博碩士論文 982204016 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:23 、訪客IP:18.119.118.99
姓名 葉如芳(Ju-fang Yeh)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 探討αAmy3、OsCIN1與Os33KD信號肽在水稻懸浮培養細胞中的功能及特性
(Characterized analysis of αAmy3, OsCIN1 and Os33KD signal peptide in rice cell suspension culture)
相關論文
★ 水稻CAF1基因之功能分析-水稻CAF1基因的選殖、定性及表現★ 水稻OsDEADl-1基因的功能性探討
★ 利用水稻細胞之懸浮培養建立蛋白質高效率分泌系統★ 水稻CCR4基因之功能分析- 水稻CCR4基因的選殖、定性及表現
★ 阿拉伯芥 AtMYBS 基因功能性探討★ 水稻OsMYBS2基因的功能性分析
★ 水稻CCR4基因的功能分析- 繁衍大量表現和靜默表現的基因轉殖水稻★ 水稻OsVALs基因的功能性分析- 水稻OsVALs基因的選殖、定性及表現
★ 分析水稻T-DNA插入突變株: M0022150, M0023563, M0023580, M0037352及M0032079★ 以水稻懸浮培養細胞蛋白質生產系統生產mGMCSF
★ 建立表現耐熱澱粉普魯南糖酶基因之轉植甘藷★ 阿拉伯芥AtMYBSs基因參與在糖訊息及離層酸訊息傳遞之研究
★ I. II.★ 水稻CAF1基因在水稻懸浮培養細胞之研究
★ 探討阿拉伯芥兩個MYB-related轉錄因子在糖訊息傳遞中所扮演的角色★ 水稻中五個DEAD-box RNA helicase - RH2、RH6、RH22、RH42和RH51基因之探討
檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   至系統瀏覽論文 ( 永不開放)
摘要(中) 生物工程技術的應用有效地提供了重組蛋白質的生產。由不同類型的寄主细胞所生產的重組蛋白質可能會有不同的生物活性和蛋白質的穩定度,尤其是醫藥蛋白等高價值蛋白質。若要生產具有完整生物活性的高價值重組蛋白質,則以具備完整蛋白質後修飾作用的哺乳類動物細胞和植物寄主细胞作為生產平台之優先考量。在過去幾年,植物分子農場被認作為是一個兼具便利和濟策略表達外源蛋白質生產平台,例如抗體,醫藥蛋白,細胞素等等。水稻細胞生產系統為一有效率的重組蛋白質生產系统。為了要改良重組蛋白質在水稻細胞生產系統中之分泌效率,三種不同的信號肽包括αAmy3,OsCIN1 和Os33KD 融合到GFP 和mGMCSF 基因之N 終端序列,藉由分析轉殖細胞內外的重組蛋白質累積量,以探討三種信號肽之分泌效率。包含有三種不同信號肽的GFP 轉殖細胞株被建立,並且細胞內蛋白質和細胞外蛋白質由西方點墨法或FLUOROSK ANASCENTFL
來偵測重組蛋白質的累積量,而每一種信號肽之分泌效率在各自獨立的穩定轉殖水稻懸浮細胞株中進行比較。實驗結果顯示,Os33KD 信號肽相對其他兩者而言在GFP 水稻細胞懸浮培養有糖中有最高的分泌效率,而αAmy3 和OsCIN1 信號肽在缺醣處理下顯示了在缺醣情況下其分泌效率有被提升的現象。因此我們發現,在有糖誘導表現量較強的Ubiquitin 啟動子,使用αAmy3 和OsCIN1 信號肽會有較佳的分泌比例的表現。藉由對信號肽更多的探討及研究,我們能夠知道不同的生長環境可能會影響信號肽之分泌功能,深入認識信號肽之特性能夠幫助我們在不同的生長條件下選擇不同的信號肽,以期在植物細胞生產平台能夠達到更大量的重組蛋白質之產量。
摘要(英) Application of biotechnical approach has provided to be useful in the production of
recombinant proteins. Recombinant proteins produced from different types of host
cell have various biological activities and protein stability. To produce bioactive
recombinant protein, mammal and plant host cells were used due to they have
post-translational modification. In the past few years, plant molecular farming is
considered as a convenient and economical production strategy to express foreign
proteins, such as antibody, pharmaceutical medicine, cytokine, and so on. The rice
cell production system is a useful recombinant protein production system. To
improve the secretion efficiency of recombinant proteins in rice expression system,
different signal peptides including αAmy3, OsCIN1 and Os33KD were fused to the
N-terminal of GFP and mGMCSF respectively to explore the secretary efficiency of
foreign proteins. Three different signal peptides fused to N-terminal GFP in
transgenic rice cell lines were generated, and the intracellular proteins and
extracellular proteins was detected by western blotting or FLUOROSKAN
ASCENTFL. Therefore, the secretion efficiency of each signal peptide can be
compared among different stable transformed rice suspension cell lines. The result
showed that the Os33KD signal peptide has the highest secretion ratio in GFP
transgenic rice cell suspension culture containing sucrose, but the αAmy3 and OsCIN1 signal peptides showed an increase in secretion during sugar starvation
treatment. The efficiencies of signal peptides that are able to secret more
recombinant proteins into culture medium is different according to the growth
condition, therefore we can choose the suitable signal peptide in rice suspension cell
bioreactor.
