兩性雙離子性共聚物之合成以抵抗聚丙烯膜表面之生物積垢

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王裕貴(Yu-Kuei Wang) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

兩性雙離子性共聚物之合成以抵抗聚丙烯膜表面之生物積垢
(Amphiphilic and zwitterionic copolymer synthesis for anti-fouling surface coating on polypropylene membranes)

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摘要(中)

雙離子性(zwitterionic)材料為一同時具有正電性電荷及負電性電荷於同一單體而成一電中性的結構，已被證實具有良好的抗生物積垢能力，但不易改質至疏水性材料表面，因此本篇研究擬合成一同時具有疏水性片段與雙離子性結構片段的共聚物，使其疏水性片段得以利用疏水性作用力附著於疏水性聚丙烯(polypropylene, PP)濾膜上，暴露在外的雙離子性結構則用以對抗生物分子的沾黏。本研究選用順丁烯二酸酐(Maleic anhydride, MA)，使之與疏水性單體丙烯酸十八酯(octadecyl acrylate, OA)及丙烯酸己酯(hexyl acrylate, HA)於共溶劑下共聚合，再將所得之共聚物再以N,N’-二甲基乙二胺(N,N’-Dimethylethylenediamine, DMEA)開環而形成一同時具有疏水性片段與雙離子性結構片段的共聚物(MAOA-DMEA與MAHA-DMEA)，並透過控制不同的共聚合起始劑量以獲得不同分子量共聚物，且藉由核磁共振光譜儀(1H-NMR)分析其化學結構，及以膠體滲透層析儀(GPC)分析其分子量。接著量測經過不同分子量的共聚物改質之PP濾膜對牛血清蛋白(bovine serum albumin, BSA)溶液中的蛋白質吸附變化量，並觀察測試細菌E. coli與S. maltophilia在經共聚物改質後的PP膜上貼附的情形。實驗結果發現經過MAOA-DMEA系列改質的PP緻密膜可降低80%以上的BSA吸附量，且對抵抗E. coli與S. maltophilia的貼附具有一定的能力。雙離子性(zwitterionic)材料為一同時具有正電性電荷及負電性電荷於同一單體而成一電中性的結構，已被證實具有良好的抗生物積垢能力，但不易改質至疏水性材料表面，因此本篇研究擬合成一同時具有疏水性片段與雙離子性結構片段的共聚物，使其疏水性片段得以利用疏水性作用力附著於疏水性聚丙烯(polypropylene, PP)濾膜上，暴露在外的雙離子性結構則用以對抗生物分子的沾黏。本研究選用順丁烯二酸酐(Maleic anhydride, MA)，使之與疏水性單體丙烯酸十八酯(octadecyl acrylate, OA)及丙烯酸己酯(hexyl acrylate, HA)於共溶劑下共聚合，再將所得之共聚物再以N,N’-二甲基乙二胺(N,N’-Dimethylethylenediamine, DMEA)開環而形成一同時具有疏水性片段與雙離子性結構片段的共聚物(MAOA-DMEA與MAHA-DMEA)，並透過控制不同的共聚合起始劑量以獲得不同分子量共聚物，且藉由核磁共振光譜儀(1H-NMR)分析其化學結構，及以膠體滲透層析儀(GPC)分析其分子量。接著量測經過不同分子量的共聚物改質之PP濾膜對牛血清蛋白(bovine serum albumin, BSA)溶液中的蛋白質吸附變化量，並觀察測試細菌E. coli與S. maltophilia在經共聚物改質後的PP膜上貼附的情形。實驗結果發現經過MAOA-DMEA系列改質的PP緻密膜可降低80%以上的BSA吸附量，且對抵抗E. coli與S. maltophilia的貼附具有一定的能力。

摘要(英)

Zwitterionic moieties that simultaneously own a positively and a negatively charged groups have been demonstrated to have excellent anti-fouling ability. The remaining problem is how to attach zwitterionic moieties onto the surface of a hydrophobic membrane, such as the polypropylene (PP) membrane. To solve this problem, we try to synthesize amphiphilic zwitterionic copolymers, which compromise both hydrophobic and zwitterionic monomers, onto the PP membranes. Zwitterionic monomers are highly water soluble. It is difficult to find a suitable solvent which simultaneously dissolve hydrophobic and zwitterionic monomers. Therefore, we try to synthesize the copolymers by co-polymerize octadecyl acrylate or hexyl acrylate with maleic anhydride in toluene, and then the maleic anhydride in copolymers are reacted with N,N’-Dimethylethylenediamine (DMEA) to form a zwitterionic moiety containing both carboxylic and N,N’-dimethylethyleneamine. The structures and molecular weight of copolymers have been characterized by NMR and GPC. The effect of molecular weight of copolymers on the antifouling properties of coated membranes are under investigation. The amount of BSA adsorption and bacteria (E. coli and S. maltophilia) adhesion on PP membranes are measured. The experimental results show that the PP dense membrane which modified through the MAOA-DMEA series copolymer can reduce more than 80% of BSA adsorption, and they have certain ability to resist the adhesion of S. maltophilia.

