光聲訊號對皮下血紅蛋白濃度定量之研究

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姓名

馮麒(Qi Feng) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

光聲訊號對皮下血紅蛋白濃度定量之研究
(Study on the quantification of subcutaneous hemoglobin concentration using photoacoustic signal)

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至系統瀏覽論文 (2029-7-23以後開放)

摘要(中)

本論文啟發於對瘀傷年齡判定的相關研究，隨時間瘀傷內部物質會產生濃度上的變化，因此定量物質濃度在預測瘀傷年齡的相關研究中佔有相當重要的一部分。本研究目的為開發一套光聲系統，利用其對目標吸收特徵的選定與擁有提供深度資訊的特性，能夠對目標物質的濃度進行定量。為了模擬人體皮膚組織，實驗中製作了以吉利丁為基底的仿體，內部埋入毛細管與打入紅色色素 Ponceau 4R，模擬血管與血液，並利用 532 nm雷射作為激發光源，對 Ponceau 4R 進行激發，透過調整仿體內部物質與仿體厚度，量測
不同情況下光聲訊號強度隨色素體積百分濃度變化的趨勢，討論所架設的光聲系統在實驗上的限制與問題。
除此之外，經由反覆量測特定濃度範圍之色素，對訊號強度的變化進行線性擬合，並將擬合線段作為濃度定量之模型，隨後對隨機已知濃度作光聲訊號強度的測定，將結果代入模型中，得到濃度預測值，將其與實際濃度值進行比較並討論，為此架構在定量的功能上建立基礎，最後針對研究過程中遇到的問題制定系統未來開發上的目標。

摘要(英)

This paper is inspired by research on the determination of the age of bruises. Over time, the concentration of substances inside bruises changes, making quantitative substance concentration an important part of predicting bruise age. The purpose of this study is to develop
a photoacoustic system, by selecting and possessing the absorption characteristics of the target, provides depth information and can quantify the concentration of the target substance.
To simulate human skin tissue, the experiment created gelatin-based phantoms embedded with capillaries tube and injected with red dye Ponceau 4R to simulate blood vessels and blood, respectively. A 532 nm laser was used as the excitation light source to excite the Ponceau 4R. By adjusting the substance inside the phantom and its thickness, the study measured the trend of photoacoustic signal intensity with changes in the volume percentage concentration of the dye under different conditions, discussing the limitations and issues of the constructed photoacoustic system in the experiment.
Furthermore, by repeatedly measuring the signal intensity variation of specific concentration ranges of the dye and performing linear fitting, a concentration quantification
model was established. Subsequently, the signal intensity of known random concentrations was measured using photoacoustics, and the results were plugged into the model to obtain concentration predictions. These predictions were compared with actual concentration values and discussed to establish the foundation for the quantitative function of this framework. Finally, goals for future development of the system were formulated based on the problems encountered during the research process.

關鍵字(中)

★ 光聲訊號
★ 血紅蛋白
★ 濃度

關鍵字(英)

★ photoacoustic
★ hemoglobin
★ concentration

論文目次

中文摘要.........................................................i
英文摘要.........................................................ii
誌謝..............................................................iii
目錄..............................................................iv
圖目錄...........................................................vii
表目錄..........................................................xi

中文摘要....................i
英文摘要....................ii
誌謝.......................iii
目錄.......................iv
圖目錄....................vii
表目錄....................xi

一、緒論
1-1 研究動機.................1
1-2 文獻回顧.................2
1-2-1 比色法(Colorimetry)相關研究.................2
1-2-2 漫反射光譜分析(DRS & HSI)相關研究.................3
1-2-3 脈衝光熱輻射量測相關研究(PPTR).................6
1-2-4 光聲系統.................7
1-2-5 光聲系統種類介紹.................8
1-3 論文架構.................16

二、實驗原理
2-1 光聲效應原理.................17
2-1-1 脈衝雷射與皮膚組織間之交互作用.................17
2-1-2 光致聲波原理講解.................18
2-1-3 一般光聲方程式.................19
2-1-4 一般光聲方程式求解.................20
2-1-5 光聲效應中之非線性現象.................21
2-1-6 OR-PAM光聲顯微鏡系統解析度.................26

三、研究方法
3-1 實驗系統架構介紹.................28
3-2 雷射控制人機操作介面.................30
3-2-1 脈衝寬度.................31
3-2-2 脈衝重複率(Pulse repetition rate).................33
3-3 LabVIEW訊號擷取與紀錄.................34
3-4 樣品製作.................35
3-4-1 模具製備.................36
3-4-2 Phantom製備.................37
3-4-3 血液模擬樣品濃度調配.................38
3-5 解析度量測.................41
3-5-1 橫向解析度.................41
3-5-2 軸向解析度.................42

四、實驗結果
4-1 系統量測獲取訊號之分析.................43
4-1-1 軸向解析度量測.................44
4-1-2 橫向解析度量測.................45
4-1-3 隨Phantom厚度變化橫向解析度之量測分析.................47
4-2 系統架構對光聲訊號激發之分析.................49
4-2-1 橫向掃描.................50
4-2-2 縱向掃描.................52
4-3 光聲訊號非線性量測分析.................53
4-3-1 吸收飽和相關非線性量測.................53
4-3-2 解析度相關非線性效應量測.................55
4-4 光聲訊號隨色素濃度變化分析.................56
4-4-1 不同種類樣品下光聲訊號隨色素濃度之變化.................56
4-4-2 不同厚度下光聲訊號隨色素濃度之變化.................58
4-5 造成訊號下降幅度壓縮因素之分析.................60
4-5-1 下降雷射能量.................61
4-5-2 偏離物鏡焦點.................62
4-5-3 偏離探頭焦點.................63
4-6 隨機濃度定量準確度分析.................64

五、結論
.................70

參考文獻
.................71

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指導教授

陳思妤

審核日期

2024-7-26

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