博碩士論文 942204015 詳細資訊




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論文名稱 銅綠微囊藻毒素釋放之研究
(Studies on toxin releasing of Microcystis aeruginosa)
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摘要(中) 銅綠微囊藻屬於藍綠藻(又稱藍綠菌)的一種,其水華特性及所產生具肝臟毒性的微囊藻毒均被廣泛地研究,一般認為微囊藻毒通常存在細胞內,細胞老化及死亡後分解才有釋放現象,然本研究發現過去實驗室所單離出的七株產毒微囊藻株中,其中M.TY-2、M.TN-3、M.TN-4這三株藻在對數成長期期間有釋放毒素到細胞外並且持續在培養基中累積的現象,而另外M.TN-2、M.CY-1、M.KS-15、M.KS-29 四株藻則沒有明顯的藻毒釋放現象,這些產毒的藻株裡,對毒素的釋放似乎存在不同程度的表現。由這些藻株裡,利用PCR放大及轉殖技術分析了產毒微囊藻生合成酵素基因組 (mcyA~J) 中包含ABC transporter ATPase(356th-469th)的 mcyH序列,經軟體操作轉成胺基酸序列後與實驗室前所分析的M.TY-1藻株及基因庫中的PCC7806進行序列比對,發現不同藻株間雖然存在數個不同的差異位置,但彼此間相似程度很高,而在ATPase序列中會影響主動運輸的活性區域胺基酸序列皆相同,僅在其他第389個胺基酸(離胺酸)及第427個胺基酸(麩胺酸)存在變異,然而這樣的變異,並無法歸納出其與毒素的有無釋放有關,而從整段McyH蛋白質的其他不同差異,也無法針對毒素釋放顯著差異的兩群藻株做出區分,此證明了微囊藻毒不同的釋放現象與McyH序列上的差異並無關係。
摘要(英) Microcystis aeruginosa, which is a well known blue-green alga (cyanobacterium), has been extensively studied for its bloom-forming characters and productions of the hepatotoxic microcystins. Microcystins are generally considered remaining within the cells and then releasing are generally considered to be the result of cell is integration due to stress and death. From our studies on the culture of M. aeruginosa clones, we found the toxins were accumulating in the cell-free medium during the log phase of growth in the clones of M.TY-2, M.TN-3 and M.TN-4, while it is absence in the culture of M.TN-2, M.CY-1, M.KS-15 and M.KS-29. Our toxin analysis in the medium showed two groups of clones that release toxin during the log phase or not. In these clones of M. aeruginosa, the sequence of McyH, which is one of microcystin synthetase (mcyA~J), was studied through PCR amplification and transgenic cloning, and then translated into amino acid sequences. Amino acid sequences of these clones were compared with that of previously studied M.TY-1 clone and PCC7806 in Genebank. It was found that the amino acid sequences, especially of McyH in these toxic clones of M. aeruginosa were highly conserved, except at few amino acid sites. From the results, we found that only two amino acid sites, 389th (glutamic acid) and 427th(lysine)amino acid, have mutation among the studied toxic M. aeruginosa clones. However, such mutations could not lead to the toxin releasing difference. By comparison of the amino acid sequence alignment of the entire McyH, the difference of the alignment was found to be no correlation to the toxin releasing ability.
