Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging

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Title:	Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging
Authors:	黃俊仁;Chen, Wen-Yih;Chen, Hon-Chen;Yang, Yuh-Shyong;Huang, Chun-Jen;Chan, Hardy Wai-Hong;Hu, Wen-Pin
Contributors:	工學院化學工程與材料工程學系
Keywords:	Biological and medical sciences;Biosensing Techniques - instrumentation;Biosensors;Biotechnology;Conductometry - instrumentation;DNA;DNA - analysis;DNA - genetics;DNA analog;DNA microarrays;DNA probes;DNA Probes - genetics;Equipment Design;Equipment Failure Analysis;Ethylated DNA;Field-effect transistor;Fundamental and applied biological sciences. Psychology;gene expression;image analysis;Methods. Procedures. Technologies;Molecular Probe Techniques - instrumentation;nanowires;neutralization;Reproducibility of Results;Sensitivity and Specificity;silicon;SPR imaging;Static Electricity;surface plasmon resonance;Surface Plasmon Resonance - instrumentation;Transistors, Electronic;Various methods and equipments
Date:	2013-03-15
Issue Date:	2026-04-21 14:09:22 (UTC+8)
Publisher:	Elsevier Ltd.;Kidlington: Elsevier B.V
Abstract:	摘要： Intensive efforts have been focused on the development of ultrasensitive DNA biosensors capable of quantitative gene expression analysis. Various neutralized nucleic acids have been demonstrated as alternative and attractive probe for the design of a DNA chip. However, the mechanism of the improvements has not been clearly revealed. In this investigation, we used a newly developed neutral ethylated DNA (E-DNA), a DNA analog with the “RO-P-O” backbone (wherein R could be methyl, ethyl, aryl, or alkyl group) obtained from synthetic procedures, and a silicon nanowire (SiNW) field-effect transistor (FET) to evaluate the difference in DNA detection performance while using E-DNA and DNA as probes. It is demonstrated that using the E-DNA probe in the FET measurement could have a significantly enhanced effect upon the detection sensitivity. Surface plasmon resonance imaging (SPRi) was used to evidence the mechanism of the improved detection sensitivity. SPRi analysis showed the amounts of probe immobilization on the sensor surface and the hybridization efficiency were both enhanced with the use of E-DNA. Consequently, neutral ethylated DNA probe hold a great promise for DNA sensing, especially in the electrical-based sensor. ► The E-DNA probe provides a larger response and a higher S/N ratio in the FET measurement. ► The electrically neutral E-DNA probes obviously improve the FET sensor sensitivity. ► Probe immobilization amount and hybridization efficiency can be enhanced by using the E-DNA probe. ► The E-DNA probe is a very suitable alternative as a DNA probe in the gene detection. 其他題名： Biosens Bioelectron 出版者： Kidlington: Elsevier B.V 出版日期： 2013-03-15 出處： Biosensors & bioelectronics, 2013-03, Vol.41, p.795-801 版權： 2012 Elsevier B.V. 版權： 2014 INIST-CNRS 版權： Copyright © 2012 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： ISSN: 1873-4235 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2012.10.010 識別號： PMID: 23116544
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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