The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells

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Title:	The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
Authors:	樋口亞紺;Kumar, Suresh S.;Hsiao, Jui-Hsiang;Ling, Qing-Dong;Dulinska-Molak, Ida;Chen, Guoping;Chang, Yung;Chang, Yu;Chen, Yung Hung;Chen, Da-Chung;Hsu, Shih-Tien;Higuchi, Akon
Contributors:	工學院化學工程與材料工程學系
Keywords:	Advanced Basic Science;biocompatible materials;Blood;Cell Proliferation;Dentistry;elasticities;Elasticity;Fibronectin;Fibronectins;Fibronectins - metabolism;Hematopoietic Stem Cells;Hematopoietic Stem Cells - cytology;Humans;Oligopeptides;Oligopeptides - metabolism;patients;polystyrenes;Stem cell;Stem Cells;Stem Cells - cytology;storage modulus;Surface grafting;tissue culture;umbilical cord
Date:	2013-10-01
Issue Date:	2026-04-21 14:15:29 (UTC+8)
Publisher:	Elsevier BV;Netherlands: Elsevier Ltd
Abstract:	摘要： Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenvironment on HSPC expansion, we investigated the ex vivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. Polyvinylalcohol-co-itaconic acid (PVA–IA)-coated dishes with different stiffnesses ranging from a 3.7 kPa to 30.4 kPa storage modulus were used. Fibronectin or an oligopeptide (CS1, EILDVPST) was grafted onto the PVA–IA substrates. High ex vivo fold expansion of HSPCs was observed in the PVA–IA dishes grafted with fibronectin or CS1, which displayed an intermediate stiffness ranging from 12.2 kPa to 30.4 kPa. The fold expansion was more than 1.4 times higher than that cultured in tissue culture polystyrene dishes (TCPS, 12 GPa). Furthermore, HSPCs cultured in fibronectin or CS1-grafted PVA–IA-coated dishes with a stiffness of 12.2–30.4 kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the ex vivo expansion of HSPCs. 其他題名： Biomaterials 出版者： Netherlands: Elsevier Ltd 出版日期： 2013-10-01 出處： Biomaterials, 2013-10, Vol.34 (31), p.7632-7644 版權： 2013 Elsevier Ltd 版權： Elsevier Ltd 版權： Copyright © 2013 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0142-9612 識別號： ISSN: 1878-5905 識別號： EISSN: 1878-5905 識別號： DOI: 10.1016/j.biomaterials.2013.07.002 識別號： PMID: 23876761
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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