Development of a competitive ELISA for the detection of a furaltadone marker residue, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), in cultured fish samples

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/100467

Title:	Development of a competitive ELISA for the detection of a furaltadone marker residue, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), in cultured fish samples
Authors:	李文仁;TAI, Yung-Te;LEI, Yi-Chih;LI, Wen-Ren;HSIEH, Kuan-Huei;CHANG, Tong-Hsuan;LIN, Chiu-Yueh;CHENG, Chu-Chen;SHEU, Shi-Yuan;KUO, Tzong-Fu
Contributors:	理學院化學學系
Keywords:	3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ);Animals;Benzaldehydes;Biomarkers - analysis;Biomarkers - metabolism;Drug Residues - analysis;Drug Residues - chemistry;Drug Residues - metabolism;ELISA;Enzyme-Linked Immunosorbent Assay - methods;Enzyme-Linked Immunosorbent Assay - veterinary;Fishes - metabolism;furaltadone;High-Throughput Screening Assays - methods;High-Throughput Screening Assays - veterinary;Inhibitory Concentration 50;Molecular Structure;Morpholines - analysis;Morpholines - chemistry;Morpholines - metabolism;Nitrofurans;Oxazolidinones - analysis;Oxazolidinones - chemistry;Oxazolidinones - metabolism;residue;Sensitivity and Specificity
Date:	2012-12-17
Issue Date:	2026-04-21 14:03:34 (UTC+8)
Publisher:	Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE
Abstract:	摘要： This report describes an enzyme-linked immunosorbent assay (ELISA) for tissue-bound metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and the application to residue analysis in cultured fish samples. The residue is monitored as a marker for the drug furaltadone. The assay enables the detection of protein bound AMOZ in the form of a 2-nitrophenyl derivative (2-NP-AMOZ) in sample supernatant or extract after acid hydrolysis and derivatization with o-nitrobenzaldehyde. Polyclonal rabbit antibodies were produced with a new immunogen hapten, 2-NP-HXA-AMOZ. The new ELISA had adequate analytical sensitivity (IC50 value 0.325 μg kg-1; limit of detection 0.1 μg kg-1) to determine a trace of AMOZ residue and had a high selectivity. Recoveries of AMOZ fortified at the levels of 0.1, 0.5 and 1.0 μg kg-1 ranged from 89.8 to 112.5% with coefficients of variation of 12.4-16.2% over the range of AMOZ concentrations studied. The results obtained with the ELISA correlated well with those obtained by commercial test kits for 150 tested samples (r=0.984). The results suggest that the developed ELISA is a highly specific, accurate, and sensitive method suitable for high throughput screening for AMOZ residues. 其他題名： J. Vet. Med. Sci. 出版者： Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE 出版日期： 2012 出處： Journal of Veterinary Medical Science, 2012, Vol.74(11), pp.1439-1446 資源來源： J-STAGE Free 版權： 2012 by the Japanese Society of Veterinary Science 版權： Copyright Japan Science and Technology Agency 2012 識別號： ISSN: 1347-7439 識別號： ISSN: 0916-7250 識別號： EISSN: 1347-7439 識別號： DOI: 10.1292/jvms.12-0165 識別號： PMID: 22785246
Appears in Collections:	[Department of Chemistry] journal & Dissertation

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	16	View/Open

社群 sharing

Loading...