博碩士論文 992202016 詳細資訊




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姓名 陳彥均(Yan-Jiun Chen)  查詢紙本館藏   畢業系所 物理學系
論文名稱 β-谷固醇對POPE膜物理特性的影響
相關論文
★ 用氘核磁共振儀研究含高濃度麥角脂醇的DPPC人造膜之分子交交互作用★ Fluorescence study of lipid membranes containing sterol
★ 含固醇的脂質雙層膜的形態及相行為的研究★ The effects of composition and thermal history on the properties of supported lipid bilayers
★ The effect of sterol on the POPE/DPPC membranes★ 麥角固醇對含膽固醇的脂雙層膜的影響
★ Deuterium NMR Study of the Effect of Stigmasterol on POPE Membranes★ 運用氘核磁共振儀研究POPC/cholesterol膜之物理性質
★ 模型細胞膜(含有相同碳鏈的PC/PE)存在或缺乏固醇類的物理性質★ 運用氘核磁共振研究DPPC/POPE/sterol人造細胞膜之物理性質
★ Phase Behavior and Molecular Interactions of Membranes Containing Phosphatidylcholines and Sterol: A Deuterium NMR Study★ The physical properties of phytosterol-containing lipid bilayers
★ An AFM Study on Supported Lipid Bilayers with and without Sterol★ 固醇結構對PC膜物理特性的影響
★ 人造細胞膜的相行為及脂質-固醇交互作用之研究★ 非接觸式無機液晶配向與混合型液晶元件之 光電性質研究
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摘要(中) 膽固醇及其他種類的固醇對於形成脂質筏扮演重要的角色,現在普遍認為脂質筏區塊處於lo態。從過去對人造細胞膜的研究可知,固醇可以誘發脂雙層膜形成lo態,但大部分的研究都以PC類脂膜為主,較少對PE類脂膜進行研究。為進一步瞭解脂質筏的形成,探究固醇與PE類脂膜的交互作用是必要的。我們使用氘核磁共振(2H NMR)研究植物固醇對POPE人造細胞膜的物理特性之影響。其方法是先將POPE上sn-1的氫代換成氘,再將其混合不同濃度的植物固醇而成多個樣本,並將各樣本隨溫度改變量測得一系列光譜。 M1(T)值顯示,植物固醇對於gel態及lc態POPE膜的有序程度之影響是不一樣的:植物固醇對gel態膜的有序程度沒有明顯的改變;然而,它會增加lc態POPE膜的有序度,但此影響在植物固醇為20 mol%時達到飽和。此外,分析NMR光譜可得到溫度-濃度相圖,可觀察到兩個兩項共存區。
摘要(英) Cholesterol and other sterols are important for the formation of lipid raft. It is now generally agreed that raft domains are in the liquid-ordered (lo) phase. Previous research on model membranes has found that sterol induces the formation of the lo-phase in lipid bilayers. While most studies were conducted in phosphatidylcholine lipid bilayers, fewer were conducted in phosphoethanolamine (PE) lipid biayers. To further understand the raft formation, exploring the interaction of sterol and PE lipid is necessary.We investigate the effect of phytosterol on the physical properties of 1-[2H31]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance(2H NMR). The sn-1 chain of POPE was deuterium labeled. 2H NMR spectra were taken as a function of temperature andphytosterol concentration. The spectral first moments show that the ordering effects of phytosterol on the gel-phase and liquid crystalline (lc)-phase POPE membranes are different: It has no significant effect on the membrane order in the gel phase.In contrast, it increases the chain order of lc-phase POPE,but this effect saturates at 20 mol% phytosterol.Moreover, the temperature-composition phase diagram, established from the NMR spectra, is presented. Two two-phase coexistence regions were observed.
關鍵字(中) ★ 細胞膜
★ 脂質筏
★ 膜相圖
關鍵字(英) ★ cell membrane
★ lipid rafts
★ phase diagram
論文目次 目錄

提要 I
英文提要 II
致謝 III
目錄 IV
圖目 VI
表目 IX
第一章緒論 1
1.1 生物膜概述 1
1.2 細胞膜結構 1
1.3 脂質分子 3
1.4 膜的相行為 5
1.5POPE與β-sitosterol 7
第二章實驗方法及材料 12
2.12H NMR原理 12
2.1.1共振原理 12
2.1.2 電四極交互作用 14
2.1.3 粉狀光譜(Powder Spectrum) 15
2.1.4 特徵光譜 17
2.1.5 First Moment 的定義 18
2.2 NMR硬體介紹及參數設定 20
2.3 樣本配置 20
第三章結果分析與討論 22
3.1POPE/β-sitosterol生物膜 22
3.2 POPE-d31/β-sitosterol膜的溫度-濃度相圖 36
第四章結論 38
參考文獻 40
參考文獻 參考文獻

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指導教授 薛雅薇(Ya-Wei Hsueh) 審核日期 2014-7-29
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