Galectin-1誘導纖維母細胞於幾丁聚醣膜
上增生之訊號傳遞機制研究

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胡明惠(Ming-Hui Hu) 查詢紙本館藏

畢業系所

生命科學系

論文名稱

Galectin-1誘導纖維母細胞於幾丁聚醣膜上增生之訊號傳遞機制研究
(Signaling mechanism of Galectin-1 induced 3T3 cell proliferation on chitosan membrane )

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摘要(中)

幾丁聚醣是一種天然無毒性的高分子，具有生物可分解性及生物相容性。近幾年來，幾丁聚醣所製成的薄膜已在臨床醫學上作為生物敷料。而Galectin-1(GAL1)是一種β半乳糖苷結合的蛋白質，在於調節細胞的貼附、發育與調控細胞的生長。本實驗室先前的研究指出: GAL1可以促進3T3 (纖維母細胞)在幾丁聚醣膜上增生。因此本論文中進一步探討GAL1誘使3T3細胞在幾丁聚醣膜上增生的機制。從實驗結果得知，GAL1對於3T3細胞的貼附及增生扮演很重要的角色。為了分析細胞增生的訊號傳遞機制，將3T3細胞處理Genistein(酪胺酸激活酶) 抑制劑與Na3VO4
(酪胺酸去磷酸酶抑制劑)，並將細胞培養於有GAL1吸附的幾丁聚醣膜上。結果顯示Na3VO4可以協力促進GAL1誘發3T3細胞於幾丁聚醣膜上的增生。然而，細胞單獨處理Genistein 或Na3VO4 對於細胞增生並無明顯差異。另外，以免疫沉澱法測定可得知GAL1誘使3T3細胞增生是透
過cdc2激活酶的活性增加所導致。此外，我們實驗也證實GAL1促使細胞增生是藉由ERK1/2訊號傳遞路徑所調控。綜合本論文實驗結果，我們證實GAL1誘使3T3細胞在幾丁聚醣膜增生的機制是透過ERK1/2的訊號傳遞路徑及cdc2激活酶的活化。

摘要(英)

Chitosan is considered to be a very promising biopolymer for various biomedical and pharmaceutical uses because of its nontoxic and
biocompatible natures. In clinical medicine, chitosan membrane has been used as a biological dressing. Galectin-1 (GAL1), a β-galactoside-binding protein, functions in cell adhesion, development, and growth regulation. Previous study in this laboratory have shown that GAL1 support 3T3 proliferation on chitosan membrane. In this study, we further investigated the mechanism of GAL1-induced 3T3 cell proliferation on chitosan membrane. The results show that GAL1 play an important role in enhancing 3T3 cell adhesion and proliferation on the surface of chitosan membrane. To better characterize a plausible signaling pathway, the 3T3 cells were pre-treated with various kinase or phosphatase inhibitors and cultured in a GAL1-coated chitosan membrane. The results show that Na3VO4 (tyrosine phosphatase inhibitor) can synergistically enhance
GAL1-induced 3T3 cell proliferation on chitosan membrane. In contrast,genistein (tyrosine kinase inhibitor) and Na3VO4 had no dramatic effect on
the 3T3 seeded on culture plates. Moreover, immunoprecipitation assay indicates that cdc2 kinase activity is significantly enhanced in 3T3 cells growing on GAL1-coated culture plates. The current results also suggest that GAL1 induces 3T3 cells proliferation through the ERK1/2 signal
pathway. Altogether, we proposed that the signal mechanism of GAL1-induced 3T3 proliferation on chitosan membrane was mediated through ERK1/2 and cdc2 kinase.