關鍵字(中) ★ 信號肽
★ 水稻懸浮培養細胞
關鍵字(英) ★ signal peptide
★ rice cell suspension culture
論文目次 圖目錄 IVV
表目錄 IVV
結果圖目錄 ........................................................................................................................... IVV
第一章 前人研究 ................................................................................................................ 1
一、 基因工程 ............................................................................................................ 1
二、 重組蛋白質在現今各領域之應用 .................................................................... 2
1. 在醫學研究方面: ........................................................................................ 2
2. 在農業方面: ................................................................................................ 3
3. 在工業及環境污染防治方面: .................................................................... 3
三、 重組蛋白質表達系統 ........................................................................................ 4
1. 大腸桿菌表達系統 ........................................................................................ 5
2. 酵母菌表達系統 ............................................................................................ 5
3. 昆蟲表達系統 ................................................................................................ 6
4. 哺乳類動物表達系統 .................................................................................... 7
5. 植物表達系統 ................................................................................................ 8
四、 詳述植物表達系統在現今各領域中之發展 .................................................... 9
五、 植物表達系統的改良策略 .............................................................................. 11
1. 啓動子的改良 (promoter) ......................................................................... 11
2. 內插子 (intron) ........................................................................................... 12
3. 信號肽 (signal peptide) .............................................................................. 12
4. 水稻懸浮培養系統 (rice cell suspension culture system) ....................... 13
第二章 研究目的及相關背景說明 .................................................................................. 15
一、 研究動機與目的 .............................................................................................. 15
二、 研究策略 .......................................................................................................... 15
1. 表達載體的構築 .......................................................................................... 15
2. 建立轉殖水稻細胞株 .................................................................................. 20
3. 重組蛋白質之定量定性分析 ...................................................................... 21
第三章 MATERIALS AND METHODS .......................................................................................... 22
1. Establishment of GFP and mGMCSF transgenic rice cell lines ................. 22
1.1 Rice callus induction ................................................................................... 22
1.2 Transformation competent Agrobacterium strain EHA105 .................... 22
1.3 Transformation by particle bombardment .............................................. 22
1.4 Extraction of rice genomic DNA and RNA .............................................. 23
1.5 Rice genomic PCR and RT-PCR ............................................................... 24
1.6 GUS staining ............................................................................................... 24
III
2. Analysis of expression recombinant protein ................................................ 25
2.1 Establish transgenic rice suspension culture system ............................... 25
2.2 Western blotting .......................................................................................... 25
2.3 GFP-specific detection by FLUOROSKAN ASCENTFL ....................... 26
