俱螢光反應之生物感測晶片研發與在人體血清白蛋白應用

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姓名

廖君宇(Chun-Yu Liao) 查詢紙本館藏

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電機工程學系

論文名稱

俱螢光反應之生物感測晶片研發與在人體血清白蛋白應用
(The Research of Fluorescence Reaction Bio-sensor and its Application in Human Serum Albumin)

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摘要(中)

本研究整合半導體製程技術、以及化學生物標定法，來進行人體血清白蛋白之感測。感測器元件材料為砷化鎵，並使用半導體微影製程製作異質接面光電晶體，再利用生物螢光法做生物分子標記。其原理乃利用標記後化合物躍昇至激發態的螢光為生物訊號，對人體血清白蛋白進行感測。
研究中分別對感測器之靈敏度及再現性作多次的測試分析，於再現性的表現上，在待測溶液位於相同濃度之感測結果，其標準差僅達3.39 × 10-9 A；而靈敏度的範圍，經由多次量測分析，在待測溶液濃度介於0.01 mg/mL ~ 0.07 mg/mL之間，其光電流變化大小呈現線性的結果。實驗中並將感測器與微流道系統結合做一完整的量測平台，對量測數據進行分析後得到為Y = 1.6 × 10-6 + 1.38 × 10-8X之關係式，其中的Y值為光電流的大小，單位是安培，X值為HSA溶液之濃度，單位為每微克/毫升。此關係式代表此感測器在HSA溶液濃度介於0.01 mg/mL ~ 0.07 mg/mL之間，濃度每增加1 μg/mL，其光電流的反應約會增加13.8 nA的大小。

摘要(英)

In this research, we present bio-sensors fabricated by the semiconductor fabrication and biomark techniques for sensing of Human Serum Albumin (HSA). The GaAs was used as the sensing material to fabricate heterojunction phototransistor array as the sensing device, and the fluorescence phenomenon of the biomark compound was designed as the signal. By combining the sensing device and biomark, a biosensor for HSA detection is demonstrated.
Numerous of experiments were tested for the sensitivity and reproducibility of the fabricated sensors. For the reproducibility test, the conditions at the same concentration of HSA were taken for the detection, and the standard deviation of the results was only 3.39 × 10-9 A. And for the sensitivity test, the experiments showed that the sensor demonstrated a linear result in the increase of photo-induced current at the HSA solution concentration of between 0.01 mg/mL and 0.07 mg/mL. Moreover, a micro-flow system of integrating the sensor-array and feed-through setup was established, the experiments showed similar results to the single sensor. The results present a trend line of Y = 1.6 × 10-6 + 1.38 × 10-8X, where Y is the magnitude of photo-induced current, and X is the concentration of HSA solution. It shows that the magnitude of photo-induced current is increased by 13.8 nA when the concentration of HSA solution increases a microgram between 0.01 mg/mL to 0.07 mg/mL.

關鍵字(中)

★ 生物晶片
★ 螢光
★ 光檢測器
★ 人體血清白蛋白
★ 光電晶體

關鍵字(英)

★ fluorescence
★ photonic
★ bio
★ sensor
★ HSA
★ phototransistor

論文目次

摘要 I
Abstract II
圖目錄 V
表目錄 VII
第一章導論 1
1.1 生物感測器之發展與應用 1
1.2 生物感測器之簡介 1
1.3 研究動機 8
1.4 研究摘要 9
第二章原理及研究方法 10
2.1 簡介 10
2.2 光電晶體感測器 10
2.2.1 光電晶體之簡介 10
2.2.2 光電晶體之工作原理 11
2.3 化學生物標定技術 15
2.3.1 生物標記螢光法之簡介 15
2.3.2 標定化合物之特性 17
2.4 結論 18
第三章感測器設計及製作 19
3.1 簡介 19
3.2 光罩設計與感測器結構 19
3.3 感測器製作流程 22
3.4 感測器之特性量測 32
3.5 結論 35
第四章檢測平台的架設、實驗流程與結果討論 37
4.1 簡介 37
4.2 待測溶液的製備 37
4.3 滴定法量測待測溶液 38
4.3.1 實驗流程 38
4.3.2 激發光源的選擇 39
4.3.3 量測條件之設定 44
4.3.4 量測結果與討論 49
4.4 定流法量測待測溶液 51
4.4.1 儀器設備 51
4.4.2 量測環境之設定 54
4.4.3 量測結果與討論 55
4.5 結論 57
第五章總結 59
參考文獻 61

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指導教授

辛裕明(Yue-ming Hsin)

審核日期

2009-7-18

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