博碩士論文 93226038 詳細資訊




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姓名 吳建宏(Jian-Hung Wu)  查詢紙本館藏   畢業系所 光電科學與工程學系
論文名稱 光學式生化反應即時偵測系統
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摘要(中) 本文研製一新型光學生物感測器:導模共振(guided mode resonance, GMR)光學生物感測器。本元件利用半導體技術,製作一次波長光柵結構,並利用其產生的窄帶高反射率之效果,及其光柵表面區域之高敏感度,應用於共種生化反應,本元件具有以下優點:免標定、微小化、可高產量、即時偵測、易與其它半導體元件接合之優點。
本研究已將GMR元件組裝至流體系統內,形成GMR流體晶片。除了方便將整個生化實驗於晶片內進行外,所使用到的檢體量也相對的減少,並發展出一套即時檢測平台,縮短了檢測所需時間。目前於晶片本身已能檢測出折射率變化量小於0.001,且偵側去氧核醣核酸之雜交行為,除了可即時觀察出有無雜交反應外,其有無雜交差異量更達9倍之多。對於胜肽合成,能成功偵測出每段長度約0.3nm之胺基酸接合反應及其反應之分子動力學行為。
摘要(英) We report a novel method for detecting biomolecular interaction which utilizes the guided-mode resonance (GMR) phenomenon with narrow-band high reflection efficiency characteristics. The structure of GMR device generally consists of two stages — upper grating layer and waveguide layer. GMR device and flow cell are combined to from a GMR flow cell system. The system has several advantages such as label-free, minimizing, real time monitoring, high throughput, low cost, and the potential to become portable. We show the system experimentally with the ability to detect the refractive index variations <0.001,the amino acid thickness variations <0.3nm. It also can detect DNA hybridization and measure molecular interaction.
關鍵字(中) ★ 光學感測器
★ 導膜共振
關鍵字(英) ★ Biosensor
★ Guide-mode resonance
★ DNA hybridization
論文目次 第一章 緒論......................................................................1
1.1 生物晶片簡介..............................................................3
1.1.1 微陣列晶片...............................................................4
1.1.2 實驗室晶片...............................................................5
1.2 生物反應訊號之光學檢測方法..................................6
1.2.1 螢光共振能量轉移法...............................................7
1.2.2 表面電漿共振儀.......................................................9
1.2.3 MACH-ZEHNDER干涉儀生物感測法......................10
1.2.4全內反射螢光法......................................................11
1.3 研究動機.... ............................................................ 14
1.3.1 GMR發展................................................................15
1.3.2 GMR生物檢測流體晶片....................................... 17
第二章 GMR流體晶片之設計與模擬.............................19
2.1 GMR理論...................................................................19
2.1.1 GMR簡介................................................................19
2.1.2 GMR嚴格耦合波概論............................................20
2.2 GMR流體晶片之設計...............................................26
2.2.1 GMR元件設計........................................................26
2.2.2 GMR流道系統之設計............................................29
2.3 GMR流體晶片之模擬...............................................39
2.3.1 GMR元件之模擬....................................................40
2.3.2 GMR流體晶片之模擬............................................44
第三章 GMR流體晶片之製作與量測.............................48
3.1 GMR流體晶片之製作...............................................48
3.1.1 GMR元件之製作....................................................48
3.1.2 GMR流道之製作....................................................51
3.2 GMR流體晶片之特性量測.......................................54
3.2.1量測系統..................................................................54
3.2.2 GMR流體晶片之特性量測...................................55
3.3 即時監測軟體之開發............................................... 57
3.3.1多項式的曲線擬合..................................................59
3.3.2 系統操作說明.........................................................61
第四章 GMR即時生物檢測流體晶片.............................63
4.1 靈敏度之分析……………………………………….63
4.1.1不同溶液之偵測…………………………………...63
4.1.2 不同濃度已醇溶液偵測..................................................65
4.2 去氧核醣核酸之雜交偵測.................................................67
4.2.1 去氧核醣核酸之雜交......................................................70
4.2.2 DNA雜交實驗流程..........................................................71
4.2.3 去氧核醣核酸偵測訊號分析..........................................76
4.2.4 DNA雜交之重複性偵測..................................................77
4.3 胜肽合成.............................................................................81
4.3.1 胺基酸接合......................................................................82
4.3.2 胜肽合成實驗流程..........................................................86
4.3.3去氧核醣核酸偵測訊.......................................................88
4.3.4胜肽合成之反應動力曲線分析.......................................91
第五章 結論與未來展望............................................................. 93
參 考 文 獻........................................................................... 96
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指導教授 楊宗勳(Tsung-Hsun Yang) 審核日期 2006-7-20
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