摘要： In this study, the relationship of the structural stability of peptide diastereomers in elution solvents and their retention behaviors in reversed-phase chromatography (RPC) was examined to provide guidance on the solvent selection for a better separation of peptide diastereomers. We investigated the chromatographic retention behaviors of exenatide, a peptide drug for the treatment of type II diabetes mellitus and its three diastereomers using RPC and implicit molecular dynamics (MD) simulation analysis. Three diastereomers involved in the single serine residue mutation of d -form at the 11th, 32nd, and 39th residues were investigated in this study. Results show that the order of the solution structural stability of exenatide and its diastereomers is consistent with their retention order by 36 % acetonitrile/water elution. The sample loading solvent also affects the retention behaviors of exenatide peptide diastereomers in RPC column. Furthermore, a larger solution conformation energy difference of the critical pair of exenatide and its diastereomer ( d -Ser39) at the elution solvent of 32 % tetrahydrofuran/water were obtained by MD simulation, and baseline separation was proved experimentally. In summary, we demonstrated that the solution structural stability–chromatographic retention relationship could be a powerful tool for elution solvent selection in peptide chromatographic purification, especially valuable for the separation of critical pair of diastereomers. Figure The structural stability and reversed-phase chromatography (RPC) retention relationship was investigated for a better chromatographic separation of peptides. Our results revealed that the rigid peptide with lower solution conformation energy exhibits a smaller retention factor in RPC column. Conversely, the flexible peptide with the higher solution conformation energy exhibits a larger retention factor. Based on this finding, we have examined that the baseline separation could be achieved by tuning the elution solvent composition to increase the structural stability difference between peptides. Consequently, the structural stability and RPC retention relationship could actually provide an important guidance on peptide separation. 其他題名： Anal Bioanal Chem 出版者： Berlin/Heidelberg: Springer-Verlag 出版日期： 2012-11 出處： Analytical and bioanalytical chemistry, 2012-11, Vol.404 (8), p.2437-2444 資源來源： EBSCOhost Academic Search Premier 版權： Springer-Verlag 2012 版權： COPYRIGHT 2012 Springer 識別號： ISSN: 1618-2642 識別號： ISSN: 1618-2650 識別號： EISSN: 1618-2650 識別號： DOI: 10.1007/s00216-012-6352-5 識別號： PMID: 22945556
