitrogen) 14
2.3.去除RNA中DNA(DNA-freeTM,Ambion) 15
2.4.合成cDNA(The SuperScriptTMIII First-Strand Synthesis System for RT-PCR,Invitrogen) 15
3.以PCR分別合成OsCCR4s基因片段 16
3.1.primer的設計 16
3.2.以PCR合成DNA片段 16
3.3.瓊脂膠體回收限制酵素沏過的DNA(使用 Gel/PCR DNA Fragments Extraction Kit DF300,Geneaid) 17
4.接合反應 18
4.1.線性載體DNA的去磷作用(Dephosphorylation of linearized plasmid DNA) 18
4.2.黏頭端載體DNA與插入DNA片段的接合(Ligation of cohesive-ended DNA fragment) 19
4.3.齊頭端DNA片段的接合(Ligation of blunt-ended DNA fragment) 20
5.細菌的轉殖作用 20
5.1.製備E. coli competent cell(JM109 strain,BL21 strain) 20
5.2.細菌的轉殖(transformation) 21
5.3.小量純化細菌質體DNA法 22
Quick Mini-prep. of plasmid 22
High-Speed plasmid Mini Kit PD300, Geneaid 22
5.4.大量純化細菌質體DNA法(Plasmid Midi Kit,Geneaid) 23
6.DNA序列分析 24
第二部分 水稻基因轉殖 24
7.水稻原生質體轉殖方法 25
7.1.水稻未成熟胚(或成熟胚)之癒傷組織(callus)誘導 25
7.2.水稻懸浮培養細胞的誘導 25
7.3.分離水稻原生質體 25
7.4.水稻原生質體轉殖 26
8.洋蔥表皮細胞(onion epidermal cell)利用微粒子投射法(particle bombardment)的轉殖 26
8.1.洋蔥表皮細胞的準備 26
8.2.基因槍設定 27
8.3.金粒子的製備 27
8.4.DNA附著 27
8.5.轉殖植物樣品處理 28
9.水稻細胞利用農桿菌(Agrobacterium)轉殖的方法 28
9.1.製作Agrobacterium competent cell(EHA105 strain) 28
9.2.農桿菌的轉殖方法 29
9.3.農桿菌的生化檢測(Ketolactose test) 29
9.4.水稻的轉殖作用 30
第三部分 水稻轉殖株分析方法 31
10.轉殖株GUS assay 31
11.轉殖水稻RNA分析 31
11.1.植物RNA的抽取 31
11.2.RT-PCR分析植物RNA 31
12.轉殖水稻蛋白質分析 31
12.1.植物蛋白質的抽取 31
12.2.植物蛋白質分析 32
13.基因組DNA PCR 32
13.1.基因組DNA 的抽取與純化 32
13.2.PCR 33
第四部分 蛋白質表達 33
14.利用pET 載體系統表達目標蛋白質 33
14.1.將插入DNA接入pET 載體 33
14.2.細菌的轉殖 33
14.3.蛋白質表達 33
14.4.細胞蛋白質萃取 34
14.5.蛋白質純化(IMAC sample Kit,Pro-Chem) 34
14.6.蛋白質濃縮 34
14.7.蛋白質分析 35
蛋白質電泳(SDS-PAGE) 35
西方墨點法(Western blot analysis) 35
肆、實驗結果 37
1.OsCCR4基因的選殖 37
1.1.資料庫比對分析 37
1.2.OsCCR4基因特性分析 37
1.3.選殖OsCCR4基因 38
2.分析水稻中OsCCR4基因的表現 38
2.1.OsCCR4基因在含糖/缺糖環境下表現情形 38
2.2.OsCCR4基因在水稻組織的表現模式 39
3.分析OsCCR4-1及OsCCR4-2在細胞中的位置 39
3.1.載體的構築 40
3.2.OsCCR4-1及OsCCR4-2在細胞中的位置 40
4.分析OsCCR4-1與OsCCR4-2在水稻內的功能-OsCCR4-1及OsCCR4-2 overexpression 基因轉殖水稻的建立與分析 41
4.1.過量表達GFP融合OsCCR4-1及OsCCR4-2基因轉殖水稻的建立 41
4.1.1.載體的構築 41
4.1.2.利用GUS活性分析與螢光顯微鏡觀察GFP確認成功之T0轉殖癒傷組織 41
4.2.利用螢光顯微鏡觀察再生成功的轉殖植株根部GFP表現情形 42
4.3.過量表達GFP融合OsCCR4-1及OsCCR4-2基因轉殖水稻的分析 42
4.3.1.pAUG-1轉殖植株分析 42
4.3.2.pAU1-G轉殖植株分析 43
4.3.3.pAUG-2轉殖植株分析 43
4.3.4.pAU2-G轉殖植株分析 44
5.OsCCR4-1、OsCCR4-2重組蛋白的表達 45
伍、討論 46
1.OsCCR4基因的選殖 46
2.水稻中OsCCR4基因的表現 46
3.OsCCR4-1與OsCCR4-2在細胞中的位置 47
4.過量表達GFP融合OsCCR4-1及OsCCR4-2基因轉殖水稻的建立與分析 48
5.OsCCR4-1、OsCCR4-2重組蛋白質的表達 50
陸、參考文獻 86
附錄 I 92
附錄 II 108
參考文獻 Albert, T.K., Lemaire, M., van Berkum, N.L., Gentz, R., Collart, M.A., and Timmers, H.T. (2000). Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res 28, 809-817.
