基因體的普適性之成長模型

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姓名

范文郎(Wen-lang Fan) 查詢紙本館藏

畢業系所

物理學系

論文名稱

基因體的普適性之成長模型
(Modeling Genome Growth Based on Universal Properties of Whole Genome)

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摘要(中)

片段複製被認為是生物基因體演化與成長的最主要驅動力，但對於生物複雜性與基因體長度的明確關係卻尚未明瞭。利用寡核苷酸頻率法分析了865條完整的染色體後，歸納出基因體全序列具有等價長度、倒對稱與長程關聯等普適特徵。藉由這些普適的統計性質，我們提出以片段複製為基礎的成長機制，並建立模型來重現了生物基因體的普適特徵。模型由隨機片段複製後，以隨機插入、倒字串插入或連接插入等方式來成長，並加入單點突變來架構整個模型的成長機制。在要求吻合基因體普適性來探索模型參數值的過程中，我們發現模型序列的特性與複製片段的長度沒有明顯關聯，而對單點突變有較強的依靠性。在選擇適當的參數下，模型序列具有與基因體全序列相同的統計性質，驗證了模型成長機制可能就是生物基因體成長的主要生化機能。

摘要(英)

Segmental duplication has long been considered to be an important driving force in genome growth and evolution. But a quantitative description of the nature of the duplication process and its relation to the complexity of genome structure has been lacking. We use word frequency to analyze complete genomes and use non-trivial universal statistical properties of genomes – equivalent length, inverse symmetry and long-range variation – as clues for specifying the nature of the segmental duplication process. We use a minimal genome growth model based on random segmental duplication (RSD) to generate genome-length sequences and compare their statistical properties with those of real genomes. With a few biologically meaningful universal parameters the RSD model can well describe most of the prominent and non-trivial statistical properties of genomes, including the universality of their equivalent lengths, and their patterns of long-range variation and inverse symmetry. Neutral and mostly random segmental duplication (RSD) is a dominant characteristic of genome growth, with the typical length of duplicated segments (DS) being 500 to 5000 nucleotides long. About 70% of the duplication events are “tandem” – DS is proximal to its origin – and about 30% are inverse – DS is made from one strand to the other. Occasionally a whole genome is inversely duplicated.

關鍵字(中)

★ 片段複製
★ 基因體成長模型
★ 長程關聯
★ 倒對稱
★ 等價長度

關鍵字(英)

★ long-range variation
★ inverse symmetry
★ Segmental duplication
★ genome growth model
★ equivalent length

論文目次

目錄
摘要 I
Abstract II
誌謝 III
目錄 IV
圖目錄 VI
表目錄 VIII
第一章緒論 1
1.1 研究背景 1
1.2 基因體的特徵 4
1.2.1 DNA的結構 4
1.2.2 DNA中的重複序列 6
1.2.3 突變、修復與重構 7
1.3 基因體的進化模式 8
1.3.1 最初的基因體 9
1.3.2 新基因的產生 9
第二章基因體的統計性質與成長模型 13
2.1 實驗材料 13
2.2 基因體的統計性質與模型機制 14
2.2.1 字串間隔分佈 14
2.2.2 頻譜分佈與m-sets 16
2.2.3 等價長度 17
2.2.4 序指數 23
2.2.5 倒對稱性 25
2.2.6 基因體中的長程關聯性 28
第三章基因體成長模型的結果 36
3.1 最簡約隨機片段複製模型 36
3.1.1 插入片段的長度與點突變的比率 36
3.1.2 隨機片段複製 (RSD)模型的特性 38
3.2 倒片段複製 39
3.2.1 倒片段複製成長對等價長度的影響 39
3.2.2 參數（μ, i）的最佳解空間 39
3.3 接連複製片段的比例與程度對長程關聯的效應 40
3.3.1 以接連片段複製造成GC含量的長程關聯 40
3.3.2 模型同時滿足等價長度、倒對稱性與長程關聯 41
第四章討論、結論與未來展望 43
4.1 基因體的生長機制 43
4.2 GC含量的長程變化 44
4.3 生命保護機制 44
4.4 結論與未來展望 45
　　　　　參考文獻　　 47

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指導教授

李弘謙(Hoong-chien Lee)

審核日期

2009-7-26

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