The regulation of DNA adsorption and release through chitosan multilayers

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Title:	The regulation of DNA adsorption and release through chitosan multilayers
Authors:	陳文逸;Hu, Wei-Wen;Chen, Yung-Jen;Ruaan, Ruoh-Chyu;Chen, Wen-Yih;Cheng, Yu-Che;Chien, Chih-Cheng
Contributors:	工學院化學工程與材料工程學系
Keywords:	Adsorption;Applied sciences;Biological and medical sciences;Chitosan;Chitosan - chemistry;DNA;DNA - chemistry;electrolytes;Escherichia coli - genetics;Escherichia coli - metabolism;Exact sciences and technology;Gene delivery;Gene Expression;gene transfer;Gene Transfer Techniques;General pharmacology;Genes, Reporter;Green Fluorescent Proteins - genetics;Green Fluorescent Proteins - metabolism;HEK293 Cells;Humans;Hydrogen-Ion Concentration;Interdiffusion;Layer-by-layer assembly;Medical sciences;Microscopy, Atomic Force;Molecular Conformation;Molecular Weight;Natural polymers;Pharmaceutical technology. Pharmaceutical industry;Pharmacology. Drug treatments;Physicochemistry of polymers;Plasmids - chemistry;Polyamines - chemistry;Polyelectrolyte multilayers;polymers;Starch and polysaccharides;Surface Properties
Date:	2014-01-01
Issue Date:	2026-04-21 14:21:58 (UTC+8)
Publisher:	Elsevier Ltd.;Kidlington: Elsevier Ltd
Abstract:	摘要： •Chitosan can immobilize DNA by layer-by-layer assembly to transfect surface cells.•DNA adsorption linearly correlates to the bilayer number and DNA concentrations.•Chitosan deposition in polyelectrolyte multilayers deceases in low pH buffer.•More DNA releases from multilayers fabricated by more acidic chitosan solutions. To sustain transgene expression, chitosan was studied to immobilize DNA using layer-by-layer assembly to form polyelectrolyte multilayers (PEMs). Higher DNA concentrations and longer deposition periods demonstrated more DNA adsorptions to PEMs. By adjusting pH and the molecular weight of chitosan, PEM structures were manipulated. Chitosan molecules adsorption to PEMs increased when they were at pH 6 because of their low protonation. Furthermore, the configuration of chitosan favored a coiled-form when the pH was high, as the intramolecular repulsion decreased. Therefore, interdiffusion of polyelectrolytes in PEMs was promoted to increase DNA adsorption, especially for chitosan with high molecular weight. For the release experiments, because PEMs fabricated by lower pH chitosan owned less chitosan molecules, DNA release was enhanced. However, this phenomenon did not happen to chitosan with high molecular weight, which should be due to the entanglement between polymer chains. This comprehensive approach should be beneficial to substrate-mediated gene delivery applications. 其他題名： Carbohydr Polym 出版者： Kidlington: Elsevier Ltd 出版日期： 2014 出處： Carbohydrate polymers, 2014, Vol.99, p.394-402 版權： 2013 Elsevier Ltd 版權： 2015 INIST-CNRS 版權： Copyright © 2013 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0144-8617 識別號： ISSN: 1879-1344 識別號： EISSN: 1879-1344 識別號： DOI: 10.1016/j.carbpol.2013.08.088 識別號： PMID: 24274523 識別號： CODEN: CAPOD8
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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