關鍵字(中)

★ 幾丁聚醣膜
★ 組織工程
★ 細胞增生
★ 訊號傳遞

關鍵字(英)

★ chitosan
★ tissue engineering
★ 3T3 cell
★ Galectin-1

論文目次

LIST OF CONTENTS ………………………………………I
LIST OF FIGURES ………………………………………IV
ABBREVIATION ………………………………………… VI
CHAPTER 1 INTRODUCTION ………………………………1
1. 1 Tissue engineering …………………………… 1
1. 2 Galectin-1 ……………………………………… 2
1. 3 Chitin and Chitosan ……………………………4
1. 4 Protein tyrosine phosphorylation ………… 7
1. 5 Mitogen-activated protein kinase (MAPK) cascade ………………………………………………… 8
1. 6 Cell cycle and cyclin B-CDK1 …………………………………………………………… 9
1. 7 Preliminary studies …………………………………………………………… 10
1. 8 Specific aim …………………………………… 11
CHAPTER 2 MATERIAS AND METHODS …………………………………………………………… 13
2. 1 Cell culture …………………………………… 13
2. 2 GAL1 protein purification ……………………13
2. 3 Coating of chitosan membrane with GAL1 proteins ……………………………………………… 14
2. 4 Cell attachment and proliferation on chitosan membrane …………………………………… 15
2. 5 Cell proliferation assay …………………………………………………………… 15
2. 6 Fluorescence-Activated Cell Sorter (FACS) analysis …………………………………………………16
2. 7 Western blot analysis …………………………17
2. 7. 1 Protein assay and Quantification …………………………………………………………… 17
2. 7. 2 Gel electrophoresis ……………………… 17
2. 7. 3 Western blot analysis………………………18
2. 8 Immunoprecipitation and CDC2 kinase assay …………………………………………………………… 18
2. 9 Fluorescence histochemistry analysis …………………………………………………………… 19
CHAPTER 3 RESULTS …………………………………… 21
3. 1 Effect of exogenous GAL1 on cell growth on chitosan membranes ………………………………… 21
3. 2 Immunohistochemical localization of GAL1 in 3T3 cells cultured on chitosan membranes …………………………………………………… …… 22
3. 3 Effects of Genistein and Na3VO4 on the growth of 3T3 cells ………………………………… 23
3. 4 Effects of Na3VO4 on GAL1-induced 3T3 cells growth on culture plates ………………………… 24
3. 5 Na3VO4 potentiates GAL1-induced 3T3 cell proliferation on chitosan membrane …………… 24
3. 6 Na3VO4 potentiates GAL1-induced 3T3 cell on G2/M progression …………………………………… 25
3. 7 Effect of Na3VO4 on GAL1-induced cyclin-B and cdc2 protein expression ……………………… 25
3. 8 Effects of Na3VO4 on GAL1-induced cdc2 activity in 3T3 cells……………………………… 26
3. 9 Signaling pathway involved in GAL1-induced 3T3 cells proliferation ………………………… 27
CHAPTER 4 DISCUSSION………………………………… 29
CHAPTER 5 REFERENCE………………………………… 49
APPENDIX ……………………………………………… 57