Astrom, J., Astrom, A., and Virtanen, A. (1992). Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA. J Biol Chem 267, 18154-18159.
Bai, Y., Salvadore, C., Chiang, Y.C., Collart, M.A., Liu, H.Y., and Denis, C.L. (1999). The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol 19, 6642-6651.
Bevan, M.W., and Chilton, M.D. (1982). T-DNA of the Agrobacterium Ti and Ri plasmids. Annu Rev Genet 16, 357-384.
Boeck, R., Tarun, S., Jr., Rieger, M., Deardorff, J.A., Muller-Auer, S., and Sachs, A.B. (1996). The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity. J Biol Chem 271, 432-438.
Bremer, K.A., Stevens, A., and Schoenberg, D.R. (2003). An endonuclease activity similar to Xenopus PMR1 catalyzes the degradation of normal and nonsense-containing human beta-globin mRNA in erythroid cells. RNA 9, 1157-1167.
Brown, C.E., and Sachs, A.B. (1998). Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation. Mol Cell Biol 18, 6548-6559.
Byers, P.H. (2002). Killing the messenger: new insights into nonsense-mediated mRNA decay. J Clin Invest 109, 3-6.
Cao, D., and Parker, R. (2003). Computational modeling and experimental analysis of nonsense-mediated decay in yeast. Cell 113, 533-545.
Chen, J., Chiang, Y.C., and Denis, C.L. (2002). CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase. EMBO J 21, 1414-1426.
Chilton, M.D., Drummond, M.H., Merio, D.J., Sciaky, D., Montoya, A.L., Gordon, M.P., and Nester, E.W. (1977). Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11, 263-271.
Collart, M.A. (2003). Global control of gene expression in yeast by the Ccr4-Not complex. Gene 313, 1-16.
Coller, J., and Parker, R. (2004). Eukaryotic mRNA decapping. Annu Rev Biochem 73, 861-890.
Crossway, A., Hauptli, H., Houck, C.M., Irvine, J.M., J.V., O., and Perani, L.A. (1986). Micromanipulation techniques in plant biotechnology. BioTechniques 4, 320-334.
Daugeron, M.C., Mauxion, F., and Seraphin, B. (2001). The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation. Nucleic Acids Res 29, 2448-2455.
Dehlin, E., Wormington, M., Korner, C.G., and Wahle, E. (2000). Cap-dependent deadenylation of mRNA. EMBO J 19, 1079-1086.
Deluen, C., James, N., Maillet, L., Molinete, M., Theiler, G., Lemaire, M., Paquet, N., and Collart, M.A. (2002). The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions. Mol Cell Biol 22, 6735-6749.
Denis, C.L. (1984). Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108, 833-844.
Denis, C.L., and Malvar, T. (1990). The CCR4 gene from Saccharomyces cerevisiae is required for both nonfermentative and spt-mediated gene expression. Genetics 124, 283-291.
Denis, C.L., Draper, M.P., Liu, H.Y., Malvar, T., Vallari, R.C., and Cook, W.J. (1994). The yeast CCR4 protein is neither regulated by nor associated with the SPT6 and SPT10 proteins and forms a functionally distinct complex from that of the SNF/SWI transcription factors. Genetics 138, 1005-1013.
