The effect of alginate on DNA delivery from layer-by-layer assembled films

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題名:	The effect of alginate on DNA delivery from layer-by-layer assembled films
作者:	胡威文;Hu, Wei-Wen;Tsou, Shiang-Lung
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	adjuvants;adsorption;Alginate;Alginates - chemistry;Animals;Applied sciences;Biological and medical sciences;DNA;DNA - chemistry;DNA - genetics;Drug Carriers - chemistry;electrolytes;electrostatic interactions;Exact sciences and technology;Gene delivery;gene expression;General pharmacology;Glucuronic Acid - chemistry;Hexuronic Acids - chemistry;In situ transfection;Layer-by-layer assembly;Materials Testing;Medical sciences;Mice;Natural polymers;NIH 3T3 Cells;Pharmaceutical technology. Pharmaceutical industry;Pharmacology. Drug treatments;Physicochemistry of polymers;Polyelectrolyte multilayer;Polyethyleneimine - chemistry;quartz;Starch and polysaccharides;tissue engineering;tissue scaffolds;Transfection
日期:	2014-01-01
上傳時間:	2026-04-21 14:21:37 (UTC+8)
出版者:	Elsevier Ltd.;Kidlington: Elsevier Ltd
摘要:	摘要： •Free alginate weakens the interaction within polyelectrolyte multilayers (PEMs).•Alginate can be immobilized with DNA and polycations by the layer-by-layer method.•The incorporation of alginate to PEM increases the deposition and delivery of DNA.•The existence of alginate highly improves in situ transfection on PEMs. Alginate has been previously utilized as an adjuvant in nanoparticle-mediated gene delivery. In this study, we investigated the extent to which alginate promotes in situ transfection from polyelectrolyte multilayers (PEMs). After spiking free alginate molecules, DNA release from PEMs was increased and transgene expression was enhanced, suggesting that alginate may compete with DNA to weaken electrostatic interaction within PEMs. Consequently, alginate was applied to compose multilayers with DNA and polyethyleneimine (PEI). Interestingly, the incorporated alginate increased not only deposition but also delivery of DNA from PEMs. Quartz crystal microbalance (QCM) analysis suggested that the deposited alginate enhanced PEI adsorption which in turn augmented subsequent DNA deposition. In situ transfection experiments revealed that alginate within PEMs improved not only the level but also the duration of transgene expression. This system should be a potential strategy to regulate gene delivery from scaffolds for tissue engineering application. 其他題名： Carbohydr Polym 出版者： Kidlington: Elsevier Ltd 出版日期： 2014-01-30 出處： Carbohydrate polymers, 2014-01, Vol.101, p.240-248 版權： 2013 Elsevier Ltd 版權： 2015 INIST-CNRS 版權： Copyright © 2013 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0144-8617 識別號： ISSN: 1879-1344 識別號： EISSN: 1879-1344 識別號： DOI: 10.1016/j.carbpol.2013.09.025 識別號： PMID: 24299770 識別號： CODEN: CAPOD8
顯示於類別:	[化學工程與材料工程學系 ] 期刊論文

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