關鍵字(中) ★ 毒素釋放
★ 微囊藻
★ 藍綠藻
★ 微囊藻毒
★ 產毒基因
關鍵字(英) ★ toxin release
★ microcystin
★ McyH
★ microcystis
★ cyanobacteria
論文目次 摘 要....................................................................................................................ii
Abstract.............................................................................................................. iii
誌 謝................................................................................................................... v
目錄.................................................................................................................... vi
圖目錄.................................................................................................................vii
表目錄................................................................................................................viii
第一章、緒言...................................................................................................... 1
1-1 研究動機............................................................................................... 1
1-2 研究目的............................................................................................... 3
第二章、文獻回顧............................................................................................... 4
2-1 藍綠藻及藍綠藻毒素............................................................................ 4
2-2 微囊藻與微囊藻毒................................................................................ 7
2-3 國內外微囊藻的危害.......................................................................... 10
2-4 微囊藻毒的生合成基因研究............................................................... 12
2-5 ABC 轉運蛋白 (ABC transporter) .................................................... 14
2-6 微囊藻毒的生合成與釋放................................................................... 15
第三章、材料與方法......................................................................................... 17
3-1 微囊藻株的來源與藻細胞的培養........................................................ 17
3-2 藻細胞計數......................................................................................... 19
3-3 細胞內微囊藻毒萃取.......................................................................... 19
3-4 細胞外微囊藻毒萃取.......................................................................... 20
3-5 微囊藻毒高效液相層析....................................................................... 20
3-6 藻細胞DNA 提取與mcyH 基因序列分析......................................... 21
第四章、結果.................................................................................................... 26
4-1 不同微囊藻株細胞內外毒素的分析.................................................. 26
4-2. 微囊藻毒生合成酵素之一段McyH 蛋白質序列比對....................... 37
第五章、討論.................................................................................................... 42
參考文獻............................................................................................................ 49
參考文獻 陳逸民,1996。台灣本地數種微囊藻(Microcystis)之微囊藻毒成分分析與結構鑑定。國立台灣大學漁業科學研究所碩士論文。
劉志仁,1996。水庫優養化對水質影響及水質處理研究 : 優養化水中藻類細胞及其外代謝物之潛在危害性及去除。國立台灣大學環境工程研究所碩士論文。
李宗徽,1998。臺灣銅綠微囊藻株所含微囊藻毒之毒性分析與化學結構鑑定。國立台灣大學動物學研究所博士論文。
陳逸民,2004。微囊藻毒檢測方法與毒理機制的探討。國立台灣大學海洋研究所博士論文。
林健智,2007。微囊藻產毒基因檢測開發技術與微囊藻毒素釋放生理研究。國立台灣大學漁業科學研究所碩士論文。
行政院環保署, 2005。中華民國環境保護統計年報,pp.58-67。
溫清光,2005。以生態工法去除水庫集水區營養源研究計畫。
Anderson, R. J., Luu, H. A., Chen, D. X., Holmes, C. F., Kent, M. L., Blanc, M. L., Taylor, F. J. R., and Williams, D. E., 1993. Chemical and biological evidence links microcystins to salmon ‘Netpen liver disease’. Toxicon, 31, 1315-1323.
Batista, T., de Sousa G., Suput, J. S., Rahmani, R., and Suput, D., 2003. Microcystin-LR causes the collapse of actin filaments in primaryhuman hepatocytes. Aquat. Toxicol., 65, 85-91.
Böhm, A., Diez, J., Diederichs, K., Welte, W., and Boos, W., 2002. Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli: implications for mal gene regulation, inducer exclusion, and subunit assembly. J .Biol. Chem., 27, 3708-17.
Briand, J. F., Jacquet, S., Bernard, C., and Humbert, J.F., 2003. Health hazards for terrestrial vertebrates from toxic cyanobacteria in surface water ecosystems. Vet. Res., 34, 361-77
Carmichael, W. W., Azevedo, S. M., An, J. S., Molica, R. J. R., Jochimsen, E. M., Lau, S., Rinehart, K. L., Shaw, G. R., and Eaglesham, G. K., 2001. Human fatalities from cyanobacteria: chemical and biological evidence for cyanotoxins. Environ. Health Perspect., 109, 663-668.
Carmichael, W. W., Beasely, V., Bunner, D. L., Eloff, J. N., Falconer, I., Gorham, P., Harada, K. I., Krishnamurthy, T., Yu M. J., Moore, R. E., Rinehart, K., Runnegar, M., Skulberg, O. M., and Watanabe, M., 1988. Naming of cyclic heptapeptide toxins of cyanobacteria (blue-green algae). Toxicon, 26, 971-973.