關鍵字(中)

★ 丙烯酸酯
★ 馬來酸酐
★ 抗生物積垢
★ 聚丙烯膜
★ 雙離子性

關鍵字(英)

★ Zwitterionic
★ Polypropylene membrane
★ Anti-fouling
★ Maleic anhydride
★ Acrylate

論文目次

摘要 I
Abstract II
誌謝 III
目錄 IV
圖目錄 VII
表目錄 XIV
第一章緒論 1
1.1 研究動機 1
1.2 研究目的 3
第二章文獻回顧 4
2.1 聚丙烯膜（Polypropylene, PP）簡介 4
2.2 生物性分子與材料表面之交互作用 4
2.2.1 生物積垢(Bio-fouling) 4
2.2.2 蛋白質與材料表面之交互作用 6
2.2.3 細菌與材料表面之交互作用 9
2.3 抗生物積垢材料之簡介 12
2.3.1 聚乙烯乙二醇之簡介與發展 12
2.3.2 仿生雙離子性高分子相關研究 15
2.3.2.1 Phosphorylcholine,PC類雙離子性高分子 16
2.3.2.2 Sulfobetaine,SBMA類雙離子高分子 19
2.3.2.3 Carboxybetaine, CBMA類雙離子高分子 27
2.4 共聚物簡介與表面改質方法 31
2.4.1 共聚物簡介 31
2.4.2 表面改質方法 33
第三章實驗藥品、儀器設備與方法 36
3.1 實驗藥品 36
3.2 實驗儀器設備 38
3.3 實驗方法 39
3.3.1 實驗架構 39
3.3.2 雙離子性高分子(MAOA-DMEA)共聚物的製備 39
3.3.2.1 共聚物(MAOA)的製備 39
3.3.2.2 兩性雙離子性共聚物(MAOA-DMEA)的製備 40
3.3.3 雙離子性共聚物(MAHA-DMEA)高分子的製備 41
3.3.3.1 共聚物(MAHA)的製備 41
3.3.3.2 雙離子性共聚物(MAHA-DMEA)的製備 41
3.3.4 MAOA-DMEA共聚物分子量鑑定 42
3.3.5 MAHA-DMEA共聚物分子量鑑定 42
3.3.6 MAOA-DMEA共聚物結構鑑定 42
3.3.7 MAHA-DMEA共聚物結構鑑定 43
3.3.8 PP膜-f表面改質 43
3.3.8.1 PP膜-f表面改質前清洗 43
3.3.8.2 MAOA-DMEA共聚物改質PP膜-f表面 43
3.3.8.3 MAHA-DMEA共聚物改質PP膜-f表面 44
3.3.8.4 MAO-DMEA共聚物改質PP膜-f表面 44
3.3.8.5 SMA-DMEA共聚物改質PP膜-f表面 45
3.3.8.6 PP膜-m表面改質 46
3.3.8.7 PP膜-m表面改質前清洗 46
3.3.8.8 MAOA-DMEA共聚物改質PP膜-m表面 46
3.3.8.9 MAHA-DMEA共聚物改質PP膜-m表面 47
3.3.8.10 MAO-DMEA共聚物改質PP膜-m表面 47
3.3.8.11 SMA-DMEA共聚物改質PP膜-m表面 48
3.3.9 改質後PP膜之表面鑑定 49
3.3.10 螢光標記蛋白質(BSA-FITC)的製備 49
3.3.11 螢光標記蛋白質(BSA-FITC)吸附實驗 49
3.3.11.1 PP膜-f的螢光標記蛋白質吸附實驗 49
3.3.11.2 PP膜-m的螢光標記蛋白質吸附實驗 50
3.3.12 改質後PP膜-f穩定性實驗 50
3.3.13 E.coli貼附實驗 51
3.3.13.1 E.coli菌液液態培養 51
3.3.13.2 改質後PP膜-f的E.coli貼附實驗 51
3.3.14 S.epidermidis貼附實驗 52
3.3.14.1 S.epidermidis菌液液態培養 52
3.3.14.2 改質後PP膜-f的S.epidermidis貼附實驗 52
第四章結果與討論 53
4.1 兩性雙離子性共聚物製備 53
4.2 兩性雙離子性共聚物分子量鑑定 55
4.3 兩性雙離子性共聚物結構鑑定 56
4.4 兩性雙離子性共聚物在PP膜上的改質 68
4.5 螢光蛋白質(BSA-FITC)吸附實驗 70
4.5.1 BSA-FITC在改質PP膜上-f的吸附實驗 71
4.5.2 BSA-FITC在改質PP膜-m上的吸附實驗 74
4.6 革蘭氏細菌在改質後PP膜-f的貼附實驗 77
第五章結論 80
第六章參考文獻 81
附錄一 85
附錄二 86

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指導教授

阮若屈、張雍
(Ruoh-chyu Ruaan、Yung Chang)

審核日期

2012-8-1

推文