Effect of the surface density of nanosegments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood

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Title:	Effect of the surface density of nanosegments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood
Authors:	樋口亞紺;Chen, Li-Ying;Chang, Yung;Shiao, Jui-Shiang;Ling, Qing-Dong;Chang, Yu;Chen, Yung Hung;Chen, Da-Chung;Hsu, Shih-Tien;Lee, Henry Hsin-chung;Higuchi, Akon
Contributors:	工學院化學工程與材料工程學系
Keywords:	Batch Cell Culture Techniques;Batch Cell Culture Techniques - methods;biocompatible materials;blood;Bulging;Cell culture;Cells, Cultured;Cells, Immobilized;chemistry;contact angle;Culture;cytology;Density;Extracellular matrix;Fetal Blood;Fetal Blood - cytology;fibronectins;Grafting;Hematopoietic Stem Cells;Hematopoietic Stem Cells - cytology;Hematopoietic Stem Cells - physiology;Humans;macrophages;Materials Testing;methods;Nanomaterials;Nanostructure;Nanostructures;Nanostructures - chemistry;patients;physiology;polyamines;RGD peptide;stem cells;Surface modification;Surface Properties;surface roughness;Tissue Engineering;Tissue Engineering - methods;Transplantation;umbilical cord;Umbilical cords
Date:	2012-05-01
Issue Date:	2026-04-21 13:54:27 (UTC+8)
Publisher:	Elsevier BV;England: Elsevier Ltd
Abstract:	摘要： Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells for hematopoietic stem cell (HSC) transplantation. However, the low number of HSCs obtainable from a single donor of UCB limits direct transplantation of UCB to the treatment of pediatric patients. In this study, we investigated the ex vivo expansion of HSCs cultured on biomaterials grafted with several nanosegments, i.e. polyamine, fibronectin, RGDS, and CS1 (EILDVPST), at several surface densities. No direct correlation was found between fold expansion of HSCs and physical parameters of the culture dishes, i.e. surface roughness and water contact angle of the culture dishes. However, a small amount of grafted amino groups, less than 0.8residualμmolcm−2, on the dishes was effective for the ex vivo expansion of HSCs. A high amount of grafted amino groups hindered the ex vivo expansion of HSCs on the dishes. HSCs cultured on dishes with a high concentration of CS1 (2.40residualμmolcm−2) showed greater expansion of HSCs and more pluripotent colony-forming units (i.e. colony-forming unit–granulocyte, erythroid, macrophage, and megakaryocyte (CFU-GEMM)) than those on fibronectin-grafted and polyamine-grafted dishes. These data suggest that the specific interaction between HSCs and CS1 helps to maintain the pluripotency of HSCs during the ex vivo expansion of HSCs. 其他題名： Acta Biomater 出版者： England: Elsevier Ltd 出版日期： 2012-05-01 出處： Acta Biomaterialia, 2012-05, Vol.8 (5), p.1749-1758 版權： 2012 Acta Materialia Inc. 版權： Copyright © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. 識別號： ISSN: 1742-7061 識別號： ISSN: 1878-7568 識別號： EISSN: 1878-7568 識別號： DOI: 10.1016/j.actbio.2012.01.002 識別號： PMID: 22281943
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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