博碩士論文 992204024 詳細資訊

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姓名 葉昱宏(Yu-Hung Yeh)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 台灣海邊耐鹽植物濱刺草之內生真菌
(Endophytic fungi from the salt-tolerant coast plant Spinifex littoreus in Taiwan)
★ 有益微生物的真菌學和細菌學研究: 在農業和人類健康中的應用★  Fungi on leaves of Nelumbo nucifera Gaertn.
★ 台灣白粉菌分類★ 北台灣腐生絲孢菌綱之採集與描述
★ 結合依賴培養法、不依賴培養法與伺機採集之方法研究台灣沿海植物濱刺草之內生與非內生真菌★ 仙草的可培養性內生真菌之多樣性
★ Selected fungicolous Hypocreales in northern Taiwan★ Fungi on banana in northern Taiwan
★ 野生植物的白粉菌鑑定在北台灣★ 台灣海岸植物之內生真菌多樣性研究
★ Classification of powdery mildews on ornamental plants in northern Taiwan★ Brevibacterium sp. TX4 與 Pseudomonas nitroreducens TX1 異化辛基苯酚聚氧乙基醇及其代謝物之生物分解途徑研究
★ 多種方法鑑定皮膚和指甲上真菌多樣性於台灣北部★ 藉由從環境中分離的真菌進行內分泌干擾物辛基酚 之生物降解與其預測之代謝途徑
★ 台灣沉香樹相關的內生菌
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摘要(中) 在過去內生真菌被認為是只存在植物體內而不會對植物造成影響。當植物死去時,內生真菌可以首先腐生死去的植物。在2008年,科學家證明了一種濱海的草本植物需要內生真菌與其共生才能夠抵抗鹽分逆境。此外這些內生真菌有廣泛的宿主範圍包含了單子葉與雙子葉植物。濱刺草是一種濱海植物廣泛生長在台灣海灘沙丘上。這種植物能夠忍受高鹽分、風沙侵襲、低水分與營養缺乏的環境。我們在台灣各地的海灘採集濱刺草,然後將植物的根、莖、葉片、葉鞘用95%乙醇和6%次氯酸鈉進行表面消毒,然後再用無菌水清洗。表面消毒的有效性用imprint technique來驗證。我們全部從3648個植物片段中獲得了1670株菌株。從濱刺草中分離的全部1670株菌株中可被分為59個種類。從植物組織中分離的內生真菌利用菌種型態顯微鏡觀察以及DNA分析來鑑定種類。這些種類對於這種植物來說是新的紀錄,其中還有一種是尚未被發現的新種類Sarocladium sp.。
摘要(英) In the past, endophytic fungi were considered just living in plants without causing impact to the plant. When the plants die, the endophytic fungi can first grow saprobically in dead plants. In 2008, scientists demonstrated that a coastal grass species requires symbiotic fungal endophytes for salt tolerance. In addition, these endophytes have a broad host range encompassing both monocots and eudicots. Spinifex littoreus is a coastal plant in littoral sand dunes common in tropical Asia. The grass is able to tolerate high salinity, sand movement, low water and nutrient availability. We collected Spinifex littoreus at different places in Taiwan. Spinifex littoreus was divided into root, stem, leaf sheath and leaf lamina. Plants were surface-sterilized with 95% ethanol and 6% sodium hypochlorite and then rinsed in sterile water. The effectiveness of surface sterilization was verified by the imprint technique. From 3648 plant segments, altogether 1670 cultures comprising 59 species of fungi were isolated from S. littoreus. Endophytes were isolated from plant tissues and identified based on culture morphology, light microscopy and DNA analysis. The species were all recorded for this plant for the first time worldwide. An undescribed species of Sarocladium was discovered and characterized.