Carmichael, W. W., Eschedor, J. T., Patterson, G. M. L., and Moore, R. E., 1988. Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend L575 from New Zealand. Appl. Environ. Microbiol., 54, 2257-2263.
Carmichael, W. W., Evans, W. R., Yin, Q. Q., Bell, P., and Moczydlowski, E., 1997. Evidence for paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei (Farlow ex Gomont) comb. nov. Appl. Environ. Microbiol., 63, 3104-10.
Carmichael, W. W., He, J. W., Eschedor, J., He Z. R., and Juan, Y. M., 1994. Partial structural determination of hepatotoxic peptides from Microcystis aeruginosa (cyanobacterium) collected in ponds of central China. Toxicon, 26, 1213-1217.
Chen, Y. M., Lee, T. H., Huang, R., Bai, J. Y., and Chou, H. N., 2006. Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins. Toxicon, 47, 742-746.
Chorus, I., and Bartram, J., 1999, Toxic Cyanobacteria in Water: a guide to their public health consequences, monitoring and management. St. Edmundsbury Press, London, 432p., .
Chorus, I., 2001, Cyanotoxin occurrence in freshwaters—A summary of surveyresults from different countries. In Cyanotoxins— Occurrence, Causes, Consequences, Chorus, I. (Ed.).. Berlin, New York. pp. 75–78.
Codd, G. A., 1984. Toxins of freshwater cyanobacteria. Microbiol. Sci., 1, 48-52.
Codd, G. A., Morrison, L.F., and Metcalf, J.S., 2005. Cyanobacterial toxins: risk management for health protection. Toxicol. Appl. Pharmacol., 203, 264– 272.
Dittmann, E., and Börner, T., 2005. Genetic contributions to the risk assessment of microcystin in the environment. Toxicol. Appl. Pharmacol., 203, 192-200
Dittman, E., Meiβner, K., and Börner, T., 1996. Conserved sequences of peptide synthetase genes in the cyanobacterium Microcystis aeruginosa. Phycologia, 35, 62-67.
Dittmann, E., Neilan, B. A., Erhard, M., Von Döhren, H., and Börner, T., 1997. Insertional mutagenesis of a peptide synthetase gene which is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Mol. Microbiol., 26, 779-787
Falconer, I. R. 1999. An overview of problems caused by toxic bluegreen algae (cyanobacteria) in drinking and recreational water. Environ. Toxicol., 14, 5–12.
Falconer, I. R., Dornbusch, M., Moran, G., and Yeung, S. K., 1992. Effect of the cyanobacterial (blue-green algal) toxins from Microcystis aeruginosa on isolated enterocytes from the chicken small intestine. Toxicon, 30, 790-793.
Fastner, J., Erhard M., and Von Döhren H., 2001. Determination of oligopeptide diversity within a natural population of Microcystis spp. (Cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environm. Microbiol., 67, 5069-5076.
Fujiki, H., and Sugnuma, M., 1999. Unique features of the okadaic acid activity class of tumor promoters. J. Cancer Res. Clin. Oncol., 125, 150-155.
Gaedeke, N., Klein, M., Kolukisaoglu, U., Forestier, C., Müller, A., Ansorge, M., Becker, D., Mamnun, Y., Kuchler, K., Schulz, B., Mueller-Roeber, B., and Martinoia, E., 2001. The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement. EMBO J., 20, 1875-87.
Gaete, V., Canelo, E., Lagos, N., and Zambrano, F., 1994. Inhibitory effects of Microcystis aeruginosa toxin on ion pumps of the gill of freshwater fish. Toxicon, 32, 121-127.
Gentien, P., and Arzul, G., 1990. Exotoxin production by Gyrodinium cf. aureolum (Dinophyceae). J. Mar. Biol. Asso. U. K., 70, 571–581.
Goodman, C. D., Casati, P., and Walbot, V., 2004. A multidrug resistance-associated protein involved in anthocyanin transport in Zea mays. Plant Cell., 18, 12-26.