第四章 結果 ............................................................................................................................ 27
一、 高效率信號肽融合GFP 表現量細胞之篩選與確認 .................................... 27
1. 建立信號肽融合之GFP 轉殖細胞 ............................................................ 27
2. 轉殖水稻細胞株信號肽融合GFP 蛋白質之分泌效率分析 .................... 28
3. 轉殖水稻細胞株信號肽融合GFP 蛋白質在有醣缺醣情況下分泌效率之
分析 30
二、 高效率信號肽融合mGMCSF 表現量細胞之篩選與確認 .......................... 32
1 建立信號肽融合之mGMCSF 轉殖細胞 .................................................. 32
2 轉殖水稻細胞株信號肽融合mGMCSF 蛋白質之分泌效率分析 .......... 33
第五章 討論 ............................................................................................................................ 35
第六章參考文獻 .................................................................................................................... 54
附錄一、實驗所使用之引子序列 .......................................................................................... 63
附錄二、實驗信號肽序列及其胺基酸組成 .......................................................................... 64
附錄三、研究材料配置 .......................................................................................................... 65
I Culture Medium ........................................................................................................ 65
II Analysis reagent ....................................................................................................... 69
參考文獻 吳文騰。生技醫藥,生物產業技術概論,清華大學出版社,2003。
王升陽,徐麗芬,楊寧蓀。植物基因轉殖在醫藥上之應用,植物基因轉殖之原理與
應用,教育部出版,2004。
劉馨如。利用水稻細胞之懸浮培養建立蛋白質高效率分泌系統,碩士論文,2006。
賴穎慧。以水稻懸浮培養細胞蛋白質生產系統生產mGMCSF,碩士論文,2009。
林長平,劉瑞芬,莊榮輝,丁詩同,李宣書等編著。生物技術概論,華杏出版,2009。
(1999) Yeast Industry Platform. Yeast 15:911-912.
(2007) Another inconvenient truth. In Europe, no one apparently wants to listen if you
have good news about genetically modified organisms (GMOs). Nat Biotechnol
25:1330.
Adeniran AJ, Shoshani I, Minuth M, Awad JA, Elce JS, Johnson RA (1995) Purification,
characterization, and N-terminal amino acid sequence of the adenylyl
cyclase-activating protease from bovine sperm. Biol Reprod 52:490-499.
Agrawal V, Gupta B, Banerjee UC, Roy N (2008) A method for construction, cloning and
expression of intron-less gene from unannotated genomic DNA. Mol Biotechnol
40:217-223.
Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G (2003)
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves
plant vigor under favorable growth conditions but not under drought or salt stress.
Plant Cell 15:439-447.
Alvarez ML, Pinyerd HL, Crisantes JD, Rigano MM, Pinkhasov J, Walmsley AM,
Mason HS, Cardineau GA (2006) Plant-made subunit vaccine against pneumonic
and bubonic plague is orally immunogenic in mice. Vaccine 24:2477-2490.
Arredondo-Peter R, Moran JF, Sarath G, Luan P, Klucas RV (1997) Molecular cloning of
the cowpea leghemoglobin II gene and expression of its cDNA in Escherichia
coli. Purification and characterization of the recombinant protein. Plant Physiol
114:493-500.
Au LC, Liu TJ, Shen HD, Choo KB, Wang SY (1996) Secretory production of bioactive
recombinant human granulocyte-macrophage colony-stimulating factor by a
baculovirus expression system. J Biotechnol 51:107-113.
Awram P, Gardner RC, Forster RL, Bellamy AR (2002) The potential of plant viral
vectors and transgenic plants for subunit vaccine production. Adv Virus Res
58:81-124.
Azbel MY (1980) DNA sequencing and helix-coil transition. III. DNA sequencing.
55
Biopolymers 19:95-109.
Baneyx F, Mujacic M (2004) Recombinant protein folding and misfolding in Escherichia
coli. Nat Biotechnol 22:1399-1408.
Barnes LM, Bentley CM, Dickson AJ (2000) Advances in animal cell recombinant
protein production: GS-NS0 expression system. Cytotechnology 32:109-123.
Barzegari A, Hejazi MA, Hosseinzadeh N, Eslami S, Mehdizadeh Aghdam E, Hejazi MS
(2010) Dunaliella as an attractive candidate for molecular farming. Mol Biol Rep
37:3427-3430.
Basaran P, Rodriguez-Cerezo E (2008) Plant molecular farming: opportunities and
challenges. Crit Rev Biotechnol 28:153-172.
