| 摘要(英) |
Domestic wastewater contains ammonia nitrogen substances and organic acids, among which ammonia water, propionic acid and butyric acid often cause pungent odors. However, the method and process of degrading ammonia nitrogen substances and organic acids are cumbersome, and if they are not properly treated, and straightly discharged into the sewer, which will have a negative impact on the environment.
Bacillus subtilis is a Gram-positive bacterium that belongs to the Bacillus family. It is a bacterium that widely exists in the natural environment and is highly heat-resistant and capable of forming spores to adapt to harsher environments. Bacillus subtilis has a variety of metabolic pathways, which can metabolize pollutants such as organic matter and nitrogen sources, and convert them into harmless products.
Therefore, in this experiment Bacillus subtilis BCRC10029 is used as a method to treat domestic wastewater. By adding 10% (v/v) of the bacterial suspensions to a concentration of 0.1M ammonia, propionic acid, butyric acid and the mixed solution of the above three, a sample is taken every 24 hours for a total of 7 days, and the change of ammonia nitrogen content with the number of days was detected with a spectrophotometer, and the content of propionic acid and butyric acid with the number of days was detected with a gas chromatograph.
The experimental results show that no matter the content of ammonia nitrogen or the content of propionic acid and butyric acid, after fermentation with Bacillus subtilis BCRC10029, the contents of the three all have a downward trend with the increase of days. Under the best environmental conditions, Bacillus subtilis BCRC10029 The ammonia nitrogen consumed by the degradation of Bacillus subtilis BCRC10029 decreased by 84.4%, propionic acid was degraded and consumed from 3.54% to 0%, and butyric acid also decreased from 36.1% to 15.4%. In the mixed solution, the changes of ammonia nitrogen were 56.9%, 52.2%, 65.9% and 58.8% when inoculated with different bacterial concentration from low to high. The degradation and consumption of propionic acid by different concentrations of bacteria suspensions, the residual amount of propionic acid tended to 0 on the 7th day of fermentation, while the residual amount of butyric acid also decreased to a certain extent, showing that Bacillus subtilis BCRC10029 has the ability to degrade and consume ammonia nitrogen, propionic acid and butyric acid. |
| 參考文獻 |
1. Thomas, O. and M.-F. Thomas, Chapter 12 - Industrial wastewater, in UV-Visible Spectrophotometry of Waters and Soils (Third Edition), O. Thomas and C. Burgess, Editors. 2022, Elsevier. p. 385-416.
2. G, L.
3. Chiu, Y.-C., et al., Control of carbon and ammonium ratio for simultaneous nitrification and denitrification in a sequencing batch bioreactor. International biodeterioration & biodegradation, 2007. 59(1): p. 1-7.
4. Khardenavis, A.A., A. Kapley, and H.J. Purohit, Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Applied Microbiology and Biotechnology, 2007. 77: p. 403-409.
5. Khin, T. and A.P. Annachhatre, Novel microbial nitrogen removal processes. Biotechnology advances, 2004. 22(7): p. 519-532.
6. Yang, X.-P., et al., Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying–denitrifying bacterium, Bacillus subtilis A1. Bioresource technology, 2011. 102(2): p. 854-862.
7. Ilyas, M., et al., Environmental and health impacts of industrial wastewater effluents in Pakistan: a review. Rev Environ Health, 2019. 34(2): p. 171-186.
8. Buczyńska, A., R. Rolecki, and S. Tarkowski, [Industrial wastes and health hazards]. Med Pr, 1999. 50(2): p. 179-90.
9. Turnbull., P.C.B., Bacillus, in Medical Microbiology, S. Baron, Editor. 1996.
10. Britannica, E., bacillus. 7 May. 2020.
11. Errington, J. and L.T.V. Aart, Microbe Profile: Bacillus subtilis: model organism for cellular development, and industrial workhorse. Microbiology (Reading), 2020. 166(5): p. 425-427.
12. Paul, S.I., et al., Identification of marine sponge-associated bacteria of the Saint Martin′s island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita. Aquaculture, 2021. 545: p. 737156.
13. Rahman, M.M., et al., Whole-Genome Sequence of Bacillus subtilis WS1A, a Promising Fish Probiotic Strain Isolated from Marine Sponge of the Bay of Bengal. Microbiol Resour Announc, 2020. 9(39).
14. Serra, C.R., et al., Sporulation during growth in a gut isolate of Bacillus subtilis. Journal of bacteriology, 2014. 196(23): p. 4184-4196.
15. Hirooka, K., Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion. Bioscience, biotechnology, and Biochemistry, 2014. 78(9): p. 1471-1484.
16. Philippe Noirot, P.P.a.M.-F.N.-G., Bacillus: Cellular and Molecular Biology. Replication of the Bacillus subtilis Chromosome. May 2007: Caister Academic Press.
17. Earl, A.M., R. Losick, and R. Kolter, Ecology and genomics of Bacillus subtilis. Trends Microbiol, 2008. 16(6): p. 269-75.
18. Hu, P., et al., Sensing of nitrogen limitation by Bacillus subtilis: comparison to enteric bacteria. J Bacteriol, 1999. 181(16): p. 5042-50.
19. Gauvry, E., et al., Effects of temperature, pH and water activity on the growth and the sporulation abilities of Bacillus subtilis BSB1. International Journal of Food Microbiology, 2021. 337: p. 108915.
20. Sidorova, T.M., et al., Optimization of laboratory cultivation conditions for the synthesis of antifungal metabolites by bacillus subtilis strains. Saudi Journal of Biological Sciences, 2020. 27(7): p. 1879-1885.
21. Yang, T., et al., A Novel Regulator Participating in Nitrogen Removal Process of Bacillus subtilis JD-014. Int J Mol Sci, 2021. 22(12).
22. Schilling, O., et al., Transcriptional and metabolic responses of Bacillus subtilis to the availability of organic acids: transcription regulation is important but not sufficient to account for metabolic adaptation. Appl Environ Microbiol, 2007. 73(2): p. 499-507. |
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