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姓名 林孟謙(Mon-Chien Lin)  查詢紙本館藏   畢業系所 物理學系
論文名稱 光照引起的退化對薑黃素吸附脂膜的影響
(Effect of light-inducing degradation on curcumin binding to membrane)
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摘要(中) 本文研究薑黃素(curcumin)與不飽和鏈脂膜之作用,及光照對薑黃素吸附不飽和鏈脂膜的影響。利用isothermal titration calorimetry (ITC)測量薑黃素吸附不飽和鏈脂膜的自由能、焓及熵,以熱力學探討吸附過程。
實驗結果顯示薑黃素吸附不飽和鏈脂膜為放熱反應,且反應為自然發生,不需任何外力既可發生。此外,發現薑黃素經光照後水解的產物不僅無吸附熱產生,亦不影響薑黃素吸附脂膜的過程。
摘要(英) This thesis is to study the binding of curcumin to unsaturated lipid membrane, and the effect of light-inducing degradation on curcumin binding to membrane. We use isothermal titration calorimetry (ITC) to measure the binding free energy, the binding enthalpy and the binding entropy for understanding the thermodynamics of binding process.
The result reveals that, the curcumin binding to the unsaturated lipid membrane is an exothermic reaction, and without any external force , it reacts spontaneously. Moreover, we found that the curcumin’’s result of hydrolysis has neither binding heat nor effect on the process of intact curcumin binding to membrane.
關鍵字(中) ★ 薑黃素
★ 脂質微胞
關鍵字(英) ★ curcumin
★ LUV
★ DOPC
論文目次 第一章 簡介
1-1 生物細胞膜 ••••••••••••••••••1
1-2 薑黃與薑黃素 •••••••••••••••••3
1-3 研究動機 •••••••••••••••••••5
第二章 實驗樣本備製
2-1 實驗樣本 •••••••••••••••••••7
2-2 curcumin 溶液製備 •••••••••••••••7
2-3 脂質微胞製備 •••••••••••••••••12
第三章 實驗
3-1 吸收度-測量curcumin濃度 •••••••••••15
3-2 Isothermal Titration Calorimetry 滴定實驗
-測量curcumin對脂膜吸附的能力 •••••••••••20
第四章 總結 ••••••••••••••••••••33
參考文獻 •••••••••••••••••••••34
附錄一 ••••••••••••••••••••••37
附錄二 ••••••••••••••••••••••38
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[2] M. T. Huang, R. C. Smart, C. Q. Wong, A. H. Conney (1988) Inhibitory effect of curcumin, chlorogenic acid, caffeic acid, and ferulic acid on tumor promotion in mouse skin by 12-O-Tetradecanoylphorbol-13-acetate. Cancer Res. 48:5941-5946.
[3] K. Kohli, J. Ali, M. J. Ansari, Z. Raheman (2005) Curcumin: A natural antiinflammatory agent. Indian J Pharmacol. 37:141-149.
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[5] M. Mall, K. Kunzelmann (2005) Correction of the CF defect by curcumin: hypes and disappointments. Bioessays 27:9-13.
[6] Helgi I. Ingolfsson, Roger E. Koeppe II, Olaf S. Andersen (2007) Curcumin is a modulator of bilayer material properties. Biochemistry 46:10384-10391.
[7] E. Jaruga, A. Sokal, S. Chrul, G. Bartosz (1998) Apoptosis-independent alterations in membrane dynamics induced by curcumin. Exp Cell Res 245:303-312.
[8] M. Khajavi, K. Shiga, W. Wiszniewski, F. He, C. A. Shaw, J. Yan, T. G. Wensel, G. J. Snipes, J. R. Lupski (2007) Oral curcumin mitigates the clinical and neuropathologic phenotype of the Trembler-J mouse: a potential therapy for inherited neuropathy. Am J Hum Genet 81:438-453.
[9] H. H. Tonnesen, J. Karlsen, G. B. van Henegouwen (1986) Studies on curcumin and curcuminoids:VIII. Photochemical stability of curcumin. Z. Lebensm. Unters. Forsch. 183:116-122.
[10] S. Oetari, M. Sudibyo, Jan N. M. Commandeur, R. Samhoedi, Nico P. E. Vermeulen (1996) Effect of curcumin on cytochrome P450 and glutathione S-Transferase activities in rat liver. Biochemical Pharmacology 51:39-45.
[11] N. K. Subbarao, R. I. MacDonald, K. Takeshita, R. C. MacDonald (1991) Characteristics of spectrin-induced leakage of extruded, phosphatidylserine vesicles. Biochim. Biophys. Acta. 1063:147-154.
[12] R. C. MacDonald, R. I. MacDonald, B. P. Menco, K. Takeshita, N. K. Subbarao, L. R. Hu (1991) Small-volume extrusion apparatus for preparation of large unilamellar vesicles. Biochim. Biophys. Acta. 1061:297-303.
[13] Y. J. Wang, M. H. Pan, A. L. Cheng, L. I. Lin, Y. S. Ho, C. Y. Hsieh, J. K. Lin (1997) Stability of curcumin in buffer solutions and characterization of its degradation of its degradation products. J. Pharm. Biomed. Anal. 15:1867-1876.
[14] H. H. Tonnesen, J. Karlsen (1985) Studies on curcumin and curcuminoids:V. Alkaline degradation of curcumin. Z. Lebensm. Unters. Forsch. 180:132-134.
[15] L. C. Price, R. W. Buescher (1997) Kinetics of alkaline degradation of the food pigments curcumin and curcuminoids. J. Food Sci. 62(2):267-269.
指導教授 陳方玉(Fang-Yu Chen) 審核日期 2007-12-28
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