Gunter, S., Anke, S., Sabine, H., Heidi, L., and Erwin, S., 1999. Functional consequences of mutations in the conserved`signature sequence' of the ATP-binding-cassette protein MalK. Eur. J. Biochem., 266, 420-430
Haider, S., Naithani, V., Viswanathan, P. N., and Kakkar, P., 2003. Cyanobacterial toxins: a growing environmental concern, Chemosphere, 52, 1-21.
Hans, U., and Nina, G., 1992. Toxin production by microcystis aeruginosa as a function of light in continuous cultures and its ecological significance. Appl. Environ. Microbiol., 58, 1321-1325
Harada, K., 2004. Production of secondary metabolites by freshwater cyanobacteria. Chem. Pharm. Bull. (Tokyo), 52, 889-99.
Henatsch, J. J., and Juttner, F., 1983. Volatile odorous excretion products of different strains of Synechococcus (Cyanobacteria). Water Sci. Technol., 15, 259-266.
Higgins, C. F., 1992. ABC transporter from microorganisms to man. Annu. Rev. Cell Biol., 8, 67-113.
Higgins, C. F., 2001. ABC transporters: physiology, structure and mechanism. Res. Microbiol., 152, 205–210.
Hitzfeld, B. C, Hoger, S. J., and Dietrich, D. R., 2000. Cyanobacterial toxins: removal during drinking water treatment, and human risk assessment. Environ. Health. Perspect., 108, 113-22.
Huges, E. O., Gorham P. R., and Zehnder A., 1958. Toxicity of a unialgal culture of Microcystis aeruginosa. J. Microbiol., 4, 225-236.
Jewel, M. A. S., Affan, M. A., and Khan, S., 2003. Fish mortality due to cyanobacterial bloom in an aquaculture pond in Bangladesh. Pakistan J. Biol. Sci., 6, 1046–1050.
Kaya, K., 1996. Toxicology of miccrocystins. In: Toxic Microcystis. Watanabe M. F., Harada K. I., Carmichael W. W. and Fujiki H. (Eds). CRC Press, New York, pp. 175-202.
Kaebernick, M., Dittmann, E., Börner, T., and Neilan, B. A., 2002. Appl. Environ. Microbiol., 68, 449-455.
Kaebernick, M., Neilan, B. A., Börner.T., and Dittmann, E., 2000. Light and the transcriptional response of the microcystin biosynthesis gene cluster. Appl. Environ. Microbiol., 66, 3387–3392.
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., and Kimura, T., 1996. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions (supplement). DNA Res., 3,185-209.
Kleinkauf, H., and von Döhren H., 1996. A non-ribosomal system of peptide biosynthesis. Eur. J. Biochem., 236, 335-351.
Kondratieva, E. N., Pfennig, N., and Truper, H. G., 1992. Thephototrophic prokaryotes. Prokaryotes, 312–330.
Kostakioti, M., Newman, C. L., Thanassi, D. G., and Stathopoulos, C., 2005. Mechanisms of protein export across the bacterial outer membrane.
J. Bacteriol., 187, 4306-14.
Krishnamurthy, T., Carmichael, W. W., and Sarver, E. W., 1986. Toxic peptides from freshwater cyanobacteria (Blue-green algae): Isolation, purification and characterization of peptides from Microcystis aeruginosa and Anabaena flos-aquae. Toxicon, 24, 865-873.
Krishnamurthy, T., Szafraniec, L., Hunt, D. F., Shabanowitz, J., Yates III JR, Hauer, C. R., Carmichael, W. W., Skulberg, O., Codd, G. A., and Missler, S., 1989. Structural characterization of toxic cyclic peptides from blue-green algae by tandem mass spectrometry. Proc. Natl. Acad. Sci. USA., 86, 770-774.
Lawton, L. A., Campbell, D. L., Beattie, K. A., and Codd, G. A., 1990. Use of a rapid bioluminescence assay for detecting cyanobacterial microcystin toxicity. Lett. Appl. Microbiol., 11, 205-207.
