Zwitterionic polymer-based platform with two-layer architecture for ultra low fouling and high protein loading

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題名:	Zwitterionic polymer-based platform with two-layer architecture for ultra low fouling and high protein loading
作者:	黃俊仁;Huang, Chun-Jen;Li, Yuting;Jiang, Shaoyi
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	acrylamides;Adsorption;Analytical chemistry;antigen detection;antigens;Betaine - chemistry;binding capacity;Binding sites;Biofouling - prevention & control;Biological and medical sciences;Biosensors;Biotechnology;blood plasma;Chemical compounds;Chemistry;coatings;Exact sciences and technology;Fouling;Fundamental and applied biological sciences. Psychology;General, instrumentation;Humans;Immobilized Proteins - chemistry;immunoglobulin G;Methods. Procedures. Technologies;Polymerization;Polymers;Polymers - chemistry;Proteins;surface plasmon resonance;Thyroid;thyrotropin;Thyrotropin - chemistry;ultrastructure;Various methods and equipments;Water - chemistry;zwitterions
日期:	2012-04-03
上傳時間:	2026-04-21 13:54:03 (UTC+8)
出版者:	American Chemical Society;Washington, DC: American Chemical Society
摘要:	摘要： High resistance to nonspecific adsorption typically accompanies loss of binding capacity and vice versa for many surface coatings and applications. In this study, a zwitterionic polycarboxybetaine acrylamide (pCB)-based binding platform with a “two-layer” structure for ultra low fouling and high protein loading properties was developed. The first pCB layer with a high packing density prepared under a water-free condition serves as a protective layer to resist nonspecific adsorption from complex media. The second pCB layer with a low packing density is used to achieve high protein binding capacity. Amounts of tetraethylthiuram disulfide (TED) and water in the reaction were varied to regulate the packing density and chain length of polymers, respectively, for the second pCB layer. The in situ modification of pCB films with antihuman thyroid stimulating hormone (TSH) IgG molecules and the detection of TSH antigens were employed to demonstrate high protein immobilization and high antigen detection capabilities of this “two-layer” structure. Undiluted blood plasma was used to test the nonfouling properties of this platform. Nonspecific and specific interactions were monitored by a surface plasmon resonance sensor. This work demonstrates great promise of this “two-layer” binding platform for the improved performance of biosensors. 其他題名： Anal. Chem 出版者： Washington, DC: American Chemical Society 出版日期： 2012-04-03 出處： Analytical chemistry (Washington), 2012-04, Vol.84 (7), p.3440-3445 資源來源： American Chemical Society (ACS) - Revues 版權： Copyright © 2012 American Chemical Society 版權： 2015 INIST-CNRS 版權： Copyright American Chemical Society Apr 3, 2012 識別號： ISSN: 0003-2700 識別號： ISSN: 1520-6882 識別號： EISSN: 1520-6882 識別號： DOI: 10.1021/ac3003769 識別號： PMID: 22409836 識別號： CODEN: ANCHAM
顯示於類別:	[化學工程與材料工程學系 ] 期刊論文

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