Belbahri L, Chevalier L, Bensaddek L, Gillet F, Fliniaux MA, Boerjan W, Inze D,
Thomas D, Thomasset B (2000) Different expression of an S-adenosylmethionine
synthetase gene in transgenic tobacco callus modifies alkaloid biosynthesis.
Biotechnol Bioeng 69:11-20.
Bennett RP, Cox CA, Hoeffler JP (1998) Fusion of green fluorescent protein with the
Zeocin-resistance marker allows visual screening and drug selection of
transfected eukaryotic cells. Biotechniques 24:478-482.
Bermudez LE, Martinelli J, Petrofsky M, Kolonoski P, Young LS (1994) Recombinant
granulocyte-macrophage colony-stimulating factor enhances the effects of
antibiotics against Mycobacterium avium complex infection in the beige mouse
model. J Infect Dis 169:575-580.
Bernard AR, Kost TA, Overton L, Cavegn C, Young J, Bertrand M, Yahia-Cherif Z,
Chabert C, Mills A (1994) Recombinant protein expression in a Drosophila cell
line: comparison with the baculovirus system. Cytotechnology 15:139-144.
Birch JR, Froud SJ (1994) Mammalian cell culture systems for recombinant protein
production. Biologicals 22:127-133.
Blande JD, Holopainen JK, Li T (2010) Air pollution impedes plant-to-plant
communication by volatiles. Ecol Lett 13:1172-1181.
Blande JD, Li T, Holopainen JK (2011) Air pollution impedes plant-to-plant
communication, but what is the signal? Plant Signal Behav 6.
Bock R (2007) Plastid biotechnology: prospects for herbicide and insect resistance,
metabolic engineering and molecular farming. Curr Opin Biotechnol 18:100-106.
Boothe J, Nykiforuk C, Shen Y, Zaplachinski S, Szarka S, Kuhlman P, Murray E, Morck
D, Moloney MM (2010) Seed-based expression systems for plant molecular
farming. Plant Biotechnol J 8:588-606.
Boulter D, Edwards GA, Gatehouse AMR, Gatehouse JA, Hilder VA (1990) Additive
Protective Effects of Different Plant-Derived Insect Resistance Genes in
Transgenic Tobacco Plants. Crop Prot 9:351-354.
56
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram
quantities of protein utilizing the principle of protein-dye binding. Anal Biochem
72:248-254.
Brandalise M, Maia IG, Borecky J, Vercesi AE, Arruda P (2003) Overexpression of plant
uncoupling mitochondrial protein in transgenic tobacco increases tolerance to
oxidative stress. J Bioenerg Biomembr 35:203-209.
Chakraborty C (2004) Overexpression for commercial production of recombinant human
insulin as A-chain and B-chain fusion protein in Escherichia coli through
genetically engineered plasmids. Indian J Pathol Microbiol 47:569-573.
Chang CN, Matteucci M, Perry LJ, Wulf JJ, Chen CY, Hitzeman RA (1986)
Saccharomyces cerevisiae secretes and correctly processes human interferon
hybrid proteins containing yeast invertase signal peptides. Mol Cell Biol
6:1812-1819.
Chaubet-Gigot N, Kapros T, Flenet M, Kahn K, Gigot C, Waterborg JH (2001)
Tissue-dependent enhancement of transgene expression by introns of replacement
histone H3 genes of Arabidopsis. Plant Mol Biol 45:17-30.
Chen Q, Wang C, Chen D, Chen J, Jiang C, Zheng H (2006) Construction and expression
of human PTEN tumor suppressor gene recombinant adenovirus vector. J
Huazhong Univ Sci Technolog Med Sci 26:325-328.
Chen TL, Lin YL, Lee YL, Yang NS, Chan MT (2004) Expression of bioactive human
interferon-gamma in transgenic rice cell suspension cultures. Transgenic Res
13:499-510.
Cheon SH, Lee KH, Kwon JY, Choi SH, Song MN, Kim DI (2009) Enhanced delivery of
siRNA complexes by sonoporation in transgenic rice cell suspension cultures. J
Microbiol Biotechnol 19:781-786.