Lawton, L. A., and Edwards, C., 2001. Purification of microcystins. J. Chromat. A., 912, 191-209.
Lawton, L., Robertson, P., Cornish, B., and Jaspars, M., 1999. Detoxification of microcystins (cyanobacterial hepatotoxins) using TiO2 photocatalytic oxidation. Environ. Sci. Technol., 33, 771–775.
Lee, T. H., Chen, Y. M., and Chou, H. N., 1998. First report of microcystins in Taiwan. Toxicon, 36, 247-255.
Lee, T. H., Chen, Y. M., and Chou, H. N., 1999. Toxicity assay of cyanobacterial strains using Artemia salina in comparison with the mouse assay. Acta. Zool. Taiwanica., 10, 1-9.
Lynn, J. R., and Rocco, L. M., 2001. Life in extreme environments. Nature, 409, 1092-1101.
Mackintosh, C., Beattie, K. A., Klump, C., Cohen, C., and Codd, G. A., 1990.Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatase 1 and 2A from both animals and higher plants. FEBS Lett., 264, 187-192.
Matsunaga, S., Moore, R. E., Niemczura, W. P., and Carmichael, W. W., 1989. Anatoxin-a(S), a potent anticholinesterase from Anabaena flos-aquae. J. Am. Chem. Soc., 111, 8021–8023.
Mole, J., Chow, C., Drikas, M., and Burch, M., 1997. The influence of culture media on growth and toxin production of the cyanobacterium Microcystis aeruginosa Kütz Emend Elenkin. Paper presented at the13th Annual Conference of the Australian Society for Phycology and Aquatic Botany, Hobart.
Murphy, T., Lawson, A., Nalewajko, C., Murkin, H., Ross, L., Oguma, K., and Mclntyre, T., 2000. Algal toxins: initiators of avian botulism, Environ. Toxicol., 15, 558-567.
National Rivers Authority, 1990. Toxic Blue-Green Algae. Water Quality Series No. 2., National Rivers Authority, London, 128p.
Namikoshi, M., Rinehart, K. L., Sakai, R., Stotts, R. R., Carmichael, W. W., and Evans, W. R., 1992. Identification of 12 hepatotoxins from a Homer Lake bloom of the cyanobacteria Microcystis aeruginosa, Microcystis viridid, and Microcystis wesenbergii: nine new microcystins. J. Org. Chem., 57, 866-872.
Narita, S., Kanamaru, K., Matsuyama, S., and Tokuda, H., 2003. A mutation in the membrane subunit of an ABC transporter LolCDE complex causing outer membrane localization of lipoproteins against their inner membranespecific signals. Mol. Microbiol., 49, 167–177.
Nicholson, B. C., and Shaw, G. R., 2001. Instrumental methods for the determination of cyanobacteria toxins. Proceedings of the AWWA 2001Water Quality Technology Conference, Nashville, Tenn.,11– 15.
Nishizawa, T., Asayama, M., and Shirai, M., 2001. Cyclic heptapeptide microcystin biosynthesis requires the glutamate racemase gene. Microbiology, 147, 1235–1241.
Nishiwaki, R., Ohta, T., Sueoka, E., Suganuma, M., Harada, K., Watanabe, M.F., and Fujiki, H., 1994. Two significant aspects of microcystin-LR: specific binding and liver specificity. Cancer Lett., 83, 283-289.
Nishizawa, T., Ueda, A., Asayama,M., K., and Shirai, M., 2000. Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin. J. Biochem., 127, 779–789
Omata, T., Gohta, S., Takahashi, Y., Harano, Y., and Maeda, S., 2001. Involvement of a CbbR homolog in low CO2-induced activation of the bicarbonate transporter operon in cyanobacteria. J. Bacteriol., 183, 1891-1898.
Omata, T., Price, G. D., Badger, M. R., and Ogawa, T., 1999. Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942. Proc. Natl. Acad. Sci. USA., 9, 13571–13576.
