DSpace collection: 期刊論文

α-catulin drives metastasis by activating ILK and driving an αvβ3 integrin signaling axis

title: α-catulin drives metastasis by activating ILK and driving an αvβ3 integrin signaling axis abstract: 摘要： α-Catulin is an oncoprotein that helps sustain proliferation by preventing cellular senescence. Here, we report that α-catulin also drives malignant invasion and metastasis. α-Catulin was upregulated in highly invasive non–small cell lung cancer (NSCLC) cell lines, where its ectopic expression or short-hairpin RNA–mediated attenuation enhanced or limited invasion or metastasis, respectively. α-Catulin interacted with integrin-linked kinase (ILK), a serine/threonine protein kinase implicated in cancer cell proliferation, antiapoptosis, invasion, and angiogenesis. Attenuation of ILK or α-catulin reciprocally blocked cell migration and invasion induced by the other protein. Mechanistic investigations revealed that α-catulin activated Akt-NF-κB signaling downstream of ILK, which in turn led to increased expression of fibronectin and integrin αvβ3. Pharmacologic or antibody-mediated blockade of NF-κB or αvβ3 was sufficient to inhibit α-catulin–induced cell migration and invasion. Clinically, high levels of expression of α-catulin and ILK were associated with poor overall survival in patients with NSCLC. Taken together, our study shows that α-catulin plays a critical role in cancer metastasis by activating the ILK-mediated Akt-NF-κB-αvβ3 signaling axis. Cancer Res; 73(1); 428–38. ©2012 AACR. 其他題名： Cancer Res 出版者： Philadelphia, PA: American Association for Cancer Research 出版日期： 2013-01-01 出處： Cancer research (Chicago, Ill.), 2013-01, Vol.73 (1), p.428-438 版權： 2014 INIST-CNRS 識別號： ISSN: 0008-5472 識別號： ISSN: 1538-7445 識別號： EISSN: 1538-7445 識別號： DOI: 10.1158/0008-5472.CAN-12-2095 識別號： PMID: 23047866 識別號： CODEN: CNREA8

Visibility enhancement of common bile duct for laparoscopic cholecystectomy by vivid fiber-optic indication: A porcine experiment trial

title: Visibility enhancement of common bile duct for laparoscopic cholecystectomy by vivid fiber-optic indication: A porcine experiment trial abstract: 摘要： Bile duct injury (BDI) is the most serious iatrogenic complication during laparoscopic cholecystectomy (LC) and occurs easily in inexperienced surgeons since the position of common bile duct (CBD) and its related ductal junctions are hard to precisely identify in the hepatic anatomy during surgery. BDI can be devastating, leading to chronic morbidity, high mortality, and prolonged hospitalization. In addition, it is the most frequent injury resulting in litigation and the most likely injury associated with a successful medical malpractice claim against surgeons. This study introduces a novel method for conveniently and rapidly indicating the anatomical location of CBD during LC by the direct fiber-optic illumination of 532-nm diode-pumped solid state laser through a microstructured plastic optical fiber to avoid the wrong identification of CBD and the injury from mistakenly cutting the CBD that can lead to permanent and even life threatening consequences. Six porcine were used for preliminary intra-CBD illumination experiments via laparotomy and direct duodenal incision to insert the invented CBD illumination laser catheter with nonharmful but satisfactory visual optical density. 其他題名： Biomed Opt Express 出版者： United States: Optical Society of America 出版日期： 2012-09-01 出處： Biomedical optics express, 2012-09, Vol.3 (9), p.1964-1971 資源來源： Optica 版權： 2012 Optical Society of America 2012 OSA 識別號： ISSN: 2156-7085 識別號： EISSN: 2156-7085 識別號： DOI: 10.1364/BOE.3.001964 識別號： PMID: 23024892

Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae Nannochloropsis oculata

title: Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae Nannochloropsis oculata abstract: 摘要： To promote the economic feasibility of Nannochloropsis oculata , efficacy of using polyethylene glycol (PEG) to increase microalgal growth and lipid accumulation was investigated. We first examined the effects of PEG concentrations on microalgal growth using 0–5 % (w/v) PEG-6000, and followed by exploring the effects of PEG molecular weights (400, 600, 2,000, 4,000, 6,000, and 20,000) on microalgal growth, size, as well as on yields of biomass, total lipids, and eicosapentaenoic acid. In addition, the capacity of PEG to reduce the effect of oxygen inhibition on microalgal growth was also investigated to evaluate its adaptability for use in large-scale and closed setting. Our results showed that PEG-induced osmotic stress ( Π ) in the range of 2.465–2.472 MPa can raise microalgal growth. The PEG with higher molecular weight exhibited greater efficacy of growth promotion but less lipid content under equal concentration. In this study, 0.5 % (w/v) PEG-20000 ( Π = 2.466 MPa) remarkably enhanced microalgal growth without interference of intracellular lipid productivity and cellular size, yielding >50 % (w/w) increases in biomass, total lipid, and eicosapentaenoic acid amounts after 7 days that provided the optimal condition for microalgal cultivation. These positive effects possibly resulted from the moderate enhancement of osmotic stress in the medium and stronger chaotrope-like behavior from higher molecular weight PEG. With further verification that 0.5 % (w/v) PEG-20000 enabled to reduce the effect of oxygen inhibition on microalgal growth, the PEG-20000-mediated cultivation offers a feasible means for mass culture of N. oculata in closed setting. 其他題名： Bioprocess Biosyst Eng 出版者： Berlin/Heidelberg: Springer Berlin Heidelberg 出版日期： 2014-08-01 出處： Bioprocess and biosystems engineering, 2014-08, Vol.37 (8), p.1669-1677 版權： Springer-Verlag Berlin Heidelberg 2014 識別號： ISSN: 1615-7591 識別號： ISSN: 1615-7605 識別號： EISSN: 1615-7605 識別號： DOI: 10.1007/s00449-014-1139-z 識別號： PMID: 24522612

Using fluorochemical as oxygen carrier to enhance the growth of marine microalga Nannochloropsis oculata

title: Using fluorochemical as oxygen carrier to enhance the growth of marine microalga Nannochloropsis oculata abstract: 摘要： The commercial value of marine Nannochloropsis oculata has been recognized due to its high content of eicosapentaenoic acid (>50 % w/w). To make it as a profitable bioresource, one of the most desirable goals is to develop a quality-controlled, cost-effective, and large-scale photobioreactor for N. oculata growth. Generally, closed culture system can offer many advantages over open system such as small space requirement, controllable process and low risk of contamination. However, oxygen accumulation is often a detrimental factor for enclosed microalgal culture that has seriously hampered the development of microalga-related industries. In this study, we proposed to use fluorochemical as oxygen carrier to overcome the challenge where four liquid fluorochemicals namely perfluorooctyl bromide, perfluorodecalin, methoxynonafluorobutane, and ethoxynonafluorobutane were investigated separately. Our results showed that the microalgal proliferation with different fluorinated liquids was similar and comparable to the culture without a fluorochemical. When cultured in the photobioreactor with 60 % oxygen atmosphere, the N. oculata can grow up in all the fluorochemical photobioreactors, but completely inhibited in the chamber without a fluorochemical. Moreover, the perfluorooctyl bromide system exhibited the most robust efficacy of oxygen removal in the culture media (perfluorooctyl bromide > perfluorodecalin > methoxynonafluorobutane > ethoxynonafluorobutane), and yielded a >3-fold increase of biomass production after 5 days. In summary, the developed fluorochemical photobioreactors offer a feasible means for N. oculata growth in closed and large-scale setting without effect of oxygen inhibition. 其他題名： Bioprocess Biosyst Eng 出版者： Berlin/Heidelberg: Springer Berlin Heidelberg 出版日期： 2013-08-01 出處： Bioprocess and biosystems engineering, 2013-08, Vol.36 (8), p.1071-1078 版權： Springer-Verlag Berlin Heidelberg 2012 版權： Springer-Verlag Berlin Heidelberg 2013 識別號： ISSN: 1615-7591 識別號： ISSN: 1615-7605 識別號： EISSN: 1615-7605 識別號： DOI: 10.1007/s00449-012-0860-8 識別號： PMID: 23178985