Chia MY, Hsiao SH, Chan HT, Do YY, Huang PL, Chang HW, Tsai YC, Lin CM, Pang
VF, Jeng CR (2011) Evaluation of the immunogenicity of a transgenic tobacco
plant expressing the recombinant fusion protein of GP5 of porcine reproductive
and respiratory syndrome virus and B subunit of Escherichia coli heat-labile
enterotoxin in pigs. Vet Immunol Immunopathol 140:215-225.
Choi T, Huang M, Gorman C, Jaenisch R (1991) A generic intron increases gene
expression in transgenic mice. Mol Cell Biol 11:3070-3074.
Cholak J, Schafer LJ, Yeager D (1954) Air pollution near and iron slag processing plant.
Am Ind Hyg Assoc Q 15:220-225.
Conesa C, Calvo M, Sanchez L (2010) Recombinant human lactoferrin: a valuable
protein for pharmaceutical products and functional foods. Biotechnol Adv
28:831-838.
Conner AJ, Christey MC (1994) Plant-Breeding and Seed Marketing Options for the
57
Introduction of Transgenic Insect-Resistant Crops. Biocontrol Sci Techn
4:463-473.
Cozzone AJ (2002) Genetically modified organisms: novel technologies and applications.
Biochimie 84:1045.
Cregg JM, Cereghino JL, Shi J, Higgins DR (2000) Recombinant protein expression in
Pichia pastoris. Mol Biotechnol 16:23-52.
Damert A, Leibiger B, Leibiger IB (1996) Dual function of the intron of the rat insulin I
gene in regulation of gene expression. Diabetologia 39:1165-1172.
Daniell H, Streatfield SJ, Wycoff K (2001) Medical molecular farming: production of
antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci
6:219-226.
Drake PM, Thangaraj H (2010) Molecular farming, patents and access to medicines.
Expert Rev Vaccines 9:811-819.
Edwards MC, Henriksen ED, Yomano LP, Gardner BC, Sharma LN, Ingram LO, Doran
Peterson J (2011) Addition of Genes for Cellobiase and Pectinolytic Activity in
Escherichia coli for Fuel Ethanol Production from Pectin-Rich Lignocellulosic
Biomass. Appl Environ Microbiol 77:5184-5191.
Emanuelsson O, Nielsen H, Brunak S, von Heijne G (2000) Predicting subcellular
localization of proteins based on their N-terminal amino acid sequence. J Mol
Biol 300:1005-1016.
Epstein J, Lee MM, Kelly CE, Donahoe PK (1990) Effect of E. coli endotoxin on
mammalian cell growth and recombinant protein production. In Vitro Cell Dev
Biol 26:1121-1122.
Fischer R, Drossard J, Emans N, Commandeur U, Hellwig S (1999) Towards molecular
farming in the future: pichia pastoris-based production of single-chain antibody
fragments. Biotechnol Appl Biochem 30 ( Pt 2):117-120.
Fischer R, Emans N (2000) Molecular farming of pharmaceutical proteins. Transgenic
Res 9:279-299; discussion 277.
Fischer R, Hoffmann K, Schillberg S, Emans N (2000) Antibody production by
molecular farming in plants. J Biol Regul Homeost Agents 14:83-92.
Fu LB, Yu JL, Liu WH (2011) [Biological characteristics of defensin and its
disease-resistance genetic engineering]. Yi Chuan 33:512-519.
Fujiki Y, Ito M, Nishida I, Watanabe A (2000) Multiple signaling pathways in gene
expression during sugar starvation. Pharmacological analysis of din gene
expression in suspension-cultured cells of Arabidopsis. Plant Physiol
124:1139-1148.
Futatsumori-Sugai M, Tsumoto K (2010) Signal peptide design for improving
recombinant protein secretion in the baculovirus expression vector system.
58
Biochem Biophys Res Commun 391:931-935.
Ghonaim SA, Abou-Zeid AA, Abd El-Fattah AF, Farid MA (1980) Utilization of waste
products of dehydrated onion industry for production of fodder yeast by
Saccharomyces cerevisiae. Zentralbl Bakteriol Naturwiss 135:82-95.