Oudra, B., Loudiki, M., Sbiyyaa, B., Martins, R., Vasconcelos, V., and Namikoshi, N., 2001. Isolation, characterization and quantification of microcystins (heptapeptides hepatotoxins) in Microcystis aeruginosa dominated bloom of Lalla Takerkoust lake-reservoir (Morocco). Toxicon, 39, 1375-81.
Ouyang, Z., and Isaacson R., 2006. Identification and characterization of a novel ABC iron transport system, fit, in Escherichia coli. Infect. Immun., 74, 6949-56.
Pearson, L. A., Hisbergues, M., Börner, T., Dittmann, E., and Neilan, B. A., 2004. Inactivation of an ABC transpoter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC7806. Appl. Environ. Microbiol., 70, 6370-6378.
Pouria, S., de Andrade, A., Barbosa, J., Cavalcanti, R.L., Barreto, V.T., Ward, C.J., Preiser, W., Poon, G.K., Neild, G.H., and Codd, G. A., 1998. Fatal microcystin intoxication in haemodialysis unit in Caruaru, Brazil. Lancet, 352, 21-26.
Robarts, R.D. and Zohary, T., 1987, Temperature effects on photosynthetic capacity, respiration, and growth rates of bloom-forming cyanobacteria. J. Mar. Freshwater Res., 21, 391-399.
Reinkainen, M., Ketola M. and Walls, M., 1994. Effects of the concentrations of toxic Microcystis aeruginosa and an alternative food on the survival of Daphnia pulex. Limnol. Oceanogr., 39, 424-432.
Runnegar, M. T., and Falconer, I. R., 1981. Isolation, characterization and pathology of the toxin from the blue-green alga Microcystis aeruginosa. In: The Water Environment: Algal Toxins and health. Carmichael W. W. (Ed.), New York, 325-342.
Runnegar, M. T., Gerdes, R. G., and Falconer, I. R., 1991. The uptake of thecyanobacterial hepatotoxin microcystin by isolated rat hepatocytes. Toxicon, 29, 43-51.
Saurin, W., Hofnung, M., and Dassa, E., 1999. Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters. J. Mol. Evol., 48, 22–41.
Schneider, E., and Hunke, S., 1998. ATP-binding-cassette (ABC) transport systems: functional and structural aspects of the ATP-hydrolyzing subunits/domains. FEMS Microbiol Rev., 22, 1-20.
Shitan, N., Bazin, I., Dan, K., Obata, K., Kigawa, K., Ueda, K., Sato, F., Forestier, C., and Yazaki, K., 2003. Involvement of CjMDR1, a plant multidrug-resistance-type ATP-binding cassette protein, in alkaloid transport in Coptis japonica. Proc. Natl. Acad. Sci. U.S.A., 100, 751-6.
Silva, E. I., 2003. Emergence of a Microcystis bloom in an urban water body, Kandy Lake., Sri. Lanka. Curr. Sci., 85, 723–725.
Sivonen, K., and Jones, G. 1999. Cyanobacterial toxins. In Toxic cyanobacteria in water: a Guide to Their Public Health Consequences, Monitoring, and Management. NewYork, pp. 41–111.
Stotts, R. R., Namikoshi, M., Haschek, W. M., Rinehart, K. L., Carmichael, W. W., Dahlem, A. M., and Beasley, V. R., 1993. Structural modifications imparting reduced toxicity in microcystins from Microcystis spp. Toxicon, 31, 783-789.
Svrcek, C., and Smith, D. W., 2004. Cyanobacteria toxins and the current state ofknowledge on water treatment options: a review, J. environ. Engine. Sci., 3, 155-185
Wachter, J. K., and Andelman, J. B., 1984. Organohalide formation on chlorination of algal extracellular products environmental science & Technology. J. Environ. Sci. Technol., 18, 811-817.
Wiedner, C., Visser, P. M., Fastner, J., Metcalf, J. S., Codd, G. A., and Mur, L. R., 2003. Effects of light on the microcystin content of Microcystis strain PCC 7806. Appl. Environ. Microbiol., 69, 1475-81.
