β-谷固醇對POPE膜物理特性的影響

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：7

、訪客IP：18.118.120.109

姓名

陳彥均(Yan-Jiun Chen) 查詢紙本館藏

畢業系所

物理學系

論文名稱

β-谷固醇對POPE膜物理特性的影響

相關論文

★ 用氘核磁共振儀研究含高濃度麥角脂醇的DPPC人造膜之分子交交互作用	★ Fluorescence study of lipid membranes containing sterol
★ 含固醇的脂質雙層膜的形態及相行為的研究	★ The effects of composition and thermal history on the properties of supported lipid bilayers
★ The effect of sterol on the POPE/DPPC membranes	★ 麥角固醇對含膽固醇的脂雙層膜的影響
★ Deuterium NMR Study of the Effect of Stigmasterol on POPE Membranes	★ Deuterium NMR Study of the effect of 7- dehydrocholesterol on the POPE Membranes
★ 運用氘核磁共振儀研究POPC/cholesterol膜之物理性質	★ 模型細胞膜(含有相同碳鏈的PC/PE)存在或缺乏固醇類的物理性質
★ 運用氘核磁共振研究DPPC/POPE/sterol人造細胞膜之物理性質	★ Phase Behavior and Molecular Interactions of Membranes Containing Phosphatidylcholines and Sterol: A Deuterium NMR Study
★ The physical properties of phytosterol-containing lipid bilayers	★ An AFM Study on Supported Lipid Bilayers with and without Sterol
★ 固醇結構對PC膜物理特性的影響	★ 人造細胞膜的相行為及脂質-固醇交互作用之研究

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

至系統瀏覽論文 ( 永不開放)

摘要(中)

膽固醇及其他種類的固醇對於形成脂質筏扮演重要的角色，現在普遍認為脂質筏區塊處於lo態。從過去對人造細胞膜的研究可知，固醇可以誘發脂雙層膜形成lo態，但大部分的研究都以PC類脂膜為主，較少對PE類脂膜進行研究。為進一步瞭解脂質筏的形成，探究固醇與PE類脂膜的交互作用是必要的。我們使用氘核磁共振（2H NMR）研究植物固醇對POPE人造細胞膜的物理特性之影響。其方法是先將POPE上sn-1的氫代換成氘，再將其混合不同濃度的植物固醇而成多個樣本，並將各樣本隨溫度改變量測得一系列光譜。 M1(T)值顯示，植物固醇對於gel態及lc態POPE膜的有序程度之影響是不一樣的：植物固醇對gel態膜的有序程度沒有明顯的改變；然而，它會增加lc態POPE膜的有序度，但此影響在植物固醇為20 mol%時達到飽和。此外，分析NMR光譜可得到溫度-濃度相圖，可觀察到兩個兩項共存區。

摘要(英)

Cholesterol and other sterols are important for the formation of lipid raft. It is now generally agreed that raft domains are in the liquid-ordered (lo) phase. Previous research on model membranes has found that sterol induces the formation of the lo-phase in lipid bilayers. While most studies were conducted in phosphatidylcholine lipid bilayers, fewer were conducted in phosphoethanolamine (PE) lipid biayers. To further understand the raft formation, exploring the interaction of sterol and PE lipid is necessary.We investigate the effect of phytosterol on the physical properties of 1-[2H31]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance(2H NMR). The sn-1 chain of POPE was deuterium labeled. 2H NMR spectra were taken as a function of temperature andphytosterol concentration. The spectral first moments show that the ordering effects of phytosterol on the gel-phase and liquid crystalline (lc)-phase POPE membranes are different: It has no significant effect on the membrane order in the gel phase.In contrast, it increases the chain order of lc-phase POPE,but this effect saturates at 20 mol% phytosterol.Moreover, the temperature-composition phase diagram, established from the NMR spectra, is presented. Two two-phase coexistence regions were observed.

關鍵字(中)

★ 細胞膜
★ 脂質筏
★ 膜相圖

關鍵字(英)

★ cell membrane
★ lipid rafts
★ phase diagram

論文目次

目錄

提要 I
英文提要 II
致謝 III
目錄 IV
圖目 VI
表目 IX
第一章緒論 1
1.1 生物膜概述 1
1.2 細胞膜結構 1
1.3 脂質分子 3
1.4 膜的相行為 5
1.5POPE與β-sitosterol 7
第二章實驗方法及材料 12
2.12H NMR原理 12
2.1.1共振原理 12
2.1.2 電四極交互作用 14
2.1.3 粉狀光譜(Powder Spectrum) 15
2.1.4 特徵光譜 17
2.1.5 First Moment 的定義 18
2.2 NMR硬體介紹及參數設定 20
2.3 樣本配置 20
第三章結果分析與討論 22
3.1POPE/β-sitosterol生物膜 22
3.2 POPE-d31／β-sitosterol膜的溫度-濃度相圖 36
第四章結論 38
參考文獻 40