參考文獻

Abrieu, A., D. Fisher, et al. (1997). "MAPK inactivation is required for the G2 to M-phase transition of the first mitotic cell cycle." Embo J. 16: 6407-13.
Akiyama, S. K. (2002). "Functional analysis of cell adhesion: quantitation of cell-matrix attachment." Methods Cell Biol. 69: 281-96.
Alsarra, I. A., S. H. Neau, et al. (2004). "Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase." Biomaterials 25: 2645-55.
Bardford, M. A. (1976). "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the priciple of protein dye binding." Anal. Biochem. 72: 248-54.
Barondes, S. H., D. N. Cooper, et al. (1994). "Galectins. Structure and function of a large family of animal lectins." J. Biol. Chem. 269: 20807-10.
Becker, P. M., A. D. Verin, et al. (2001). "Differential regulation of diverse physiological responses to VEGF in pulmonary endothelial cells." Am. J. Physiol. Lung Cell Mol. Physiol. 281: L1500-11.
Berthod, F., D. Hayek, et al. (1993). "Collagen synthesis by fibroblasts cultured within a collagen sponge." Biomaterials 14: 749-54.
Bianchet, M. A., H. Ahmed, et al. (2000). "Soluble beta-galactosyl-binding lectin (galectin) from toad ovary: crystallographic studies of two protein-sugar complexes." Proteins 40: 378-88.
Bicknell, K. A., C. H. Coxon, et al. (2004). "Forced expression of the cyclin B1-CDC2 complex induces proliferation in adult rat cardiomyocytes." Biochem. J. 382: 411-6.
Borsch-Haubold, A. G., S. Pasquet, et al. (1998). "Direct inhibition of cyclooxygenase-1 and -2 by the kinase inhibitors SB 203580 and PD 98059. SB 203580 also inhibits thromboxane synthase." J. Biol. Chem 273: 28766-72.
Brooks, G., R. A. Poolman, et al. (1997). "Expression and activities of cyclins and cyclin-dependent kinases in developing rat ventricular myocytes." J. Mol. Cell Cardiol. 29: 2261-71.
Chang, Y. Y., S. J. Chen, et al. (2004). "The effect of galectin 1 on 3T3 cell proliferation on chitosan membranes." Biomaterials 25: 3603-11.
Chen, Q., M. S. Kinch, et al. (1994). "Integrin-mediated cell adhesion activates mitogen-activated protein kinases." J. Biol. Chem. 269: 26602-26605.
Cho, M. and R. D. Cummings (1995). "Galectin-1, a beta-galectoside-binding lectin in Chinease hamster ovary cells: I. Physical and chemical characterization." J. Biol. Chem. 270: 5198-5206.
Cindolo, L., G. Benvenuto, et al. (1999). "Galectin-1 and galectin-3 expression in human bladder transitional-cell carcinomas." INT. J. CANCER. 84: 39-43.
Clark, E. A. and J. S. Brugge (1995). "Integrins and signal transduction pathways: the road taken." Science 268: 233-239.
Clark, E. A. and R. O. Hynes (1996). "Ras activation is necessary for integrinmediated activation of extracellular signal-regulated kinase 2 and cytosolicphospholipase A2 but not for cytoskeletal organization." J. Biol. Chem. 271: 14814-14818.
Cleves, A. E., D. N. Cooper, et al. (1996). "A new pathway for protein export in Saccharomyces cerevisiae." J. Cell Biol. 133: 1017-26.
Cobb, M. H. (1999). "MAP kinase pathways." Prog. Biophys. Mol. Biol. 71: 479-500.
Cobb, M. H., A. E. Cleves, et al. (1991). "Extracellular signal-regulated kinases: ERKs in progress." Cell Regul. 2: 965-78.
Cooper, D. N. W. and S. H. Barondes (1990). "Evidence for export of a muscle lectin from cytosol to extracellular matrix and for anovel secretory." J. Cell Biol. 110: 1681-1691.
Denuziere, A., D. Ferrier, et al. (1998). "Chitosan-chondroitin sulfate and chitosan-hyaluronate polyelectrolyte complexes: biological properties." Biomaterials 19: 1275-85.
