Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells

NCUIR > Engineering College > Department of Chemical and Materials Engineering > journal & Dissertation > Item 987654321/100716

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Title:	Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells
Authors:	樋口亞紺;Higuchi, Akon;Lee, Henry Hsin-chung;Ling, Qing-Dong;Yu, Wan-Chun;Kao, Ta-Chun;Huang, Yi-Wei;Hung
Contributors:	工學院化學工程與材料工程學系
Keywords:	5-fluorouracil;AC133 Antigen;Analysis;Animals;Antigens;Antigens, CD - analysis;Antineoplastic Agents - pharmacology;Antineoplastic drugs;Antitumor agents;Biomarkers;Cancer;Cancer cells;Carcinoembryonic antigen;Carcinoembryonic Antigen - biosynthesis;CD133;CEA (Oncology);Cell Line, Tumor;Cell Size - drug effects;Cell Survival - drug effects;Colon;Colon cancer;colon cancer cell;Colonic Neoplasms - drug therapy;Colonic Neoplasms - metabolism;Colonic Neoplasms - pathology;Colorectal cancer;Drug development;Drug resistance;Drug Resistance, Neoplasm;Drug therapy;drug treatment;Drugs;Glycoproteins - analysis;Health aspects;Humans;Mice;Mice, SCID;Neoplastic Stem Cells - drug effects;Original Research;Peptides - analysis;Physiological aspects;Production methods;Severe combined immunodeficiency;stem cell;Stem cell transplantation;Stem cells;Tumors;Xenografts
Date:	2013-06-17
Issue Date:	2026-04-21 14:11:41 (UTC+8)
Publisher:	New Zealand: Dove Medical Press Limited
Abstract:	摘要： We evaluated the higher levels of carcinoembryonic antigen (CEA) secreted by the LoVo human colon carcinoma cells in a medium containing anticancer drugs. Drug-resistant LoVo cells were analyzed by subcutaneously xenotransplanting them into mice. The aim of this study was to evaluate whether the drug-resistant cells isolated in this study were cancer-initiating cells, known also as cancer stem cells (CSCs). The production of CEA was investigated in LoVo cells that were cultured with 0-10 mM of anticancer drugs, and we evaluated the increase in CEA production by the LoVo cells that were stimulated by anticancer drug treatment. The expression of several CSC markers in LoVo cells treated with anticancer drugs was also evaluated. Following anticancer drug treatment, LoVo cells were injected subcutaneously into the flanks of severe combined immunodeficiency mice in order to evaluate the CSC fraction. Production of CEA by LoVo cells was stimulated by the addition of anticancer drugs. Drug-resistant LoVo cells expressed lower levels of CSC markers, and LoVo cells treated with any of the anticancer drugs tested did not generate tumors within 8 weeks from when the cells were injected subcutaneously into severe combined immunodeficiency mice. These results suggest that the drug-resistant LoVo cells have a smaller population of CSCs than the untreated LoVo cells. Production of CEA by LoVo cells can be stimulated by the addition of anticancer drugs. The drug-resistant subpopulation of LoVo colon cancer cells could stimulate the production of CEA, but these cells did not act as CSCs in in vivo tumor generation experiments. 其他題名： Drug Des Devel Ther 出版者： New Zealand: Dove Medical Press Limited 出版日期： 2013-01-01 出處： Drug design, development and therapy, 2013-01, Vol.7 (default), p.491-502 資源來源： [Open Access] DOAJ 오픈액세스 저널 디렉토리 版權： COPYRIGHT 2013 Dove Medical Press Limited 版權： 2013. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2013 Lee et al, publisher and licensee Dove Medical Press Ltd 2013 識別號： ISSN: 1177-8881 識別號： EISSN: 1177-8881 識別號： DOI: 10.2147/DDDT.S45890 識別號： PMID: 23818760
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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