Gill RT, Cha HJ, Jain A, Rao G, Bentley WE (1998) Generating controlled reducing
environments in aerobic recombinant Escherichia coli fermentations: effects on
cell growth, oxygen uptake, heat shock protein expression, and in vivo CAT
activity. Biotechnol Bioeng 59:248-259.
Goud GN, Zhan B, Ghosh K, Loukas A, Hawdon J, Dobardzic A, Deumic V, Liu S,
Dobardzic R, Zook BC, Jin Q, Liu Y, Hoffman L, Chung-Debose S, Patel R,
Mendez S, Hotez PJ (2004) Cloning, yeast expression, isolation, and vaccine
testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from
Ancylostoma ceylanicum. J Infect Dis 189:919-929.
Hamauzu Z, Hiromi K, Ono S (1965) Quantitative Determination of Anomeric Forms of
Sugar Produced by Amylases. 3. Taka-Amylase a--Phenyl-Alpha-Maltoside
System. J Biochem 57:42-44.
Hiatt A, Cafferkey R, Bowdish K (1989) Production of antibodies in transgenic plants.
Nature 342:76-78.
Hirai H, Kashima Y, Hayashi K, Sugiura T, Yamagishi K, Kawagishi H, Nishida T (2008)
Efficient expression of laccase gene from white-rot fungus Schizophyllum
commune in a transgenic tobacco plant. FEMS Microbiol Lett 286:130-135.
Hitzeman RA, Chang CN, Chen CY, Singh A, Etcheverry T, Chisholm V, Kohr W, Vehar
G, Renz M, Dowbenko D (1988) Protein products from yeast. Biochem Soc Trans
16:1081.
Hitzeman RA, Chen CY, Dowbenko DJ, Renz ME, Liu C, Pai R, Simpson NJ, Kohr WJ,
Singh A, Chisholm V, et al. (1990) Use of heterologous and homologous signal
sequences for secretion of heterologous proteins from yeast. Methods Enzymol
185:421-440.
Hitzeman RA, Hagie FE, Levine HL, Goeddel DV, Ammerer G, Hall BD (1981)
Expression of a human gene for interferon in yeast. Nature 293:717-722.
Hitzeman RA, Leung DW, Perry LJ, Kohr WJ, Levine HL, Goeddel DV (1983)
Secretion of human interferons by yeast. Science 219:620-625.
Holst-Jensen A, Ronning SB, Lovseth A, Berdal KG (2003) PCR technology for
screening and quantification of genetically modified organisms (GMOs). Anal
Bioanal Chem 375:985-993.
Hong SY, Kwon TH, Jang YS, Kim SH, Yang MS (2006) Production of bioactive human
granulocyte-colony stimulating factor in transgenic rice cell suspension cultures.
Protein Expr Purif 47:68-73.
59
Hong SY, Lee TS, Kim J, Jung JH, Choi CW, Kim TG, Kwon TH, Jang YS, Yang MS
(2008) Tumor targeting of humanized fragment antibody secreted from transgenic
rice cell suspension culture. Plant Mol Biol 68:413-422.
Hood EE, Kusnadi A, Nikolov Z, Howard JA (1999) Molecular farming of industrial
proteins from transgenic maize. Adv Exp Med Biol 464:127-147.
Hu YC (2005) Baculovirus as a highly efficient expression vector in insect and
mammalian cells. Acta Pharmacol Sin 26:405-416.
Huang LF, Liu YK, Lu CA, Hsieh SL, Yu SM (2005) Production of human serum
albumin by sugar starvation induced promoter and rice cell culture. Transgenic
Res 14:569-581.
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a
sensitive and versatile gene fusion marker in higher plants. EMBO J
6:3901-3907.
Kim NS, Yu HY, Chung ND, Shin YJ, Kwon TH, Yang MS (2011) Production of
functional recombinant bovine trypsin in transgenic rice cell suspension cultures.
Protein Expr Purif 76:121-126.
Kim TG, Kim HM, Lee HJ, Shin YJ, Kwon TH, Lee NJ, Jang YS, Yang MS (2007)
Reduced protease activity in transformed rice cell suspension cultures expressing
a proteinase inhibitor. Protein Expr Purif 53:270-274.