Ding, Z., J. G. Chen, et al. (2004). "Immobilization of chitosan onto poly-L-lactic acid film surface by plasma graft polymerization to control the morphology of fibroblast and liver cells." Biomaterials 25: 1059-1067.
Draetta, G. and D. Beach (1988). "Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement." Cell 54: 17-26.
Elad-Sfadia, G., R. Haklai, et al. (2004). "Galectin-3 augments K-Ras activation and triggers a Ras signal that attenuates ERK but not phosphoinositide 3-kinase activity." J. Biol. Chem. 279: 34922-30.
Elad-Sfadia, G., R. Haklai, et al. (2002). "Galectin-1 augments Ras activation and diverts Ras signals to Raf-1 at the expense of phosphoinositide 3-kinase." J. Biol. Chem. 277: 37169-75.
Flint, A. J., T. Tiganis, et al. (1997). "Development of substrate-trapping mutants to identify physiological substrates of protein tyrosine phosphatases." Proc. Natl. Acad. Sci. USA 94: 1680-1685.
Giancotti, F. G. (1997). "Integrin signaling: specificity and control of cell survival and cell cycle progression." Current Opinion in Cell Biology 9: 691-700.
Gingras, M., J. Bergeron, et al. (2003). "In vitro development of a tissue-engineered model of peripheral nerve regeneration to study neurite growth." Faseb J. 17: 2124-6.
Guay, J., H. Lambert, et al. (1997). "Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27." J. Cell Sci. 110: 357-68.
Harding, A., N. Giles, et al. (2003). "Mechanism of Mitosis-specific Activation of MEK1." J. Biol. Chem. 278: 16747-16754.
Hirabayashi, J. and K. Kasai (1993). "The family of metazoan metal-independent beta-galactoside-binding lectins: structure, function and molecular evolution." Glycobiology 3: 297-304.
Hubbard, S. R. (1999). "Structural analysis of receptor tyrosine kinases." Prog. Biophys. Mol. Biol. 71: 343-58.
Hutmacher, D. W., J. C. H. Goh, et al. (2001). "An introduction to biodegradable materials for tissue engineering applications." Ann. Acad. Med. Singapore 30: 183-91.
Hynes, M. A., L. B. Buck, et al. (1989). "Carbonhydrate recognition in neuronal development: structure, expression of surface oligosaccarides and beta-galactoside-binding lectins." Clin. Symp. 145: 189-210.
Joubert, R., M. Caron, et al. (1987). "Brain lectin-mediated agglutinability of dissociated cells from embryonic and postnatal mouse brain." Brain Res. 433: 146-150.
Kao, G. D., W. G. McKenna, et al. (1999). "p34 cdc2 kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells." J. Biol. Chem. 274: 34779-34784.
Kayyali, U. S., C. M. Pennella, et al. (2002). "Cytoskeletal changes in hypoxic pulmonary endothelial cells are dependent on MAPK-activated protein kinase MK2." J. Biol. Chem. 277: 42596-602.
Klemm, A. R., D. Young, et al. (1998). "Effects of polyethyleneimine on endocytosis and lysosome stability." Biochem. Pharmacol. 56: 41-6.
Kopitz, J., C. von Reitzenstein, et al. (2001). "Negative regulation of neuroblastoma cell growth by carbohydrate-dependent surface binding of galectin-1 and functional divergence from galectin-3." J. Biol. Chem. 276: 35917-23.
La, M., T. V. Cao, et al. (2003). "A novel biological activity for galectin-1: inhibition of leukocyte-endothelial cell interactions in experimental inflammation." Am. J. Pathol. 163: 1505-15.
Langer, R. and J. P. Vacanti (1993). "Tissue engineering." Science 260: 920-6.
Lavoie, J. N., G. L'Allemain, et al. (1996). "Cyclin D1 expression is regulated positively by the p42/p44MAPK and negatively by the p38/HOGMAPK pathway." J. Biol. Chem. 271: 20608-16.
Levy, Y., R. Arbel-Goren, et al. (2001). "Galectin-8 functions as a matricellular modulator of cell adhesion." J. Biol. Chem. 276: 31285-95.
