| 摘要(英) |
Ubiquitin is a common small protein in cells, it usually served as tag conjugated to the substrate, for DNA repair, proteolysis, and others. This process is called ubiquitination, which ubiquitinates specific substrates by ubiquitin activation enzyme, ubiquitin conjugating enzyme and ubiquitin ligase. Ubiquitin ligase serves as a critical point for substrates specificity. Pre- mRNA-processing factor 19 (PRP19) is one of the ubiquitin ligases, involves in DNA repair, mRNA splicing, and has been found overexpressed in lung cancer. I found forty-five substrates of PRP19 from in vitro ubiquitination assay on yeast proteome chip which contains more than 5800 kinds of the yeast protein. I used bioinformatics analysis to find PRP19 further involving ten classes of biological processes, seven classes of cellular components, six classes of molecule functions, and twelve classes of pathways by ubiquitinates its substrates. Forty of forty-five substrates involved in seven classes of biological processes, four classes of cellular components and two pathways which relate to PRP19.In addition, there were three classes of biological processes, six classes of cellular components, three classes of molecule functions were significantly enriched. Also, I found two groups of substrates had different consensus motifs as putative binding sites with PRP19. According to the result, I found those GO and pathways might be regulated by PRP19-substrates ubiquitination, it gave the direction for
further study on PRP19. |
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