博碩士論文 992213006 詳細資訊




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姓名 林婷婷(Ting-Ting Lin)  查詢紙本館藏   畢業系所 系統生物與生物資訊研究所
論文名稱 探討牛樟芝CCM111對細胞訊息傳遞之影響
(The Study of CCM111 in Cell Signaling Transduction Pathways)
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摘要(中) 牛樟芝,是台灣菌類特有種。一直以來被台灣原住民作為解酒以及治療肝病
的中草藥。有研究報導指出,牛樟芝萃取物具有抗肝炎病毒的效果並且能夠抑制
肝癌的形成。
訊息傳遞控制了細胞的功能,如細胞增生、分化等。而我們知道,許多癌症的產生與訊息傳遞的異常表現有關係。因此欲建立訊息傳遞平台,進行對牛樟芝的全面性篩選,進而得知牛樟芝能透過調控哪一條訊息傳遞而達到抗發炎反應甚至抗腫瘤生長的功能,為我實驗的主要目的。
摘要(英) CCM111, an extraction of Antrodia camphorate (AC) is a genus of fungi in Taiwan. It is well known as a Traditional Chinese Medicine (TCM) and has been widely used during Taiwanese aborigines as a drug for treating hangover and liver diseases.
In my study, we have collected stable cell lines expressing transcriptional regulatory elements in front of luciferase reporter to monitor activity of the signaling pathways.
關鍵字(中) ★ 發炎反應
★ 中草藥
★ 牛樟芝
★ 訊息傳遞
關鍵字(英) ★ inflammation
★ signaling transduction pathway
★ Antrodia camphorate
★ Traditional chinese medicine
論文目次 摘要 . i
Abstract ii
誌謝 iii
Table of contents . iv
List of figures. vi
List of tables . viii
I. INTRODUCTION . 1
1. Antrodia camphorata . 1
2. Signaling transduction pathway 2
3. Inflammation-related signaling transduction pathway 2
4. Cytokine-related signaling transduction pathway . 4
5. Hormone related signaling transduction pathway 6
II. MATERIAL AND METHOD . 8
1. Materials . 8
1.1 Chemicals . 8
1.2 Antibodies . 8
1.3 Cell lines . 8
1.4 Stable clones with TRE luciferase reporters 9
1.5 pGL4.20 [luc2/Puro] . 9
1.6 Restriction enzymes 9
1.7 Primer sequences 9
1.8 DNA ligation enzyme . 10
2. Method 10
2.1 Establishment of stable clone expressing TRE luciferase reporter 10
v
2.2 Luciferase assay 10
2.3 Transformation . 11
2.4 Plasmid extraction 11
2.5 Transfection 11
2.6 SDS-PAGE and Western blot analysis . 11
2.7 Alamar blue assay . 12
III. RESULTS 13
1. Establishment of the luciferase assay platform for screening signaling
transduction pathways . 13
2. Establishment of Nrf2 luciferase reporter cell line . 13
3. Establishment of FOXO luciferase reporter cell line 14
4. Establishment of ELK-1 luciferase reporter cell line . 14
5. Titration of established luciferase reporter cell lines 15
6. The effect of CCM111 in signaling transduction pathways . 16
7. The toxicity of CCM111 in human mononuclear cells . 16
8. The effect of CCM111 in STAT3 signaling pathway . 17
9. Cell proliferation rate for CCM111 on colon cancer cell lines. 17
IV. DISCUSSION . 18
1. The effect of CCM111 in signaling transduction pathways . 18
2. CCM111 plays anti-inflammatory role . 19
3. Other functions of Antrodia camphorata 19
4. Future aspect . 20
V. REFERENCES . 21
FIGURES 23
TABLES . 55
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23. Rao, Y.K., et al., Identification of Antrocin from Antrodia camphorata as a Selective and Novel Class of Small Molecule Inhibitor of Akt/mTOR Signaling in Metastatic Breast Cancer MDA-MB-231 Cells. Chem Res Toxicol, 2010. 24(2): p. 238-245.
指導教授 馬念涵(Nianhan Jia-Lin Ma) 審核日期 2012-8-24
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