Levy, Y., D. Ronen, et al. (2003). "Sustained induction of ERK, protein kinase B, and p70 S6 kinase regulates cell spreading and formation of F-actin microspikes upon ligation of integrins by galectin-8, a mammalian lectin." J. Biol. Chem. 278: 14533-42.
Lewandrowski, K. U., S. P. Bondre, et al. (2002). "Healing of osteochondral osteotomies after fixation with a hydroxyapatite-buffered polylactide. A histomorphometric and radiographic study in rabbits." Biomed. Mater. Eng. 12: 259-70.
Lewis, T. S., P. S. Shapiro, et al. (1998). "Signal transduction through MAP kinase cascades." Adv. Cancer Res. 74: 49-139.
Ma, J. B., H. G. Wang, et al. (2001). "A preliminary in vitro study on the fabrication and tissue engineering applications of a novel chitosan bilayer material as a scaffold of human fetal dermal fibroblasts." Biomaterials 22: 331-6.
Madihally, S. V. and H. W. Matthew (1999). "Porous chitosan scaffolds for tissue engineering." Biomaterials 20: 1133-42.
Maeda, N., N. Kawada, et al. (2003). "Stimulation of proliferation of rat hepatic stellate cells by galectin-1 and galectin-3 through different intracellular signaling pathways." J. Biol. Chem. 278: 18938-44.
Mahanthappa, N. K., D. N. Cooper, et al. (1994). "Rat olfactory neurons can utilize the endogenous lectin, L-14, in a novel adhesion mechanism." Development 120: 1373-84.
Marcon, P., E. Marsich, et al. (2005). "The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan." Biomaterials 26: 4975-84.
Mi, F. L., S. S. Shyu, et al. (2001). "Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing." Biomaterials 22: 165-73.
Miyamoto, S., H. Teramoto, et al. (1996). "Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAPK kinase activation:Roles of integrin aggregation and occupancy of receptors." J. Biol. Chem. 135: 1633-1642.
Moiseeva, E. P., Q. Javed, et al. (2000). "Galectin 1 is involved in vascular smooth muscle cell proliferation." Cardiovasc. Res. 45: 493-502.
Morgan, D. O., R. P. Fisher, et al. (1998). "Control of eukaryotic cell cycle progression by phosphorylation of cyclin-dependent kinases." Cancer J. Sci. Am. 4 Suppl 1: S77-83.
Mori, T., M. Okumura, et al. (1997). "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro." Biomaterials 18: 947-51.
Muschel, R. J., H. B. Zhang, et al. (1991). "Cyclin B expression in HeLa cells during the G2 block induced by ionizing radiation." Cancer Res. 51: 5113-7.
Noda, C., T. Masuda, et al. (2003). "Vanadate improves cardiac function and myocardial energy metabolism in diabetic rat hearts." Jpn. Heart J. 44: 745-757.
Otsuka, H., Y. Nagasaki, et al. (1999). "Reactive-PEG-polyatide block copolymer for tissue engineering." Polymer Preprints 2: 128-129.
Park, S. N., J. C. Park, et al. (2002). "Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking." Biomaterials 23: 1205-12.
Perillo, N. L., M. E. Marcus, et al. (1998). "Galectins:versatile modulators of cell adhesion, cell proliferation, and cell death." J. Mol. Med. 6: 402-412.
Poolman, R. A. and G. Brooks (1998). "Expressions and activities of cell cycle regulatory molecules during the transition from myocyte hyperplasia to hypertrophy." J. Mol. Cell. Cardiol. 30: 2121-35.
Renshaw, M. W., X. D. Ren, et al. (1997). "Growth factor activation of MAP kinase requires cell adhesion." EMBO J. 16: 5592-5599.
Rini, J. M. and Y. D. Lobsanov (1999). "New animal lectin structures." Current Opinion in Structure Biology 9: 578-84.
Rossomando, A. J., D. M. Payne, et al. (1989). "Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase." Proc. Natl. Acad. Sci. USA 86: 6940-3.