參考文獻

參考文獻

[1] Matthew P. Scottet al., Molecular Cell Biology, 5th ed., W. H. Freeman, 2003.
[2] S. J. Singer, Garth L. Nicolson, The fluid mosaic model of the structure of cell membranes, Science, New Series, Vol. 175, No. 4023 (Feb. 18, 1972), pp. 720-731.
[3] Kai Simons, Elina Ikonen, Functional rafts in cell membranes, Nature 387, 569-572 (5 June 1997).
[4] Sangiorgio, V., Pitto, M., Palestini, P., & Masserini, M.E. (2004), GPI-anchored proteins and lipid rafts., The Italian journal of biochemistry, 53(2), 98-111.
[5] M. Edidin, The state of lipid rafts: from model membranes to cells, Annu. Rev. Biophys. Biomol.Struct. 2003. 32:257–83
[6] D.A. Brown, E. London, Structure and function of sphingolipid-and cholesterol-rich membrane rafts, Journal of Biological Chemistry 275 (2000) 17221-17224.
[7]E. Endress, S. Bayerl, K. Prechtel, C. Maier, R. Merkel, T.M. Bayerl, The effect ofcholesterol, lanosterol, and ergosterol on lecithin bilayer mechanicalproperties at molecular and microscopic dimensions: a solid-state NMR andmicropipet study, Langmuir 18 (2002)3293-3299.
[8] M. Luckey, Membrane structural biology: with biochemical and biophysicalfoundations, Cambridge Univ Pr, 2008.
[9] Philip L. Yeagle, The Structure of Biological Membranes, 3rd Ed., July 18, 2011 by CRC Press
[10]J. Eisenblätter, R. Winter, Pressure Effects on the structure and phase behavior of DMPC-gramicidin lipid bilayers: a synchrotron SAXS and 2H-NMR spectroscopy study, Biophysical journal 90 (2006) 956-966.
[11] S.L. Veatch, S.L. Keller, Organization in lipid membranes containing cholesterol,Physical review letters 89 (2002) 268101.
[12] J. Rubenstein, B.A. Smith, H.M. McConnell, Lateral diffusion in binary mixtures ofcholesterol and phosphatidylcholines, Proceedings of the National Academyof Sciences 76 (1979) 15.
[13] Shaikh SR, Brzustowicz MR, Gustafson N, Stillwell W, Wassall SR., Monounsaturated PE does not phase-separate from the lipid raft molecules sphingomyelin and cholesterol: role for polyunsaturation?, Biochemistry, 2002, 41 (34), pp 10593–10602.
[14] Löffler J, Einsele H, Hebart H, Schumacher U, Hrastnik C, Daum G., Phospholipid and sterol analysis of plasma membranes of azole-resistant Candida albicans strains., FEMS Microbiol Lett. 2000 Apr 1;185(1):59-63.
[15] Philip L. Ywagle, The Structure of Biological Membranes, 2nd ed., CRC PRESS.
[16] Md. Arif Kamal, V. A. Raghunathan, Phase behavior of phospholipid–phytosterol membranes, Soft Matter, 2012, 8, 8952
[17] Lees AM, Mok HY, Lees RS, McCluskey MA, Grundy SM, Plant sterols as cholesterol-lowering agents: clinical trials in patients with hypercholesterolemia and studies of sterol balance., Atherosclerosis. 1977 Nov;28(3):325-38.
[18] Ya-Wei Hsueh et al.,The effect of ergosterol on dipalmitoylphosphatidylcholine bilayers: a deuterium NMR and calorimetric study.,Biophys J. 2005Mar;88(3):1799-808. Epub 2004 Dec 13.
[19] Ya-Wei Hsueh et al.,Ergosterol in POPC membranes: physical properties and comparison with structurally similar sterols.,Biophys J. 2007 Mar 1;92(5):1606-15. Epub 2006 Dec 1.
[20] Davis JH, Schmidt ML., Critical behaviour in DOPC/DPPC/cholesterol mixtures: static (2)H NMR line shapes near the critical point., Biophys J. 2014 May 6;106(9):1970-8. doi: 10.1016/j.bpj.2014.03.037.
[21] Chantal Pare´ and Michel Lafleur, Polymorphism of POPE/Cholesterol System: A 2H Nuclear MagneticResonance and Infrared Spectroscopic Investigation, Biophysical Journal Volume 74 February 1998 899–909
[22] Ya-Wei Hsuehet al., Deuterium NMR Study of the Effect of Ergosterol on POPE Membranes, Biophysical Journal Volume 98 April 2010 1209–1217
[23] Jing-shian Wang, The physical properties of phytosterol-containing lipid bilayers.(王靖賢的碩論)
[24] M.M.C. David L. Nelson, Lehninger Principles of Biochemistry, Fourth Edition, 4ed., W. H. Freeman, 2004.
[25] http://www.perkepi.com/cell-membrane/
[26] http://www.reemazeineldin.com/Liposome.html
[27] Ilya Koltoveret al., Science 3 July 1998: Vol. 281 no. 5373 pp. 78-81

指導教授

薛雅薇(Ya-Wei Hsueh)

審核日期

2014-7-29

推文