Dlakic, M. (2000). Functionally unrelated signalling proteins contain a fold similar to Mg2+-dependent endonucleases. Trends Biochem Sci 25, 272-273.
Draper, M.P., Salvadore, C., and Denis, C.L. (1995). Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex. Mol Cell Biol 15, 3487-3495.
Dreyfus, M., and Regnier, P. (2002). The poly(A) tail of mRNAs: bodyguard in eukaryotes, scavenger in bacteria. Cell 111, 611-613.
Dupressoir, A., Morel, A.P., Barbot, W., Loireau, M.P., Corbo, L., and Heidmann, T. (2001). Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding. BMC Genomics 2, 9.
Dykxhoorn, D.M., Novina, C.D., and Sharp, P.A. (2003). Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol 4, 457-467.
Elbashir SM, L.W., Tuschl T. (2001). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev 15, 188-200.
Frischmeyer, P.A., van Hoof, A., O'Donnell, K., Guerrerio, A.L., Parker, R., and Dietz, H.C. (2002). An mRNA surveillance mechanism that eliminates transcripts lacking termination codons. Science 295, 2258-2261.
Fromm, M.E., Taylor, L.P., and Walbot, V. (1986). Stable transformation of maize after gene transfer by electroporation. Nature 319, 791-793.
Gallie, D.R. (1998). A tale of two termini: a functional interaction between the termini of an mRNA is a prerequisite for efficient translation initiation. Gene 216, 1-11.
Gao, M., Fritz, D.T., Ford, L.P., and Wilusz, J. (2000). Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro. Mol Cell 5, 479-488.
Gorman, M.A., Morera, S., Rothwell, D.G., de La Fortelle, E., Mol, C.D., Tainer, J.A., Hickson, I.D., and Freemont, P.S. (1997). The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites. EMBO J 16, 6548-6558.
Gutierrez, R.A., MacIntosh, G.C., and Green, P.J. (1999). Current perspectives on mRNA stability in plants: multiple levels and mechanisms of control. Trends Plant Sci 4, 429-438.
Herrick, D., Parker, R., and Jacobson, A. (1990). Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol Cell Biol 10, 2269-2284.
Hiei, Y., Komari, T., and Kubo, T. (1997). Transformation of rice mediated by Agrobacterium tumefaciens. Plant Mol Biol 35, 205-218.
Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6, 271-282.
Hsu, C.L., and Stevens, A. (1993). Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure. Mol Cell Biol 13, 4826-4835.
Klein, R.M., Wolf, E.D., Wu, R., and Sanford, J.C. (1992). High-velocity microprojectiles for delivering nucleic acids into living cells. 1987. Biotechnology 24, 384-386.
Korner, C.G., and Wahle, E. (1997). Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease. J Biol Chem 272, 10448-10456.
Krens, F.A., Molendijk, L., Wullens, G.J., and Schilperoort, R.A. (1982). In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature 296, 72-74.
Lenssen, E., Oberholzer, U., Labarre, J., De Virgilio, C., and Collart, M.A. (2002). Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway. Mol Microbiol 43, 1023-1037.
Lewis, B.P., Green, R.E., and Brenner, S.E. (2003). Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans. Proc Natl Acad Sci U S A 100, 189-192.
Liu, H.Y., Chiang, Y.C., Pan, J., Chen, J., Salvadore, C., Audino, D.C., Badarinarayana, V., Palaniswamy, V., Anderson, B., and Denis, C.L. (2001). Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme. J Biol Chem 276, 7541-7548.
Llave, C., Kasschau, K.D., Rector, M.A., and Carrington, J.C. (2002). Endogenous and silencing-associated small RNAs in plants. Plant Cell 14, 1605-1619.
Lowell, J.E., Rudner, D.Z., and Sachs, A.B. (1992). 3'-UTR-dependent deadenylation by the yeast poly(A) nuclease. Genes Dev 6, 2088-2099.
Lyznik, L.A., McGee, J.D., Tung, P.Y., Bennetzen, J.L., and Hodges, T.K. (1991). Homologous recombination between plasmid DNA molecules in maize protoplasts. Mol Gen Genet 230, 209-218.