Sanford, G. L. and S. Harris-Hooker (1990). "Stimulation of vascular cell proliferation by beta-galactoside specific lectins." Faseb J. 4: 2912-8.
Sharma, C., T. Vomastek, et al. (2005). "MEK partner 1 (MP1): regulation of oligomerization in MAP kinase signaling." J. Cell. Biochem. 94: 708-19.
Shaul, Y. D. and R. Seger (2004). "Use of inhibitors in the study of MAPK signaling." Methods Mol. Biol. 250: 113-26.
Skrincosky, D. M., H. J. Allen, et al. (1993). "Galaptin-mediated adhesion of human ovarian carcinoma A121 cells and detection of cellular galaptin-binding glycoproteins." Cancer Res. 53: 2667-75.
Spira, M., J. Fissette, et al. (1969). "Evaluation of synthetic fabrics as artificial skin grafts to experimental burn wounds." J. Biomed. Mater. Res. 3: 213-34.
Suh, J. and H. Matthew (2000). "Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering:a review." Biomaterials 21: 1589-98.
Suzuki, S., K. Matsuda, et al. (1990). "Experimental study of a newly developed bilayer artificial skin." Biomaterials 11: 356-60.
Taravel, M. N. and A. Domard (1995). "Collagen and its interaction with chitosan. II. Influence of the physicochemical characteristics of collagen." Biomaterials 16: 865-71.
Tonks, N. K. (1996). "Protein tyrosine phosphatases and the control of cellular signaling responses." Adv. Pharmacol. 36: 91-119.
Tonks, N. K. and B. G. Neel (2001). "Combinatorial control of the specificity of protein tyrosine phosphatases." Current Opinion in Cell Biology 13: 182-95.
Ullrich, A. and J. Schlessinger (1990). "Signal transduction by receptors with tyrosine kinase activity." Cell 61: 203-12.
Varki, A. (1999). "Disappearing physician-scientists." Science 283: 791-2.
Walker, J. L., A. K. Fournier, et al. (2005). "Regulation of growth factor signaling and cell cycle progression by cell adhesion and adhesion-dependent changes in cellular tension." Cytokine Growth Factor Rev. Epub ahead of print.
Waskiewicz, A. J. and J. A. Cooper (1995). "Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeast." Current Opinion in Cell Biology 7: 798-805.
Wells, V. and L. Mallucci (1991). "Identification of an autocrine negative growth factor: mouse b-galactoside-binding protein is a cytostatic factor and cell groeth regulator." Cell 64: 91-97.
Wright, J. H., E. Munar, et al. (1999). "Mitogen-activated protein kinase kinase activity is required for the G(2)/M transition of the cell cycle in mammalian fibroblasts." Proc. Natl. Acad. Sci. USA 96: 11335-40.
Yamaguchi, M. and Y. Daimon (2005). "Overexpression of regucalcin suppresses cell proliferation in cloned rat hepatoma H4-II-E cells: Involvement of intracellular signaling factors and cell cycle-related ge." J. Biol. Chem. Epub ahead of print.
Yamanaka, K., K. Mishima, et al. (2000). "Expression of galectin-1 mRNA correlates with the malignant potential of Human Gliomas and expression of antisense galectin-1 inhibits the growth of 9 Glioma cells." J. Neurosci. Res. 59: 722-730.
Yamaoka, K., S. Ohno, et al. (1991). "Overexpression of a beta-galactoside binding protein causes transformation of BALB3T3 fibroblast cells." Biochem. Biophys. Res. Commun. 179: 272-9.
Zhou, Q. and R. D. Cummings (1993). "L-14 lectin recognition of laminin and its promotion of in vitro cell adhesion." Arch. Biochem. Biophys. 300: 6-17.
Zhu, P., Y. Masuda, et al. (2004). "The effect of surface charge on hydroxyapatite nucleation." Biomaterials 25: 3915-21.
Zhu, X., K. S. Chian, et al. (2004). "Effect of argon-plasma treatment on proliferation of human-skin-derived fibroblast on chitosan membrane in vitro." J. Biomed. Mater. Res. 73A: 264-274.

指導教授

黃榮南(Rong-Nan Huang)

審核日期

2005-7-23

推文