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姓名 洪國川(Guo-chuan Hong) 查詢紙本館藏 畢業系所 物理學系 論文名稱 共焦拉曼與螢光顯微鏡之發展及其在材料診斷上之應用 相關論文 檔案 [Endnote RIS 格式]
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摘要(中) 共焦顯微鏡利用空間濾波原理，達成點對點共焦成像特性，使得不需要把樣品真的切開，就能觀察到切片影像，將光學顯微術的非侵入性偵測能力發揮得淋漓盡致。共焦顯微鏡搭配螢光顯微技術，可將藥物配合螢光物質注入生物細胞中，追蹤藥物在細胞內的流動路徑；以及利用掃描系統得知螢光物質的空間分佈，以便鎖定單一個螢光物質進行其特性分析。共焦顯微術也可結合拉曼光譜技術，可對材料的鍵結組成有進一步的了解。本論文中，我們架設一台共焦拉曼與螢光顯微系統，之後利用測試樣品來對系統特性進行分析，橫向解析度測試部分，是掃描螢光物質的空間分佈，從其強度分佈的半高全寬來定義系統的橫向解析度，系統橫向解析度為0.6 μm；縱向解析度測試部分，是移動物鏡的前後位置，來得到縱向的反應曲線，一樣從其強度分佈的半高全寬來定義系統的縱向解析度，系統縱向解析度為5.5 μm；拉曼光譜量測部分，是先以石墨、單晶鑽石當作測試樣品，確認系統可量測出拉曼光譜，之後對我們用飛秒級脈衝雷射沉積的特殊結構之碳薄膜進行拉曼光譜量測，並探討特殊結構之碳薄膜的空間分佈及鍵結組成。
摘要(英) Confocal microscopy is a valuable tool in seeing clear images inside thick samples. It is also very suitable for biological researches due to its non-invasive nature. By using fluorescence technology confocal microscopy can trace medication with fluorescence materials in the cell. Using scanning system to obtain spatial distribution of the fluorescence materials, then analyzing the property for a fluorescence material. Also using Raman spectrum technology confocal microscopy to obtain bonding component of the material. In this thesis, we designed and sep up a confocal Raman and fluorescence microscopy. After the system has been installed, we test it performance about resolution. For example, if the wavelength is 637 nm, using an objective with 1.3 numerical aperture, its transverse and axial resolution are 0.6 μm and 5.5 μm, respectively. For Raman spectrum measurement, we used the graphite and single crystal diamond as standard sample to demonstrate our system. Then we measured carbon film which be product with femtosecond laser PLD, and we analyzing spatial distribution and bonding component of special structure of carbon film in the feature.
關鍵字(中) ★ 共焦顯微鏡
★ 拉曼光譜
★ 螢光
★ 脈衝雷射沉積關鍵字(英) ★ confocal microscope
★ fluorescence
★ Raman spectrum
★ pulse laser deposition論文目次 中文摘要………………………………………………………………I
英文摘要………………………………………………………………II
致謝……………………………………………………………………III
目錄……………………………………………………………………IV
圖目錄…………………………………………………………………VI
第一章 緒論...............................................................................................1
1-1 前言..............................................................................................1
1-2 研究內容......................................................................................2
第二章 共焦拉曼與螢光顯微鏡之原理與設計......................................4
2-1 共焦顯微鏡的介紹....................................................................4
2-1-1 共焦顯微鏡之原理及優點.............................................4
2-1-2 共焦顯微鏡之理論.........................................................6
2-2 拉曼效應之機制........................................................................9
2-3 共焦拉曼與螢光顯微鏡.........................................................11
2-3-1 光學架構.........................................................................11
2-3-2 光譜校正.........................................................................18
第三章 飛秒級脈衝雷射沉積系統........................................................21
3-1 脈衝雷射沉積法簡介.............................................................21
3-2 脈衝雷射沉積法的機制.........................................................22
3-3 PLD 光路.................................................................................24
3-4 實驗硬體設備.........................................................................25
第四章 用Femtosecond laser PLD 成長出特殊結構的碳薄膜............28
4-1 實驗方法.................................................................................28
4-2 實驗結果-掃描式電子顯微鏡（SEM）掃描.......................30
4-3 實驗結果-原子力顯微鏡（AFM）掃描..............................33
4-4 實驗結果-用共焦拉曼顯微鏡進行拉曼訊號量測...............34
第五章 用共焦螢光顯微鏡診斷鑽石中NV defect 的空間分佈..........39
5-1 Nitrogen-Vacancy defect 的結構及螢光特性.......................39
5-2 實驗結果-螢光譜線量測.......................................................41
5-3 實驗結果- NV-的空間分佈....................................................43
5-4 實驗結果-系統的縱向解析度...............................................46
第六章 結論及後續工作........................................................................47
附錄...........................................................................................................48
參考文獻...................................................................................................55
參考文獻 【1】 P. Davidovits and M. D. Egger, “Scanning laser microscope”, Nature, Vol. 223, pp. 831, 1869.
【2】 G. Q. Xiai, T. R. Corle, and G. S. Kine, “Real-time confocal scanning optical microscope”, Appl. Phy. Lett., Vol. 53, pp. 716, 1988.
【3】 W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy”, Science, Vol. 248, pp. 73-76, 1990.
【4】 李超煌， “差動共焦顯微術及其應用”，國立台灣大學， 博士論文，民國86年
【5】 M. Born, and E. Wolf, Principles of Optics, Pergamon Press, 1980.
【6】 C. J. R. Sheppard and D. M. Shotton, Confocal Laser Scanning Microscopy, Ch3, 1997.
【7】 莫定山，“拉曼光譜原理及應用”
【8】 HAMAMATSU, Photon Counting using Photomutiplier Tube, 2001.
【9】 Michael N. R. Ashfold, Frederik Claeyssens, Gareth M. Fuge and Simon J. Henley, Pulsed laser ablation and deposition of thin films, CSR 33, 23 (2004)
【10】 葉啟宇， “以脈衝雷射沉積製造FeBO3薄膜”，國立中央大學， 碩士論文，民國99年
【11】 K. J. Koivusaari, et. al. “Pulsed-laser deposition of diamond-like carbon- Relations between laser fluence, velocity of carbon ions, and bonding in the films”, J. Appl. Phy., Vol. 85, pp. 2915, 1999.
【12】 F. Qian et. al. “High intensity femtosecond laser deposition of diamond-like carbon thin films”, J. Appl. Phy., Vol. 86, pp. 2281, 1999.
【13】 F. Garrelie et. al. “Femtosecond pulsed laser deposition of diamond-like carbon thin films for tribological applications”, Surface and Coatings Technology, Vol.163 –164, pp. 306–312, 2003.
【14】 A. C. Ferrari and J. Robertson, “Interpretation of Raman spectra of disordered and amorphous carbon”, Phy. Rev. B, Vol. 61, pp. 20, 2000.
【15】 http://en.wikipedia.org/wiki/Nitrogen-vacancy_center
【16】 J. Martin et. al. “Generation and detection of fluorescent color centers in diamond with submicron resolution”, Appl. Phy. Lett., Vol. 75, pp. 20, 1999.
指導教授 陳賜原(Szu-yuan Chen) 審核日期 2010-7-28 推文 plurk
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