Maillet, L., Tu, C., Hong, Y.K., Shuster, E.O., and Collart, M.A. (2000). The essential function of Not1 lies within the Ccr4-Not complex. J Mol Biol 303, 131-143.
Malvar, T., Biron, R.W., Kaback, D.B., and Denis, C.L. (1992). The CCR4 protein from Saccharomyces cerevisiae contains a leucine-rich repeat region which is required for its control of ADH2 gene expression. Genetics 132, 951-962.
Maquat, L.E., and Carmichael, G.G. (2001). Quality control of mRNA function. Cell 104, 173-176.
Martinez, J., Ren, Y.G., Nilsson, P., Ehrenberg, M., and Virtanen, A. (2001). The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation. J Biol Chem 276, 27923-27929.
Meyer, S., Temme, C., and Wahle, E. (2004). Messenger RNA turnover in eukaryotes: pathways and enzymes. Crit Rev Biochem Mol Biol 39, 197-216.
Mol, C.D., Izumi, T., Mitra, S., and Tainer, J.A. (2000). DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination. Nature 403, 451-456.
Moore, M.J. (2002). RNA events. No end to nonsense. Science 298, 370-371.
Muhlrad, D., and Parker, R. (1994). Premature translational termination triggers mRNA decapping. Nature 370, 578-581.
Parker, R., and Song, H. (2004). The enzymes and control of eukaryotic mRNA turnover. Nat Struct Mol Biol 11, 121-127.
Reverdatto, S.V., Dutko, J.A., Chekanova, J.A., Hamilton, D.A., and Belostotsky, D.A. (2004). mRNA deadenylation by PARN is essential for embryogenesis in higher plants. RNA 10, 1200-1214.
Sakai, A., Chibazakura, T., Shimizu, Y., and Hishinuma, F. (1992). Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae. Nucleic Acids Res 20, 6227-6233.
Scott, A., Wyatt, S., Tsou, P.L., Robertson, D., and Allen, N.S. (1999). Model system for plant cell biology: GFP imaging in living onion epidermal cells. Biotechniques 26, 1125, 1128-1132.
Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.
Thore, S., Mauxion, F., Seraphin, B., and Suck, D. (2003). X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex. EMBO Rep 4, 1150-1155.
Tijsterman, M., Ketting, R.F., and Plasterk, R.H. (2002). The genetics of RNA silencing. Annu Rev Genet 36, 489-519.
Tourriere, H., Chebli, K., and Tazi, J. (2002). mRNA degradation machines in eukaryotic cells. Biochimie 84, 821-837.
Tucker, M., Staples, R.R., Valencia-Sanchez, M.A., Muhlrad, D., and Parker, R. (2002). Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae. EMBO J 21, 1427-1436.
Tuschl, T., Zamore, P.D., Lehmann, R., Bartel, D.P., and Sharp, P.A. (1999). Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev 13, 3191-3197.
Uchida, N., Hoshino, S., and Katada, T. (2004). Identification of a human cytoplasmic poly(A) nuclease complex stimulated by poly(A)-binding protein. J Biol Chem 279, 1383-1391.
van Hoof, A., and Parker, R. (1999). The exosome: a proteasome for RNA? Cell 99, 347-350.
van Hoof, A., Frischmeyer, P.A., Dietz, H.C., and Parker, R. (2002). Exosome-mediated recognition and degradation of mRNAs lacking a termination codon. Science 295, 2262-2264.
Viswanathan, P., Chen, J., Chiang, Y.C., and Denis, C.L. (2003). Identification of multiple RNA features that influence CCR4 deadenylation activity. J Biol Chem 278, 14949-14955.
Yamashita, A., Chang, T.C., Yamashita, Y., Zhu, W., Zhong, Z., Chen, C.Y., and Shyu, A.B. (2005). Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover. Nat Struct Mol Biol 12, 1054-1063.
Yang, Y., Peng, H., Huang, H., Wu, J., Jia, S., Huang, D. and Lu, T. (2004). Large-scale production of enhancer trapping lines for rice functional genomics. Plant Science 167, 281-288.
Zamore, P.D., Tuschl, T., Sharp, P.A., and Bartel, D.P. (2000). RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101, 25-33.
指導教授 陸重安(Chung-An Lu) 審核日期 2006-9-20
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