Kosmotrope-like hydration behavior of polyethylene glycol from microcalorimetry and binding isotherm measurements

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Title:	Kosmotrope-like hydration behavior of polyethylene glycol from microcalorimetry and binding isotherm measurements
Authors:	陳文逸;Chen, Wen-Yih;Hsu, Min-Yen;Tsai, Ching-Wei;Chang, Yung;Ruaan, Rouh-Chyu;Kao, Wei-Hung;Huang, E-Wen;Chung Chuan, Hsiao-Yeh-Tzu
Contributors:	工學院化學工程與材料工程學系
Keywords:	ammonium sulfate;Applied sciences;aqueous solutions;binding capacity;biofouling;buffers;Calorimetry;chemical bonding;drugs;electrostatic interactions;enthalpy;entropy;Exact sciences and technology;lysozyme;moieties;molecular weight;Muramidase - chemistry;Muramidase - metabolism;Organic polymers;Physicochemistry of polymers;polyethylene glycol;Polyethylene Glycols - chemistry;Properties and characterization;sodium chloride;solutes;Thermodynamics;Water - chemistry
Date:	2013-04-02
Issue Date:	2026-04-21 14:09:36 (UTC+8)
Publisher:	American Chemical Society;Washington, DC: American Chemical Society
Abstract:	摘要： Polyethylene glycol (PEG) at various molecular weights (MWs) has been regarded as a wonder molecule in biomedical applications. For instance, PEG serves as a unique moiety for pegylation of “biobetter” drug development, PEG provides controlled-release and preserved activity of biologics, and PEG modified surface works as an antibiofouling surface. The primary characteristics of PEG molecules used in relevant applications have been attributed mainly to the hydration behavior in aqueous solutions. However, the effects on the solvation of solutes in solution caused by presenting PEG molecules as a cosolvent, as well as the thermodynamics aspect of the hydration behavior of PEG in solution, have not been well documented. The solvation behavior of solutes, such as protein, with PEG as a cosolvent, indicates the success of PEG applications, such as biofouling and controlled release. In this investigation, we examined the effects of a buffer solution containing PEG molecules on the solution behavior of solute and the interactions between solid surfaces with solutes. We adapted the study by selecting a lysozyme as a solute in a buffer solution with either ammonium sulfate (kosmotrope) or sodium chloride (chaotrope) and anionic resin (SP-Sepharose) as solid surfaces. The experiments primarily involved binding equilibrium measurements and thermodynamics analysis. The results revealed that, in both saline buffers, adding PEG increases the binding affinity between the lysozyme and the resin, similar to kosmotropic salt in the examined salt concentrations. The thermodynamics analyses involving microcalorimetric measurements show that the bindings are mainly driven by enthalpy, indicating that electrostatic interaction was the primary binding force under these experimental conditions. The variations of the enthalpy and entropy of the binding thermodynamics when adding PEG to different salt types in the buffer solution showed opposite behavior, and the results support the concept of kosmotrope-like behavior of PEG. The equilibrium and thermodynamics data demonstrate that PEG has a kosmotrope-like hydration behavior, and the extent of kosmotrope-like behavior depends on the molecular weight of PEG with the outcomes of various molecular weights of PEG being added to the binding solution. The results of this study provide essential knowledge for PEG as an additive (or cosolvent) in various research applications. 其他題名： Langmuir 出版者： Washington, DC: American Chemical Society 出版日期： 2013-04-02 出處： Langmuir, 2013-04, Vol.29 (13), p.4259-4265 資源來源： American Chemical Society Journals 版權： Copyright © 2013 American Chemical Society 版權： 2014 INIST-CNRS 識別號： ISSN: 0743-7463 識別號： ISSN: 1520-5827 識別號： EISSN: 1520-5827 識別號： DOI: 10.1021/la304500w 識別號： PMID: 23330911 識別號： CODEN: LANGD5
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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