Kim TG, Lee HJ, Jang YS, Shin YJ, Kwon TH, Yang MS (2008) Co-expression of
proteinase inhibitor enhances recombinant human granulocyte-macrophage
colony stimulating factor production in transgenic rice cell suspension culture.
Protein Expr Purif 61:117-121.
Kwon TH, Seo JE, Kim J, Lee JH, Jang YS, Yang MS (2003) Expression and secretion
of the heterodimeric protein interleukin-12 in plant cell suspension culture.
Biotechnol Bioeng 81:870-875.
Le Roy K, Lammens W, Verhaest M, De Coninck B, Rabijns A, Van Laere A, Van den
Ende W (2007) Unraveling the difference between invertases and fructan
exohydrolases: a single amino acid (Asp-239) substitution transforms
Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase. Plant Physiol
145:616-625.
Liu X, Broberg E, Watanabe D, Dudek T, Deluca N, Knipe DM (2009) Genetic
engineering of a modified herpes simplex virus 1 vaccine vector. Vaccine
27:2760-2767.
Lu G, Zou Q, Guo D, Zhuang X, Yu X, Xiang X, Cao J (2007) Agrobacterium
tumefaciens-mediated transformation of Narcissus tazzeta var. chinensis. Plant
Cell Rep 26:1585-1593.
Ma JK, Barros E, Bock R, Christou P, Dale PJ, Dix PJ, Fischer R, Irwin J, Ma
Pezzotti M, Schillberg S, Sparrow P, Stoger E, Twyman RM (2005) Molecular
farming for new drugs and vaccines. Current perspectives on the production of
pharmaceuticals in transgenic plants. EMBO Rep 6:593-599.
Maeda S, Kawai T, Obinata M, Fujiwara H, Horiuchi T, Saeki Y, Sato Y, Furusawa M
(1985) Production of human alpha-interferon in silkworm using a baculovirus
vector. Nature 315:592-594.
Matsumoto Y, Suzuki S, Nozoye T, Yamakawa T, Takashima Y, Arakawa T, Tsuji N,
Takaiwa F, Hayashi Y (2009) Oral immunogenicity and protective efficacy in
mice of transgenic rice plants producing a vaccine candidate antigen (As16) of
Ascaris suum fused with cholera toxin B subunit. Transgenic Res 18:185-192.
McCall EJ, Danielsson A, Hardern IM, Dartsch C, Hicks R, Wahlberg JM, Abbott WM
(2005) Improvements to the throughput of recombinant protein expression in the
baculovirus/insect cell system. Protein Expr Purif 42:29-36.
Miao CH, Ohashi K, Patijn GA, Meuse L, Ye X, Thompson AR, Kay MA (2000)
Inclusion of the hepatic locus control region, an intron, and untranslated region
increases and stabilizes hepatic factor IX gene expression in vivo but not in vitro.
Mol Ther 1:522-532.
Nishimura A, Aichi I, Matsuoka M (2006) A protocol for Agrobacterium-mediated
transformation in rice. Nat Protoc 1:2796-2802.
Obembe OO, Popoola JO, Leelavathi S, Reddy SV (2011) Advances in plant molecular
farming. Biotechnol Adv 29:210-222.
Panahi M, Alli Z, Cheng X, Belbaraka L, Belgoudi J, Sardana R, Phipps J, Altosaar I
(2004) Recombinant protein expression plasmids optimized for industrial E. coli
fermentation and plant systems produce biologically active human insulin-like
growth factor-1 in transgenic rice and tobacco plants. Transgenic Res
13:245-259.
Sailaja M, Tarakeswari M, Sujatha M (2008) Stable genetic transformation of castor
(Ricinus communis L.) via particle gun-mediated gene transfer using embryo
axes from mature seeds. Plant Cell Rep 27:1509-1519.
Shin YJ, Hong SY, Kwon TH, Jang YS, Yang MS (2003) High level of expression of
recombinant human granulocyte-macrophage colony stimulating factor in
transgenic rice cell suspension culture. Biotechnol Bioeng 82:778-783.