關鍵字(中) ★ 內生真菌
★ 台灣
★ 濱刺草
關鍵字(英) ★ endophytic fungi
★ Taiwan
★ Spinifex littoreus
論文目次 目錄       
中文摘要 i
英文摘要 ii
誌謝 iii
目錄 iv
圖目錄 vi
表目錄 vii
1 Introduction 1
2 Materials and methods 3
2-1 Plants and collection sites 3
2-2 Isolation of fungi 3
2-3 Surface sterilization 4
2-4 Media 4
2-5 DNA isolation 5
2-6 PCR 8
2-7 Agarose gel electrophoresis 10
2-8 Purification of PCR products 10
2-9 DNA sequencing 11
2-10 Morphological and molecular identification 11
2-11 Isolation frequency and colonization rate 12
2-12 Statistical analysis 12
3 Results 13
3-1 Comparison of fungal composition between MEA and SNA 13
3-2 Species richness of endophytic fungi 13
3-3 Comparison of endophytic species composition among the plant organs 13
3-4 Comparison of endophytic species composition among sampling sites 14
3-5 Description of species 15
3-5-1 Alternaria alternata 15
3-5-2 Alternaria tenuissima 16
3-5-3 Aspergillus terreus 17
3-5-4 Fusarium oxysporum 18
3-5-5 Microsphaeropsis arundinis 19
3-5-6 Moniliophthora sp. 20
3-5-7 Phaeosphaeriopsis sp. 21
3-5-8 Rhizoctonia solani 22
3-5-9 Sarocladium sp. nov. 33
4 Discussion 25
5 References 29
參考文獻 陳明義 1991海岸植物資源的保育 科學月刊 0264 900-903
伍淑惠, 張元聰, 高瑞卿 2010台灣海濱植物圖鑑,台北
廖月華和吳波明 2000水稻紫鞘病病因研究分析 江西農業大學學報 22:62-67
Aime M.C. & Phillips-Mora W. 2005 The causal agents of witches’ broom and frosty pod rot of cacao (chocolate, Theobroma cacao) form a new lineage of Marasmiaceae Mycologia, 97(5): 1012-1022.
Ananda K. & Sridhar K.R. 2002 Diversity of endophytic fungi in the roots of mangrove species on the west coast of India Can J Microbiol.48(10):871-8.
Arzanlou M. & Crous P.W. 2006 Fungal Planet, A Global Initiative to Promote the Study of Fungal Biodiversity 9: 1.
Beena K.R., Arun A.B., Raviraja N.S. & Sridhar K.R. 2001 Association of arbuscular mycorrhizal fungi with plants of coastal sand dunes of west coast of India Tropical Ecology 42(2): 213-222.
Domsch K.H., Gams W. & Anderson T.-H. 2007 Compendium of Soil Fungi. Second edition. IHW-Verlag, Eching.
Farr D.F. & Rossman A.Y. 2012 Fungal Databases, Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved October 15, from http://nt.ars-grin.gov/fungaldatabases
Felsenstein J. 1985 Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
Gardes M. & Bruns T.D. 1993 ITS primers with enhanced specifity for Basidiomycetes: application to identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.
Gasoni L. & Gurfinkel B. S. 2009 Biocontrol of Rhizoctonia solani by the endophytic fungus Cladorrhinum foecundissimum in cotton plants Australasian Plant Pathology 38:
GenBank (www.ncbi.nlm.nih.gov/blast/Blast.cgi?CMD=Web&PAGE_TYPE=BlastHome)
Godinho A., Ramesh R. & Bhosle S. 2010 Bacteria from Sand Dunes of Goa Promoting Growth in Eggplant Agricultural Sciences 6 (5): 555-564.
Gonzalez D., Carling D.E., Kuninaga S., Vilgalys R. & Cubeta M.A. 2001 Ribosomal DNA systematics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs Mycologia 93: 1138-1150
Hawksworth D. L. 1990 The fungal dimension of biodiversity: magnitude, significance, and conservation. Mycol. Res. 95: 641-655.
Hawksworth D. L. 2001 The magnitude of fungal diversity: the 1±5 million species estimate revisited Mycol. Res. 105 (12): 1422-1432.
Huckelhoven R., Neumann C., Wettstein D.V., Franken P., & Kogel K.H. 2005 The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. PNAS 102 38: 13386-13391.
Index Fungorum (www.indexfungorum.org/names/names.asp)
Jabal L.R. & Vidal S. 2010 Fungal endophyte negative effects on herbivory are enhanced on intact plants and maintained in a subsequent generation. Ecological Entomology 35: 25-36.
Kerekes J.F. & Desjardin D.E. 2009 A monograph of the genera Crinipellis and Moniliophthora from Southeast Asia including a molecular phylogeny of the nrITS region. Fungal Diversity 37: 101-152.
Krings M., Taylor T.N., Hass H., Kerp H., Dotzler N., Hermsen E.J. 2007 Fungal endophytes in a 400-million-yr-old land plant: infection pathways, spatial distribution, and host responses. New Phytol 174:648-657.
Lee H.B., Kim K.M. & Jung H.S. 2005 Paraphaeosphaeria recurvifoliae, a new species causing leaf spots and necrosis on Yucca recurvifolia. Fungal Diversity 20: 71-81.
