台灣綠蜂膠對歐洲紫杉醇抗藥性攝護腺癌細胞的抑制效果探討

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姓名

阿斯拉(Asra) 查詢紙本館藏

畢業系所

生命科學系

論文名稱

台灣綠蜂膠對歐洲紫杉醇抗藥性攝護腺癌細胞的抑制效果探討
(Suppressive Effects of Taiwanese Green Propolis on Docetaxel Resistant Prostate Cancer Cells)

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至系統瀏覽論文 (2025-1-18以後開放)

摘要(中)

台灣綠蜂膠對歐洲紫杉醇抗藥性攝護腺癌細胞的抑制效果探討

中文摘要

歐洲紫杉醇於 2004 年被 FDA 批准為轉移去勢抗性攝護腺癌的治療藥物。然而，大多數這些患者的癌症在幾個月後對歐洲紫杉醇產生了抗藥性。台灣綠蜂膠以其抗炎、抗氧化和抗微生物特性而著稱，但台灣綠蜂膠對攝護腺癌的作用和機制尚不清楚。我們研究了台灣綠蜂膠對歐洲紫杉醇抗藥性攝護腺癌細胞 PC/DX25 細胞生長和存活的抑製效果。我們還探討了台灣綠蜂膠對PC/DX25細胞存活和生長重要信號蛋白的影響。我們的結果表明，台灣綠蜂膠可抑制歐洲紫杉抗藥性攝護腺癌細胞的生長與存活。

摘要(英)

Suppressive Effects of Taiwanese Green Propolis on Docetaxel Resistant Prostate Cancer Cells

Abstract

Docetaxel is used widely for the treatment of metastasis castration resistant prostate cancer (mCRPC). However, majority of these patient’s cancer became resistant to docetaxel after months. Taiwanese green propolis (TGP) is known for its anti-inflammation, anti-oxidation, and anti-microbial properties, but the effects of TGP on prostate cancer (PCa) are unclear. We investigated the suppressive effect of TGP on proliferation and morphology of docetaxel-resistant PCa cells PC/DX25. We examined the effects of TGP on signaling proteins involved in survival and proliferation of PCa cells. Our results suggested that TGP can suppress the proliferation and survival of docetaxel-resistant PCa.

關鍵字(中)

★ 0000000000000000000000

關鍵字(英)

★ TGP
★
★ Taiwanese green propolis

論文目次

Table of Contents
Chinese Abstract ……………………………………………………………………………………………………… I
English Abstract ……………………………………………………………………………………………………… II
Acknowledgments ……………………………………………………………………………………………………… III
Table of Contents ………………………………………………………………………………………………………. IV-V
List of Figures ………………………………………………………………………………………………………. VI
List of Tables …………………………………………………………………………………………………………. VI
Chapter I Introduction………………………………………………………………………………………. 1
1-1 Prostate……………………………………………………………………………………………. 1
1-2 Prostate Cancer ………………………………………………………………………………. 1-2
1-3 Propolis……………………………………………………………………………………………. 2-3
1-4 Taiwanese Green Propolis………………………………………………………………… 4
Chapter II Aim and flow chart of the study………………………………………………………… 5
2-1 The aim of the study…………………………………………………………………………. 5
2-2 Experimental flow chart…………………………………………………………………… 5
Chapter III Material and Method………………………………………………………………………. 6
3-1 Chemicals…………………………………………………………………………………………. 6
3-1.1 Antibodies…………………………………………………………………………………………. 6
3-1.2 Cell lines ……………………………………………………………………………............... 6
3-1.3 Cell subculture…………………………………………………………………………………… 6
3-1.4 Cell frozen…………………………………………………………………………………………. 7
3-1.5 Cell Thawing………………………………………………………………………………………. 7
3-2 Cell proliferation assay………………………………………………………………………. 7
3-3 Comet assay ……………………………………………………………………………………… 7
3-4 Immunofluorescence assay……………………………………………………………… 8
3-5 Western blotting analysis…………………………………………………………………. 8
3-5.1 Protein concentration measurement ……………………………………………. 8
3-5.2 SDS-page electrophoresis………………………………………………………......... 8
3-5.3 Transfer the gel……………………………………………………………………………… 9
3-6 Overexpression of Bcl-2 …………………………………………………………………… 9
3-7 Statistical analysis.…………………………………………………………………………… 10

Chapter 3 Material and Method
3-1 Chemicals………………………………………………………………………………………………………………………………….20
3-1.1 Antibodies …………………………………………………………………………………………………………………..20
3-2 Cell culture
3-2.1 Cell lines …………………………………………………………………………………………………………………….20
3-2.2 Cell subculture………………………………………………………………………………………………………………21
3-2.3 Cell frozen…………………………………………………………………………………………………………………….21
3-2.4 Cell Thawing…………………………………………………………………………………………………………………21
3-3 Cell proliferation assay………………………………………………………………………………………………………….21-22
3-4 Immunofluorescence assay……………………………………………………………………………………………………....22
3-5 Comet assay …………………………………………………………………………………………………………………………….22
3-6 Western blotting analysis…………………………………………………………………………………………………….23-24
3-6.1 Protein concentration measurement ………………………………………………………………………….23
3-6.2 SDS-page electrophoresis…………………………………………………………………………………………….23
3-6.3 Transfer the gel……………………………………………………………………………………………………………24
3-7 Overexpression of Bcl2 …………………………………………………………………………………………………………….24
3-8 Statistical analysis…………………………………………………………………………………………………………………….25

Chapter IV Results………………………………………………………………………………………… 10
4-1 Cell proliferation and the survival of PC-3 cells and docetaxel-resistant
PC/DX25 cells suppressed by the TGP treatment……………………………. 10
4-2 Apoptosis induced in PC/DX25 cells and PC-3 cells by the TGP
Treatment………………………………………………………………………………………. 10
4-3 TGP treatment effects the expression of signaling protein in PC/DX25
and PC-3 Cells………………………………………………………………………………… 10
4-4 Bcl-2 over-expression resistant to cell proliferation in docetaxel
Resistant prostate cancer cells…………………………………………………………………… 11
Chapter V Discussion …………………………………………………………………………………………. 11-12
Chapter VI Conclusion………………………………………………………………………………………… 12
6-1 Figures and Figures legends………………………………………………………. 13-19
6-2 List of antibodies…………………………………………………………………………… 20
6-3 Abbreviations……………………………………………………………………………. 21-22
6-4 References………………………………………………………………………………. 23-26

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指導教授

褚志斌(CHIH-PIN CHUU)

審核日期

2023-1-19

推文