Sijmons PC, Dekker BM, Schrammeijer B, Verwoerd TC, van den Elzen PJ, Hoekema A
(1990) Production of correctly processed human serum albumin in transgenic
plants. Biotechnology (N Y) 8:217-221.
Simmons CW, VanderGheynst JS (2007) Transient co-expression of post-transcriptional
gene silencing suppressors and beta-glucuronidase in harvested lettuce leaf tissue
does not improve recombinant protein accumulation in planta. Biotechnol Lett
61
29:641-645.
Skory CD, Freer SN, Bothast RJ (1996) Expression and secretion of the Candida
wickerhamii extracellular beta-glucosidase gene, bglB, in Saccharomyces
cerevisiae. Curr Genet 30:417-422.
Stewart LM, Hirst M, Lopez Ferber M, Merryweather AT, Cayley PJ, Possee RD (1991)
Construction of an improved baculovirus insecticide containing an insect-specific
toxin gene. Nature 352:85-88.
Takaiwa F (2009) Allergen-specific immunotherapy with plant-based oral vaccines.
Immunotherapy 1:517-519.
Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R (2003) Molecular farming in
plants: host systems and expression technology. Trends Biotechnol 21:570-578.
van Beek N, Davis DC (2007) Baculovirus insecticide production in insect larvae.
Methods Mol Biol 388:367-378.
Wadensten H, Ekebacke A, Hammarberg B, Holmgren E, Kalderen C, Tally M, Moks T,
Uhlen M, Josephson S, Hartmanis M (1991) Purification and characterization of
recombinant human insulin-like growth factor II (IGF-II) expressed as a secreted
fusion protein in Escherichia coli. Biotechnol Appl Biochem 13:412-421.
Wang L, Shimizu Y, Mizunaga T, Matsumoto S, Otsuka Y (2008) Expression,
purification and characterization of yeast protein disulfide isomerase produced by
a recombinant baculovirus-mediated silkworm, Bombyx mori, pupae expression
system. Biotechnol Lett 30:625-630.
Wickham TJ, Nemerow GR (1993) Optimization of growth methods and recombinant
protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression
system. Biotechnol Prog 9:25-30.
Wojtatowicz M, Kuzniarz M, Sobieszczanski J, Rutkowski M (1981) Synthetic
hydrocarbons obtained by means of coal processing as a raw material for the
microbiological industry. III. Assimilation of individual hydrocarbons from
kogasin by selected yeast strains of the genus Candida. Acta Microbiol Pol
30:165-171.
Xu J, Kieliszewski M (2011) Enhanced accumulation of secreted human growth
hormone by transgenic tobacco cells correlates with the introduction of an
N-glycosylation site. J Biotechnol 154:54-59.
Yan J, Wang Y, Shao SH, Mao YF, Li HW, Luo YH (2004) Construction of prokaryotic
expression system of ltB-ureB fusion gene and identification of the recombinant
protein immunity and adjuvanticity. World J Gastroenterol 10:2675-2679.
Yang L, An X, Wei F, Liu H, Li H, Yu J, Ren X (2008) Expression and purification of
recombinant human interleukin-18 protein using a yeast expression system.
Protein Expr Purif 62:44-48.
62
Yeh CH, Yeh KW, Wu SH, Chang PF, Chen YM, Lin CY (1995) A recombinant rice
16.9-kDa heat shock protein can provide thermoprotection in vitro. Plant Cell
Physiol 36:1341-1348.
Yu TT, Skinner DZ, Liang GH, Trick HN, Huang B, Muthukrishnan S (2000)
Agrobacterium-mediated transformation of creeping bentgrass using GFP as a
reporter gene. Hereditas 133:229-233.
指導教授 陸重安(Chung-an Lu) 審核日期 2011-9-14
推文 facebook   plurk   twitter   funp   google   live   udn   HD   myshare   reddit   netvibes   friend   youpush   delicious   baidu   
網路書籤 Google bookmarks   del.icio.us   hemidemi   myshare   

若有論文相關問題,請聯絡國立中央大學圖書館推廣服務組 TEL:(03)422-7151轉57407,或E-mail聯絡  - 隱私權政策聲明