Márquez L.M., Redman R.S., Rodriguez R.J., Roossinck M.J. 2007 A Virus in a Fungus in a Plant: Three-Way Symbiosis Required for Thermal Tolerance Science 315: 513-515.
Nirenberg H.I. 1976 Untersuchungen u¨ber die morphologische und biologische Differenzierung in der Fusarium-Section Liseola. Berlin-Dahlem: Mitt Biol Bundesanst fu¨r Land- und Forstwirt. 169: 1-117.
Orole O.O. & Adejumo T.O. 2009 Activity of fungal endophytes against four maize wilt pathogens. African Journal of Microbiology Research 3(12): 969-973.
Otero J.T., Ackerman J.D. & Bayman, P. 2002 Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids. American Journal of Botany 89(11): 1852-1858.
Rakeman J.L., Bui U., Lafe K., Chen Y.C., Honeycutt R.J. & Cookson B.T. 2005 Multilocus DNA sequence comparisons rapidly identify pathogenic molds Microbiol. 43 (7), 3324-3333.
Ramaley A.W. 1997 New Paraphaeospheria and their anamorphs Mycotaxon LXI: 347-358.
Ramaley A.W., Castlebury L.A. & Palm M.E. 2003 Neophaeosphaeria and Phaeosphaeriopsis, segregates of Paraphaeosphaeria. Mycological Research 107: 516-522.
Redman R.S., Sheehan K.B., Stout R.G., Rodriguez R.J., Henson J.M. 2002 Thermotolerance Generated by Plant/Fungal Symbiosis. Science 298: 22.
Renker C., Otto P., Schneider K., Zimdars B., Maraun M. & Buscot, F. 2005 Oribatid mites as potential vectors for soil microfungi: study of mite-associated fungal species Microb. Ecol. 50 (4), 518-528.
Rodrigues A., Bacci M., Mueller U.G., Ortiz A., Pagnocca F.C. 2008 Microfungal ‘weeds’ in the leafcutter ant symbiosis. Microb Ecol 56:604-614.
Rodriguez R.J., Henson J., Volkenburgh E.V., Wright M.H., Beckwith F., Kim Y.O., &
Redman R.S. 2008 Stress tolerance in plants via habitat-adapted symbiosis. The ISME Journal 2: 404-416.
Saitou N. & Nei M. 1987 The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
Sánchez Márquez S., Bills G.F. & Zabalgogeazcoa I. 2008 Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Diversity 33: 87-100.
Schardl C.L., Leuchtmann A. & Spiering, M.J. 2004 Symbioses of grasses with seedborne fungal endophytes. Annual Review of Plant Biology 55: 315-340.
Selim K.A., El-Beih A.A., AbdEl-Rahman T.M. & El-Diwany AI. 2011 Biodiversity and antimicrobial activity of endophytes associated with Egyptian medicinal plants. Mycosphere 2(6): 669-678.
Simmons E.G. 2007 Alternaria: an identification manual. CBS Fungal Biodiversity Centre, The Netherlands, Utrecht.
Sun Y., Wang Q., Lu X., Okane I. & Kakishima M. 2012 Endophytic fungal community in stems and leaves of plants from desert areas in China Mycological Progress 11, Number 3 : 781-790.
Sutton B.C. 1980 The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, England.
Tamura K., Nei M., & Kumar S. 2004 Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences 101: 11030-11035.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., & Kumar S. 2011 MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28 (10): 2731-2739.
Tzean S.S., Chen J.L., Liou G.Y., Chen C.C., Hsu W.H. 1990 Aspergillus and related teleomorphs from Taiwan. Food Industry Research and Development Institute, Hsinchu, Taiwan.
Waller F., Achatz B., Baltruschat H., Fodor J., Becker K., Fischer M., Heier T.,
Summerbell R.C., Gueidan C., Schroers H-J., de Hoog G.S., Starink M., Arocha Rosete Y., Guarro J. & Scott J.A. 2011 Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium, and Trichothecium. Studies in Mycology 68: 139-162.
Weiß M., Yang Z.L. & Oberwinkler F. 1998 Molecular phylogenetic studies in the genus Amanita. Can. J. Bot. 76: 1170-1179.
White T.J., Bruns T.D., Lee S. & Taylor J. 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. eds. PCR protocols, a guide to methods and applications. San Diego, California: Academic Press. 315-322.
Yarita K., Sano A. and Une Y. 2010 An atypical genotype of Cladophialophora carrionii isolated from a pacific white-sided dolphin (Lagenorhynchus obliquidens) Published Only in Database.
指導教授 羅南德(Roland Kirschner) 審核日期 2013-1-25
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