Bioadhesive control of plasma proteins and blood cells from umbilical cord blood onto the interface grafted with zwitterionic polymer brushes

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Title:	Bioadhesive control of plasma proteins and blood cells from umbilical cord blood onto the interface grafted with zwitterionic polymer brushes
Authors:	樋口亞紺;Chang, Yu;Chang, Yung;Higuchi, Akon;Shih, Yu-Ju;Li, Pei-Tsz;Chen, Wen-Yih;Tsai, Eing-Mei;Hsiue, Ging-Ho
Contributors:	工學院化學工程與材料工程學系
Keywords:	Adhesiveness;adsorption;Biocompatible Materials - chemistry;Blood Cells - cytology;Blood Cells - metabolism;blood platelets;Blood Proteins - chemistry;chemical composition;Chemistry;Enzyme-Linked Immunosorbent Assay;Exact sciences and technology;Fetal Blood - cytology;fibrinogen;General and physical chemistry;Humans;hydrophilicity;hydrophobicity;Methacrylates - chemistry;monoclonal antibodies;ozone;Polymers - chemistry;polystyrenes;stem cells;Surface physical chemistry;Surface Properties;temperature;umbilical cord;zwitterions
Date:	2012-03-06
Issue Date:	2026-04-21 13:52:34 (UTC+8)
Publisher:	American Chemical Society;Washington, DC: American Chemical Society
Abstract:	摘要： In this work, bioadhesive behavior of plasma proteins and blood cells from umbilical cord blood (UCB) onto zwitterionic poly(sulfobetaine methacrylate) (polySBMA) polymer brushes was studied. The surface coverage of polySBMA brushes on a hydrophobic polystyrene (PS) well plate with surface grafting weights ranging from 0.02 mg/cm2 to 0.69 mg/cm2 can be effectively controlled using the ozone pretreatment and thermal-induced radical graft-polymerization. The chemical composition, grafting structure, surface hydrophilicity, and hydration capability of prepared polySBMA brushes were determined to illustrate the correlations between grafting properties and blood compatibility of zwitterionic-grafted surfaces in contact with human UCB. The protein adsorption of fibrinogen in single-protein solutions and at complex medium of 100% UCB plasma onto different polySBMA brushes with different grafting coverage was measured by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. The grafting density of the zwitterionic brushes greatly affects the PS surface, thus controlling the adsorption of fibrinogen, the adhesion of platelets, and the preservation of hematopoietic stem and progenitor cells (HSPCs) in UCB. The results showed that PS surfaces grafted with polySBMA brushes possess controllable hydration properties through the binding of water molecules, regulating the bioadhesive and bioinert characteristics of plasma proteins and blood platelets in UCB. Interestingly, it was found that the polySBMA brushes with an optimized grafting weight of approximately 0.1 mg/cm2 at physiologic temperatures show significant hydrated chain flexibility and balanced hydrophilicity to provide the best preservation capacity for HSPCs stored in 100% UCB solution for 2 weeks. This work suggests that, through controlling grafting structures, the hemocompatible nature of grafted zwitterionic polymer brushes makes them well suited to the molecular design of regulated bioadhesive interfaces for use in the preservation of HSPCs from human UCB. 其他題名： Langmuir 出版者： Washington, DC: American Chemical Society 出版日期： 2012-03-06 出處： Langmuir, 2012-03, Vol.28 (9), p.4309-4317 資源來源： American Chemical Society Journals 版權： Copyright © 2012 American Chemical Society 版權： 2015 INIST-CNRS 識別號： ISSN: 0743-7463 識別號： ISSN: 1520-5827 識別號： EISSN: 1520-5827 識別號： DOI: 10.1021/la203504h 識別號： PMID: 22268580 識別號： CODEN: LANGD5
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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