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姓名	翁棋蓉(Chi-Jong Weng)  查詢紙本館藏	畢業系所	物理學系
論文名稱	運用氘核磁共振研究POPE/Ergosterol膜之物理性質 (The study of the physical properties in POPE/Ergosterol model membranes using 2H NMR)
相關論文	★ 用氘核磁共振儀研究含高濃度麥角脂醇的DPPC人造膜之分子交交互作用 ★ Fluorescence study of lipid membranes containing sterol ★ 含固醇的脂質雙層膜的形態及相行為的研究 ★ The effects of composition and thermal history on the properties of supported lipid bilayers ★ The effect of sterol on the POPE/DPPC membranes ★ 麥角固醇對含膽固醇的脂雙層膜的影響 ★ Deuterium NMR Study of the Effect of Stigmasterol on POPE Membranes ★ Deuterium NMR Study of the effect of 7- dehydrocholesterol on the POPE Membranes ★ 運用氘核磁共振儀研究POPC/cholesterol膜之物理性質 ★ 模型細胞膜(含有相同碳鏈的PC/PE)存在或缺乏固醇類的物理性質 ★ 運用氘核磁共振研究DPPC/POPE/sterol人造細胞膜之物理性質 ★ Phase Behavior and Molecular Interactions of Membranes Containing Phosphatidylcholines and Sterol: A Deuterium NMR Study ★ The physical properties of phytosterol-containing lipid bilayers ★ An AFM Study on Supported Lipid Bilayers with and without Sterol ★ β-谷固醇對POPE膜物理特性的影響 ★ 固醇結構對PC膜物理特性的影響
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摘要(中)	近期的研究證據顯示，在生物膜中存在橫向異質的特性（lateral heterogeneities），這些異質的特性是指膜中有各種的脂質區塊(domain)，這些區塊中的脂質與蛋白質的組成成分及相關的物理特性都不同。這些脂質區塊，稱之為脂質浮排（lipid rafts），有高含量的膽固醇及抱合脂質(sphingolipids)且在許多生物過程中扮演重要的角色，不過，塊區的生成及相關的物理特性仍不太清楚。在含膽固醇的人造細胞膜中已被證實有液態有序(lo)及液態無序(ld)兩種液晶態區塊的存在，不只是膽固醇，其他的固醇也會使膜產生lo態。 我們運用氘核磁共振(2H NMR)光譜技術研究含有POPE和ergosterol兩種成分的人造細胞膜的相行為，我們藉核磁共振的光譜探測POPE膜，隨著ergosterol含量與溫度的改變，POPE膜中相的變化。研究發現，加入愈多的ergosterol，會使得膠態的POPE膜變的較無序；而會使得液晶態的POPE膜變的稍微有序。我們從核磁共振的光譜中可以得到POPE/ergosterol膜的部份相圖，當溫度小於Tm時，相圖顯示，有gel + lo 兩相共存的區域存在；當溫度大於Tm時，相圖顯示，POPE/ergosterol膜中只有一種液晶態存在。
摘要(英)	Recent evidence suggests that lateral heterogeneities exist in biological membranes of living cell. The heterogeneity is proposed to be a coexistence of lipid domains with differing degrees of order, lipid/protein compositions, and physical properties in the membrane. Cholesterol and sphingolipid-enriched domains (lipid rafts) have been implicated to play important roles in a wide range of biological processes. However, their existence and physical properties have been controversial. Model membranes containing cholesterol show that two liquid crystalline phases: liquid-ordered (lo) and liquid-disordered (ld) phases coexist in some membranes. Not only cholesterol promote the formation of the lo phase, other sterols such as ergosterol also promote the formation of the lo phase. We investigate the phase behavior of POPE/ergosterol binary model membranes using solid-state deuterium nuclear magnetic resonance (2H NMR). The sn-1 chain of POPE was perdeuterated and NMR spectra were taken as a function of temperature and ergosterol concentration. It is found that adding ergosterol disorders the gel-phase POPE membranes, while only slightly orders the liquid crystalline–phase membranes. The partial phase diagram of POPE/ergosterol, constructed from the NMR spectra, exhibits gel + lo phase coexistence region below Tm. Above Tm, the membranes display only one single liquid crystalline phase.
