SPR bacterial pathogen biosensor: The importance of fluidic conditions and probing depth

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Title:	SPR bacterial pathogen biosensor: The importance of fluidic conditions and probing depth
Authors:	黃俊仁;Huang, Chun Jen;Knoll, Wolfgang;Sessitsch, Angela;Dostalek, Jakub
Contributors:	工學院化學工程與材料工程學系
Keywords:	Bacteria;Bacterial pathogen;Biosensing Techniques - methods;Biosensor;biosensors;Diffusion limited mass transfer;diffusivity;electromagnetic field;Electromagnetic Phenomena;epitopes;Escherichia coli K12 - chemistry;Escherichia coli K12 - metabolism;liquids;Long range surface plasmon;pathogens;Shear stress;Surface plasmon resonance;Surface Plasmon Resonance - methods
Date:	2014-01-01
Issue Date:	2026-04-21 14:21:04 (UTC+8)
Publisher:	Elsevier;Netherlands: Elsevier B.V
Abstract:	摘要： The sensitivity of surface plasmon resonance (SPR) biosensor technology for detection of bacterial analytes is investigated as a function of (a) sample flow conditions and (b) depth of probing electromagnetic field. These parameters are extremely important as such analytes exhibit large (of around micrometer) size which significantly hinders their diffusion-driven transfer from a liquid sample to the sensor and their subsequent specific capture by attached recognition elements. This is due to small diffusion coefficient and strong shear stress that decreases the stability of bonds between the bacterium specific epitope and recognition elements immobilized at the sensor surface. The importance of accurate control of sample flow conditions and probing depth in order to maximize SPR sensor response is experimentally demonstrated and supported by an analytical theory. The tuning of the probing depth of surface plasmon evanescent field to match the size of the target analyte is pursued by using long range surface plasmons. [Display omitted] •SPR biosensor sensitivity for surface plasmon modes with tunable probing depth is investigated for detection of large bacterial analytes.•The SPR sensor response is related to the surface density of captured cells.•The effect of fluidic conditions on the competing of diffusion-driven mass transfer and shear stress release of captured analyte is discussed and supported by analytical theory. 其他題名： Talanta 出版者： Netherlands: Elsevier B.V 出版日期： 2014-05-01 出處： Talanta (Oxford), 2014-05, Vol.122, p.166-171 版權： 2014 Elsevier B.V. 版權： Copyright © 2014 Elsevier B.V. All rights reserved. 識別號： ISSN: 0039-9140 識別號： ISSN: 1873-3573 識別號： EISSN: 1873-3573 識別號： DOI: 10.1016/j.talanta.2014.01.022 識別號： PMID: 24720979
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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