使用微型閥並具備自動平台校正功能之三維生物列印機開發

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洪承暉(CHENG-HUI HUNG) 查詢紙本館藏

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機械工程學系

論文名稱

使用微型閥並具備自動平台校正功能之三維生物列印機開發
(Development of Microvalve-based Three-demensional Bioprinter with Automatic Levelling Platform)

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摘要(中)

三維生物列印是結合了組織工程與積層製造技術的優勢，來製作生物支架或人工組織器官的製程，解決人體組織或器官移植和修復等問題。其中由於積層製造技術的引入，使製作多孔性且複雜外型輪廓的生物支架更有效率。為了提升工作平台水平度與縮短校正水平時間，本研究將開發一台具有自動水平校正功能的生物列印機，並且採用微型閥噴墨的方式列印材料。擁有快速沉積以及較低的預壓力以防止細胞損傷，並且提升溫度控制能力，提供更好的列印環境來製作生物支架。
本研究所發展之生物列印機系統包括運動控制模組、溫度循環控制模組、微型閥噴墨模組與距離感測模組。其中新設計開發的自動水平校正平台分類在運動控制模組中，擁有三軸之線性馬達作為同步驅動，主要功能除了使用自動校正水平取代人工調校之外，也包含了控制工作平台的升降，並且開發C#環境之人機介面方便人員操作。
由實際使用微型閥噴墨方式列印結果得知該方法為可行，且擁有非接觸式列印的優點，無須考量針頭與列印支架之間的碰撞與材料沾黏的問題。透過列印參數的優化可以提升列印品質，並且針對不同參數所列印之結果做出綜合比較，最後列印高層數支架。

摘要(英)

Three-dimensional bioprinting combines the advantages of tissue engineering and additive manufacturing technology to make biological scaffolds or artificial tissue and organs, solving problems such as human tissue or the transplantation and repairing of organ. Among them, due to the introduction of additive manufacturing technology, it is more efficient to make porous and complex contoured biological scaffolds. In order to improve the level of the working platform and shorten the levelling time, this study is going to develop a bio-printer with automatic levelling and the material printed by micro-valve inkjet. In order to prevent cell damage, it possesses rapid deposition and low pre-pressure. In addition, it enhances temperature control ability, provides a better printing environment to manufacture biological scaffolds.
The bio-printer system developed by this study includes motion control module, temperature cycling control module, micro valve inkjet module and distance sensing module. The newly designed automatic leveling platform is classified into motion control module, and has a three-axis linear motor as synchronous drive. In addition to the main function is using automatic leveling as the replacement of manual adjustment, it also contains the control of working platform lifting, and develop the user interface of C# environment to let the operation convenient for user.
It is found that the method is feasible by the actual use of microvalve inkjet printing results, and has the advantages of non-contact printing, no need to consider the collision between needle and scaffold, as well as the problem of material adhesion. The printing quality can be improved by optimizing the printing parameters; furthermore, for comprehensive comparison of the printed results by different parameters is made, and print the high-level scaffold ultimately.

關鍵字(中)

★ 組織工程
★ 組織工程支架
★ 3D生物列印機
★ 微型閥噴墨
★ 自動水平校正

關鍵字(英)

★ Tissue engineering
★ Tissue engineering scaffold
★ 3D Bioprinter
★ Microvalve inkjet
★ Automatic levelling

論文目次

摘要 I
ABSTRACT II
誌謝 III
目錄 IV
圖目錄 VI
表目錄 X
第一章緒論 1
1-1前言 1
1-2文獻回顧 2
1-3研究動機與目的 15
1-4論文架構 16
第二章研究與理論說明 17
2-1組織工程簡介 17
2-2組織工程用積層製造技術簡介 19
2-3 平台之水平校正演算法 27
2-4組織工程支架材料 30
2-5舊型低溫積層製造系統簡介 31
第三章系統架構與實驗方法 33
3-1三維生物列印機之各模組簡介 33
3-2 自動水平校正平台機構設計介紹 39
3-3 軟體與硬體設備整合 42
3-4人機介面與自動化水平校正流程 47
3-5列印參數設計 58
3-6使用之材料介紹 60
第四章實驗結果與討論 61
4-1工作平台水平度分析與比較 61
4-2工作平台溫度分佈分析 66
4-3使用不同參數列印之實驗分析 71
4-4使用最佳參數之支架製作 77
第五章結論與未來展望 81
5-1結論 81
5-2未來展望 82
參考文獻 83

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指導教授

廖昭仰(CHAO-YAUG LIAO)

審核日期

2018-10-12

推文