關鍵字(中)	★ 麥角固醇 ★ 氘核磁共振	關鍵字(英)	★ 2H NMR ★ POPE ★ ergosterol
論文目次	Abstract I 中文提要 II 致謝 III 目錄 IV 圖目錄 VII 表目錄 IX 索引表 X 前言 1 第一章 緒論 1-1 細胞膜的功能 1 1-2 流體鑲嵌模型（Fluid mosaic model） 2 1-3 脂質浮排(Lipid rafts) 3 1-4 脂質分子 5 1-5 膜的相行為（phase behavior） 6 1-6 人造生物膜（model membranes） 9 1-7 POPE與ergosterol 10 第二章 實驗方法 2-1 2H NMR基本原理 13 第三章 實驗流程 3-1 樣品配製 20 3-2 實驗藥品 22 3-3 2H NMR實驗參數設定 23 第四章 結果分析 24 結論 43 參考文獻 45
參考文獻	[1] Molecular Cell Biology, Fifth Edition, Matthew P Scott, et al [2] S. J. Singer and G. L. Nicolson, (1972). Science 175, 720-731 [3] J. F. Danielli and H. Davson, J. Cell Comp. Physiol. 5, 495-508 (1935). [4] K. Simons and E. Ikonen. (1997). Nature 387, 569–572 [5] D. A. Brown and E. London. (1998) J. Membr. Biol. 164, 103–114. [6] D. A. Brown and E. London. (2000) J. Biol. Chem. 275, 17221–17224. [7] J. M. Crane and L. K. Tamm（2004）Biophysical J. 86 2965–297 [8] E.Kuebler, H. G. Dohlman, and M. P. Lisanti. 1996.J. Biol. Chem. 271:32975-32980. [9] P.J. Casey (1995) Protein lipidation in cell signaling. Science 268, 221–225 [10] K. Simons and D. Toomre (2000) Nat. Rev. Mol. Cell Biol. 1, 31–41 [11] E. J. Smart, G. A. Graf, M. A. Mcniven, W. C. Sessa, J. A. Engelman, P. E. Scherer, T. Okamoto and M. P. Lisanti Mol. Cell Biol. 19, 7289–7304 [12] K. Simons and E. Ikonen. (2000).Cell. Biol. 1, 31–41. [13] K. Simons and E. Ikonen. (2000). Science 290, 1721–1726. [14] Michael E. (2003) Annu Rev. Biophys. Biomol. 32, 257-83 [15] C.Chachaty, D. Rainteau, C. Tessier, P.J. Quinn, and C. Wolf,（2005） Biophys J BioFAST, published on March 11 [16]E. Endress., S. Bayerl., K. Prechtel., C. Maier, R M. and T. M. B Bayerl. (2002) Langmuir, 18, 3293-3299 [17] Y. W. Hsueh, K. Gilbert, C. Trandum, M. Zuckermann, and J. Thewalt (2005) Biophys. J..2005; 88: 1799-1808. [18]S. L. Veatch, I. V. Polozov,y K. Gawrisch,y and S. L. Keller.(2004) Biophysical Journal Volume 86 May 2004 2910–2922 [19] M. R. Vist and J. H. Davis (1990) Biochemistry , 29, 45 1-464 [20] Philip L. Yeagle. (2004) The structure of biological membranes, 2nd edition CRC Press LLC, Boca Raton, p. 58. [21] Robert B Gennis. (1989) Biomembranes—molecular structure and function. Springer– Verlag, New York Inc. p. 40. [22] S. Tristram-Nagle, Y. Liu, J. Legleiter and J. F. Nagle (2002) Biophysical J.83 3324–3335 [23] M. Rappolt, P. Laggner and Georg Pabst (2004) Recent Res. Devel. Biophys., 3(2004): ISBN: 81-7895-130-4 [24] S. R. Shaikh, M. R. Brzustowizice, N. Gustafson, W. Stillwell and S. R. Wassall. (2002) Biochem J, 41, 10593-10602 [25] J. Loffler, H. Einsele, H. Hebart, U. Schumacher, C. Hrastink and G. Daum (2000). FEMS Microbiology Letters, 185, 59-63 [26] Philip L. Yeagle. (2004) The structure of biological membranes, 2nd edition CRC Press LLC, Boca Raton, p. 26. [27] Robert B Gennis. (1989) Biomembranes—molecular structure and function Springer– Verlag, New York Inc. p. 23. [28] K. Balasubramanian and C.M. Gupta European Journal of Biochemistry, 240, 798-806 [29] Boni L.T, Hui S.W (1983). Biochim Biophys Acta. 731(2):177-85 [30] S. R. Shaikh, M. R. Brzustowicz, W. Stillwell and S. R. Wassall (2001). Biochemical and Biophysical Research Communications 286, 758–763 [31] A. Arora, H. Raghuraman and A. Chattopadhyay (2004). Biochem. and Bio. J. Communication, 318, 920-926 [32] M. Schnabl, G. Daum and H. Pichler (2005). Biochem. Et Biophysica Acta, 1687, 130-140 [33] David L. Nelson, et al. (2004) Lehninger Principles of Biochemistry, 4th edition W. H. Freeman, New York, p. 361 [34] C.P.Slichter. (1990) Principles of magnetic resonance, Third editon, Springer – Verlag, New York, p. 485. [35] J.Seelig (1977), Q. Rev. Biophys.353-418 [36] Chantal Pare´ and Michel Lafleur (1998). Biophysical Journal Volume 74 899–909 [37] M. Vist, and J. H. Davis. (1990).Biochemistry. 29:451-464. [38] Y. W. Hsueh, K. Gilbert, C. Trandum, M. Zuckermann, and J. Thewalt (2005) Biophys. J.2005; 88: 1799-1808.
指導教授	薛雅薇(Ya-Wei Hsueh)	審核日期	2005-7-14
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