薑黃素微脂體的製備與安定性之研究

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姓名

李懿芸(Yi-Yun Lee) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

薑黃素微脂體的製備與安定性之研究
(Preparation of Curcuminoid-loaded Liposomes)

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摘要(中)

本研究的目的是透過薄膜水合法製備類薑黃素微脂體之溶液，將類薑黃素包覆於微脂體中，避免類薑黃素被溫度和光線分解，並且改善類薑黃素的水溶性，進而提升生物利用度。本研究以乳化劑如tween 80、tween 20、tween 80/span 80、和 tween 80/span 85，以及磷脂質和膽固醇，作為微脂體的包覆材料。此外，以乙醇而非傳統方法中以氯仿做為溶劑，形成球狀的雙分子磷脂層膜。本研究改變了不同比例及種類的乳化劑來製備微脂體溶液，並將樣品放置於 4 ℃ 的環境下儲存，對其做安定性的測試。此外，挑選包覆率佳的樣品分別放置在室溫及40 ℃ 的環境下進行安定性的測試。最後，挑選包覆率最佳的樣品進行放大測試，檢驗類薑黃素微脂體之溶液商業化的潛力。本研究中利用紫外光-可見光-近紅外光分光光譜儀測量類薑黃素微脂體之溶液濃度，並計算其包覆率及類薑黃素總濃度。此外，利用動態光散射分析儀做粒徑測量，並以光學顯微鏡及穿透式電子顯微鏡對其外觀進行觀測。最後找出最佳的合成比例為Phosphatidylcholine：Cholesterol：Tween 80 = 4:1:50 (reagent grade)，且樣品存放一百天後仍然穩定。

摘要(英)

The purpose of this study was to prepare a curcuminoid-loaded liposome solution through the thin film hydration method to encapsulate curcuminoid in the liposomes to prevent curcuminoid from being decomposed at high temperature and light, and to improve the solubility of curcuminoid in water, thereby enhancing the bioavailability. In this study, emulsifiers such as tween 80, tween 20, tween 80/span80, and tween80/span 85, as well as phosphatidylcholine and cholesterol, were used as liposome coating materials. Furthermore, ethanol was used as a solvent, instead of chloroform in the traditional method, to form a spherical bimolecular phospholipid layer membrane. In this study, different ratios and types of emulsifiers were used to prepare liposome solutions, and the samples were stored at 4℃ to test their stability. Furthermore, select samples with good encapsulation efficiency and place them at room temperature and 40℃ for stability testing. Finally, the samples with the best encapsulation efficiency were selected for scale-up testing to test the commercialization potential of curcuminoid-loaded liposome solution. UV-visible-near-infrared spectroscopy was used to measure the concentration of curcuminoid liposomes, and to determine the encapsulation efficiency and total curcuminoid concentration. The dynamic light scattering was used for particle size measurement, and its appearance was observed with an optical microscope and transmission electron microscopy. The best preparation condition was phosphatidylcholine: cholesterol: tween 80 = 4: 1: 50, and the sample was very stable after 100 days of storage.

關鍵字(中)

★ 類薑黃素
★ 微脂體
★ 包覆材料
★ 生物材料
★ 生醫材料
★ 營養補充物

關鍵字(英)

★ Curcuminoids
★ liposome
★ encapsulation
★ biomaterial

論文目次

中文摘要 i
Abstract ii
Acknowledgements iii
Table of Contents iv
List of Tables vi
List of Figures viii
CHAPTER 1 INTRODUCTION 1
CHAPTER 2 LITERATURE REVIEW 1
2.1 Curcuma longa 1
2.1.1 Distribution of Curcuma longa 1
2.1.2 History of Curcuma longa 2
2.2 Curcuminoid 3
2.2.2 Characteristics of curcuminoids 4
2.2.3 Safety of curcumin 7
2.2.4 Application of curcumin 8
2.3 Liposome technology 12
2.3.1 Composition of liposome 12
2.3.2 Methods of liposome preparation 13
2.4 Hydrophile Lipophilic Balance 17
CHAPTER 3 EXPERIMENTAL 21
3.1 Materials 21
3.2 Preparation of curcuminoids-loaded liposome solution 21
3.3 Homogenizer 23
3.4 Determination of total curcuminoids in liposome 24
3.3.1 Optical microscopy (OM) 24
3.3.2 Transmission electron microscopy (TEM) 25
3.3.3 Ultraviolet-Visible-near-IR Spectroscopy (UV-Vis-NIR) 26
3.3.4 Dynamic Light Scattering (DLS) 27
CHAPTER 4 RESULTS AND DISCUSSION 29
4.1 Introduction 29
4.2 Effects of surfactant on the encapsulation efficiency 30
4.2.1 Reagent grade polysorbate 80 31
4.2.2 Technical grade polysorbate 80 40
4.2.3 Technical grade polysorbate 20 46
4.2.4 Technical grade polysorbate 80/ Sorbitan Monooleate 51
4.2.5 Technical grade polysorbate 80/ Sorbitan Trioleate 57
4.2.6 Conclusion 63
4.3 Effects of storage temperature 71
4.3.1 Encapsulation efficiency 71
4.3.2 particle size 77
4.3.3 Appearance study 78
4.4 Scale up test 81
4.4.1 Encapsulation efficiency 82
4.4.2 particle size 85
4.4.3 Appearance study 86
CHAPTER 5 CONCLUSIONS 88
REFERENCES 90

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指導教授

陳郁文(Yu-Wen Chen)

審核日期

2021-7-2

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