博碩士論文 985201014 詳細資訊


姓名 魏永年(Yung-nian Wei)  查詢紙本館藏   畢業系所 電機工程學系
論文名稱 利用網印碳電極以交流阻抗法檢測糖化血紅素
(Glycated Hemoglobin Detection with Screen-printed Carbon Electrode by AC Impedance Analysis)
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摘要(中) 本研究利用拋棄式碳電極作為量測工具、交流阻抗法作為檢測分析方法,對血紅素(Hb)與糖化血紅素(HbA1c)進行檢測。拋棄式碳電極以網印方式製成,製作出的電極選用具有較高的相對阻抗差進行實驗量測。由於檢測目標物血紅素與糖化血紅素電特性的不同,在溶液中會產生不同數量的帶電粒子。利用交流阻抗法量測將會由等效電路中元件的改變得到穩定顯著的阻抗差異。藉由此原理可以得知Hb、 HbA1c最大檢測靈敏度範圍與靈敏度。樣本溶血物(hemolystates)在HbA1c不同含量下時,同樣利用交流阻抗法也可得到HbA1c含量不同所相應的阻抗差關係。並藉由交流阻抗法量測原理建立自製阻抗量測平台電路,利用微控制器負責資料傳輸與控制,運用MATLAB進行資料收集與處理。自製電路針對HbA1c量測結果與現行阻抗儀有相近的趨勢,擁有可以彌補現行儀器不易攜帶、昂貴…等問題,搭配拋棄式碳電極可以達成簡易、快速、成本低、攜帶方便的糖化血紅素檢測系統。
摘要(英) This research focused on hemoglobin (Hb) and glycated hemoglobin (HbA1c) detection using disposable carbon electrodes and AC impedance analysis. The disposable carbon electrodes were fabricated by screen-printing technology. Among the three fabricated electrode configurations, the one that led to the largest relative impedance difference was used in the measurement. The natures of Hb and HbA1c are different. They have diffreent amount of charged particles in the solution. By AC impedance analysis, we can obtain stable and significant change in the solution resistance in the equivalent circuit model. By this way we knew the concentration range of Hb and HbA1c corresponding to the maximum detection sensitivity. A self-made circuit was used in the impedance measutement. A microcontroller was responsible for measurement control and data transmission. Measurement data were saved and processed with MATLAB. The HbA1c measurement results were similar with the self-made citcuit and with the commercail impedance analyser, which is expensive and not portable. The self-made citcuit and disposable carbon electrodes have made the measurement easy, fast, inexpensive, and portable.
關鍵字(中) ★ 交流阻抗
★ 網印電極
★ 血紅素
★ 糖化血紅素
關鍵字(英) ★ screen-printing
★ electrode
★ hemoglobin
★ glycated hemoglobin
★ AC impedance analysis
論文目次 摘要 I
ABSTRACT II
致謝 III
目錄 IV
圖目錄 VII
表目錄 XVI
第一章 前言 1
1-1 糖尿病 1
1-1-1 糖尿病簡介 1
1-1-2糖尿病的檢測方法 6
1-2文獻回顧 9
1-2-1生物感測器簡介 9
1-2-2網版印刷生物感測器 12
1-2-3電化學感測器量測方法 22
1-2-4網印技術於生物感測應用 28
第二章 研究動機與目的 32
2-1研究動機 32
2-2研究目標 33
第三章 實驗方法 34
3-1 網版印刷電極 34
3-1-1 商業電極 34
3-1-2自製網版印刷電極 36
3-2 電極分析方法 50
3-2-1儀器設備 50
3-2-2電極電化學特性阻抗量測 54
3-2-3不同溶液阻抗量測 55
3-3 蛋白質實驗步驟方法 56
3-3-1 血紅素阻抗量測 56
3-3-2不同濃度血紅素阻抗量測 57
3-3-2不同濃度糖化血紅素阻抗量測 57
3-3-4糖化血紅素不同百分比含量阻抗量測 58
3-4 蛋白質阻抗實驗方法 59
3-4-1阻抗量測 59
3-4-2 自製量測系統 60
第四章 結果與討論 75
4-1網版印刷電極製程結果 75
4-1-1商業電極 75
4-1-2自製電極 82
4-1-3電極比較 89
4-2網版印刷電極量測結果 92
4-2-1商業電極特性 95
4-2-2自製網版印刷電極特性 98
4-2-3三種電極比較 101
4-3蛋白質阻抗量測結果 106
4-3-1血紅素量測 106
4-3-2不同濃度血紅素量測 116
4-3-3不同濃度糖化血紅素量測 119
4-3-4糖化血紅素不同百分比含量阻抗量測 122
4-4自製量測系統 125
4-4-1電路實現 125
4-4-2量測結果 127
第五章 結論 129
未來展望 132
參考文獻 133
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指導教授 蔡章仁(Jang-Zern Tsai) 審核日期 2011-8-24
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