William, S. S., and Fischer, D. H., 1992. The phosphomannosyl recognition system for intracellular and intercellular transport of lysosomal enzymes. J. Cell. Biochem., 18, 67-85
Woese, C.R., 1987. Bacterial evolution. Microbiol. Rev. 51, 221-271.
Tillett, D., Dittmann, E., Erhard, M., Von Döhren H., Börner, T., and Neilan, B. A., 2000. Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC 7806: an integrated peptide-polyketide synthetase system. Chem. Biol., 7, 753–764.
Toivola, D. M., and Eriksson, J. E., 1999. Toxins affecting cell signaling and alternation of cytoskeletal structure. Toxicol. in vitro., 13, 521-530.
Toivola, D. M., Eriksson, J. E. and Brautigan, D. L., 1994. Identification of protein phosphatase 2A as the primary target for microcystin-LR in rat liver homogenates. FEBS. Lett., 344, 175-180.
Urbatsch, I. L., Beaudet, L., Carrier, I., and Gros, P., 1998. Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites. Biochemistry, 37, 4592-602.
Victor, V.B., and Himadri, B.P., 1995. Molecular identification of an ABC transporter complex for manganese. EMBO J., 14,1845-1853
White, S. H., Duivenvoorden, L. J., and Fabbro, L. D., 2005. A decision-making framework for ecological impacts associated with the accumulation of cyanotoxins (cylindrospermopsin and microcystin). Lake Reserv. Manage., 10, 25–37.
WHO, 1997. Report of the Working Group on Chemical Substances in Drinking Water, Section 5-2 Microcystin-LR. Geneva, Switzerland. pp. 152-172.
WHO, 1998. WHO Guidelines for Drinking-Water Quality: Recommendati ons, Health Criteria and Other Supporting information, 2nd ed. World Health Organization, Geneva, Switzerland. pp. 79-81
WHO, 2003. Algae and cyanobacteria in fresh water. In: Guidelines for Safe Recreational Water Environments. Vol. 1: Coastal and Fresh Waters. World Health Organization, Geneva, Switzerland, pp. 136–158.
Williams, D. E., Dawe, S. C., Kent, M. L., Anderson, R. J., Craig, M., and Holmes, C. F. B., 1997. Bioaccumulation and clearance of microcystins from salt water mussels, Mytilus edulis, and in vivo evidence for covalently bound microcystins in mussel tissues. Toxicon, 35, 1617-1625.
Wittmann, C., and Heinzle, E., 2001. Maldi-tof MS for qualification of substrates and products in cultivations of Corynebacterium glutamicum. Biotech. Bioengin., 72, 642-647.
Xu, L. H., Lam, P. K. S., Chen, J. P., Xu, J. M., Wong, B. S. F., Zhang, Y. Y., Wu, R. S., and Harada, K. I., 2000. Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. Chemosphere, 41, 53-58
Yasuko, I., Matsuzawa, H., Matsuyama, S., Narita, S., and Tokuda ,H., 2006. Genetic analysis of the mode of interplay between an ATPase subunit and membrane subunits of the lipoprotein-releasing ATP-binding cassette transporter LolCDE. J. Bacteriol., 188, 2856-64.
Yen, H. K., Lin, T. F., Tseng, I. C., Tung, S. C., Hsu, M. H., and Liao, P. C., 2004. Occurrence of Algal Toxins and Odorants in Two Reservoirs in South Taiwan, The 10th International Drinking Water Quality Management and TreatmentTechnology, Taipei, Taiwan.
Zurawell, R. W., Chen, H., Burke, J. M., and Prepas, E. E., 2005. Hepatotxic cyanobacteria: a review of the biological importance of microcystins in freshwater environments. J. Toxicol.environ. Health, Part B, 8, 1–37
指導教授 周宏農(Hong-Nong Chou) 審核日期 2007-7-11
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