通過多醣表面修飾增強隱形眼鏡的親水性和抗菌性能

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姓名

張晨渝(Chen-Yu Chang) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

通過多醣表面修飾增強隱形眼鏡的親水性和抗菌性能
(Enhancing the Hydrophilicity and Antibacterial Properties of Contact Lenses through Polysaccharide Surface Modification)

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摘要(中)

隨著隱形眼鏡的廣泛，由於其固有的潤濕性差而導致眼部併發症的相關風險構成了重大挑戰。隱形眼鏡每日貼附在角膜上，空氣中或雙手上的微生物均有機會因鏡片清潔不足而入侵眼睛，導致眼角膜發炎、疼痛等受損情況。因此，隱形眼鏡需具有抗菌性的功能，來維持眼睛的健康。眼睛乾澀是目前配戴隱形眼鏡最常面臨的問題，伴隨著鏡片上的沉澱物（淚液中的蛋白質和脂質），會造成視覺對比度的下降，使配戴不舒適而減少配戴時間。
為了解決這些問題，我們採用多醣體對隱形眼鏡進行表面改接觸繳過應用這些多醣，我們的目標是製造具有增強耐用性的多層抗菌膜，從而改善隱形眼鏡和眼表之間的相互作用。這種修改旨在引入更具生物相容性和對眼睛友好的親水層。我們的研究涉及一系列全面的評估，以評估多醣改質對隱形眼鏡的影響，特別注重其潤濕性、抗菌功效和抗蛋白質黏附能力。
綜合以上考量，隱形眼鏡不僅需要具備抗菌性以維持眼睛健康，還需具備抗蛋白沉積功能以增加配戴者的舒適度。我們的研究目的是藉由多醣體表面改質，提供更好的潤濕性、抗菌性和抗蛋白質黏附性，從而全面提升隱形眼鏡的性能和使用體驗。

摘要(英)

With the widespread adoption of contact lenses, the associated risks of ocular complications due to their inherent poor wettability present a significant challenge. Contact lenses, worn daily on the cornea, are susceptible to microbial invasion from the air or hands if not properly cleaned, leading to corneal inflammation, pain, and other damages. Therefore, contact lenses need antibacterial properties to maintain eye health. Dry eyes are the most common issue faced by contact lens wearers today, accompanied by deposits on the lenses (proteins and lipids from tears), which reduce visual contrast and cause discomfort, leading to shorter wear times.
To address these issues, we use polysaccharides to modify the surface of contact lenses. We aim to create durable, multilayer antibacterial films that enhance the interaction between the contact lens and the ocular surface by applying these polysaccharides. This modification is designed to introduce a more biocompatible and eye-friendly hydrophilic layer. Our research involves a comprehensive evaluation to assess the impact of polysaccharide modification on contact lenses, focusing on wettability, antibacterial efficacy, and resistance to protein adhesion.
In summary, contact lenses need antibacterial properties to maintain eye health and anti-protein deposition functionality to enhance wearer comfort. Our research aims to use polysaccharide surface modifications to provide better wettability, antibacterial properties, and resistance to protein adhesion, thereby comprehensively improving contact lenses′ performance and user experience.

關鍵字(中)

★ 隱形眼鏡
★ 表面修飾
★ 抗蛋白質吸附
★ 濕潤性
★ 親水性
★ 接觸角

關鍵字(英)

★ Contact Lens
★ Surface Modification
★ Anti-Protein Adhesion
★ Wettability
★ Hydrophilicity
★ Contact angle

論文目次

Abstract in Chinese i
Abstract ii
Acknowledgments iii
1. Introduction 1
1.1 Research Background 1
1.2 Research Purpose 2
1.3 Development History of Contact Lenses 3
1.4 Classification of contact lenses 4
1.5 Polymer Surface Modification 6
1.6 Manufacturing methods of contact lenses 7
1.7 Layer-by-layer assembly 9
1.8 About Chitosan (CHI) 10
1.9 About Carboxymethylcellulose (CMC) 11
1.10 About Branched polyethylenimine (BPEI) 13
1.10 About EDC/NHS 14
1.11 About Glutaraldehyde (GA) 16
2. Materials and Methods 18
2.1 Materials 18
2.1.1 Reagent and Brand 18
2.1.2 Equipment 18
2.2 Methods 19
2.2.1 Experimental Process 19
2.2.2 Experimental principle and methods 19
2.2.2.1 Experimental principle 19
2.2.2.2 Experimental Methods-modification 20
2.2.2.3 Experimental Methods- Contact angle 22
2.2.2.4 Experimental Methods- Spectral transmittance 24
2.2.2.5 Experimental Methods- Water content 25
2.2.2.6 Experimental Methods- Mechanical properties 26
2.2.2.7 Experimental Methods- Oxygen permeability 26
2.2.2.8 Experimental Methods- Thickness 30
2.2.2.9 Experimental Methods-Refractive index 31
2.2.2.10 Experimental Methods- Lens appearance and visual clarity 32
2.2.2.11 Experimental Methods-Protein Adsorption Assay 35
2.2.2.12 Experimental Methods- Antimicrobial Assay 36
2.2.2.13 Experimental Methods-Cytotoxicity test 37
3. Result 39
3.1 Contact angle 39
3.2 Spectral transmittance 45
3.3 Water content 46
3.4 Mechanical properties 47
3.5 Oxygen permeability 48
3.6 Refractive Index 52
3.7 Lens appearance and visual clarity 53
3.8 Thickness 57
3.9 Protein Adsorption Assay 57
3.10Antimicrobial Assay 59
3.11 Cytotoxicity test 60
4. Conclusion 63
5. Reference 66

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指導教授

黃貞翰(Chen-Han Huang)

審核日期

2024-8-21

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